Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria
This study aimed to assess the diversity and antimicrobial activity of cultivable bacteria associated with Vietnamese sponges. In total, 460 bacterial isolates were obtained from 18 marine sponges. Of these, 58.3% belonged to <i>Proteobacteria</i>, 16.5% to <i>Actinobacteria</i&...
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MDPI AG
2021-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/7/353 |
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| author | Ton That Huu Dat Nguyen Thi Kim Cuc Pham Viet Cuong Hauke Smidt Detmer Sipkema |
| author_facet | Ton That Huu Dat Nguyen Thi Kim Cuc Pham Viet Cuong Hauke Smidt Detmer Sipkema |
| author_sort | Ton That Huu Dat |
| collection | DOAJ |
| description | This study aimed to assess the diversity and antimicrobial activity of cultivable bacteria associated with Vietnamese sponges. In total, 460 bacterial isolates were obtained from 18 marine sponges. Of these, 58.3% belonged to <i>Proteobacteria</i>, 16.5% to <i>Actinobacteria</i>, 18.0% to <i>Firmicutes</i>, and 7.2% to <i>Bacteroidetes</i>. At the genus level, isolated strains belonged to 55 genera, of which several genera, such as <i>Bacillus</i>, <i>Pseudovibrio</i>, <i>Ruegeria</i>, <i>Vibrio</i>, and <i>Streptomyces</i>, were the most predominant. Culture media influenced the cultivable bacterial composition, whereas, from different sponge species, similar cultivable bacteria were recovered. Interestingly, there was little overlap of bacterial composition associated with sponges when the taxa isolated were compared to cultivation-independent data. Subsequent antimicrobial assays showed that 90 isolated strains exhibited antimicrobial activity against at least one of seven indicator microorganisms. From the culture broth of the isolated strain with the strongest activity (<i>Bacillus</i> sp. M1_CRV_171), four secondary metabolites were isolated and identified, including cyclo(L-Pro-L-Tyr) (<b>1</b>), macrolactin A (<b>2</b>), macrolactin H (<b>3</b>), and 15,17-epoxy-16-hydroxy macrolactin A (<b>4</b>). Of these, compounds <b>2</b>-<b>4</b> exhibited antimicrobial activity against a broad spectrum of reference microorganisms. |
| first_indexed | 2024-03-10T10:11:32Z |
| format | Article |
| id | doaj.art-419b8ed07bf8423cbaad5e0b73ef6a44 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T10:11:32Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-419b8ed07bf8423cbaad5e0b73ef6a442023-11-22T01:07:15ZengMDPI AGMarine Drugs1660-33972021-06-0119735310.3390/md19070353Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated BacteriaTon That Huu Dat0Nguyen Thi Kim Cuc1Pham Viet Cuong2Hauke Smidt3Detmer Sipkema4Mientrung Institute for Scientific Research, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue City, Thua Thien Hue 531600, VietnamMientrung Institute for Scientific Research, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue City, Thua Thien Hue 531600, VietnamMientrung Institute for Scientific Research, Vietnam Academy of Science and Technology, 321 Huynh Thuc Khang, Hue City, Thua Thien Hue 531600, VietnamLaboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The NetherlandsLaboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The NetherlandsThis study aimed to assess the diversity and antimicrobial activity of cultivable bacteria associated with Vietnamese sponges. In total, 460 bacterial isolates were obtained from 18 marine sponges. Of these, 58.3% belonged to <i>Proteobacteria</i>, 16.5% to <i>Actinobacteria</i>, 18.0% to <i>Firmicutes</i>, and 7.2% to <i>Bacteroidetes</i>. At the genus level, isolated strains belonged to 55 genera, of which several genera, such as <i>Bacillus</i>, <i>Pseudovibrio</i>, <i>Ruegeria</i>, <i>Vibrio</i>, and <i>Streptomyces</i>, were the most predominant. Culture media influenced the cultivable bacterial composition, whereas, from different sponge species, similar cultivable bacteria were recovered. Interestingly, there was little overlap of bacterial composition associated with sponges when the taxa isolated were compared to cultivation-independent data. Subsequent antimicrobial assays showed that 90 isolated strains exhibited antimicrobial activity against at least one of seven indicator microorganisms. From the culture broth of the isolated strain with the strongest activity (<i>Bacillus</i> sp. M1_CRV_171), four secondary metabolites were isolated and identified, including cyclo(L-Pro-L-Tyr) (<b>1</b>), macrolactin A (<b>2</b>), macrolactin H (<b>3</b>), and 15,17-epoxy-16-hydroxy macrolactin A (<b>4</b>). Of these, compounds <b>2</b>-<b>4</b> exhibited antimicrobial activity against a broad spectrum of reference microorganisms.https://www.mdpi.com/1660-3397/19/7/353antimicrobial activitycultivable bacteriasecondary metabolitessponge-associated bacteria |
| spellingShingle | Ton That Huu Dat Nguyen Thi Kim Cuc Pham Viet Cuong Hauke Smidt Detmer Sipkema Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria Marine Drugs antimicrobial activity cultivable bacteria secondary metabolites sponge-associated bacteria |
| title | Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria |
| title_full | Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria |
| title_fullStr | Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria |
| title_full_unstemmed | Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria |
| title_short | Diversity and Antimicrobial Activity of Vietnamese Sponge-Associated Bacteria |
| title_sort | diversity and antimicrobial activity of vietnamese sponge associated bacteria |
| topic | antimicrobial activity cultivable bacteria secondary metabolites sponge-associated bacteria |
| url | https://www.mdpi.com/1660-3397/19/7/353 |
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