The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue
Abstract Background Long-term preservation of adipose tissue is crucial for clinical applications. Researchers should consider both efficiency and biosafety when choosing a cryoprotective agent (CPA) for adipose tissue preservation. Glycerol has been applied as a nontoxic CPA for multiple tissues bu...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-022-02817-z |
| _version_ | 1819210041056559104 |
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| author | Pei-Qi Zhang Poh-Ching Tan Yi-Ming Gao Xiao-Jie Zhang Yun Xie Dan-Ning Zheng Shuang-Bai Zhou Qing-Feng Li |
| author_facet | Pei-Qi Zhang Poh-Ching Tan Yi-Ming Gao Xiao-Jie Zhang Yun Xie Dan-Ning Zheng Shuang-Bai Zhou Qing-Feng Li |
| author_sort | Pei-Qi Zhang |
| collection | DOAJ |
| description | Abstract Background Long-term preservation of adipose tissue is crucial for clinical applications. Researchers should consider both efficiency and biosafety when choosing a cryoprotective agent (CPA) for adipose tissue preservation. Glycerol has been applied as a nontoxic CPA for multiple tissues but not adipose tissue. We aimed to evaluate the efficacy of glycerol as a CPA for adipose tissue cryopreservation. Methods Fresh human adipose tissues were obtained from patients who underwent liposuction and divided into 1 mL samples. Each sample was randomly mixed with 1 mL of CPA: 60–100% glycerol, 0.25 mol/L trehalose or DMSO + FBS and cryopreserved in − 196 °C liquid nitrogen for one month. After thawing and elution, the tissues were immediately evaluated for activity and structural integrity in vitro. Then, 0.2 mL of each sample was transplanted subdermally to the nude mouse dorsum and harvested after one month for histological examination to assess the effect of the cryopreserved fat in transplantation. Results After cryopreservation, the samples treated with DMSO + FBS, trehalose, 60% and 70% glycerol had a more integrated structure than the samples in other groups. Tissues preserved with 70% glycerol had the highest G3PDH activity of 24.41 ± 0.70, comparable to 24.76 ± 0.48 in fresh tissue (p > 0.05). Adipose-derived stem cells (ASC) viability, proliferation and differentiation capability were also better preserved in 70% glycerol group. In vivo analysis showed that tissue preserved with 70% glycerol had a retention rate of 52.37 ± 7.53%, significantly higher than other groups. Histological observation demonstrated better structural integrity and viability in 70% glycerol group. Compared to the DMSO + FBS and trehalose groups, the glycerol groups showed lower tissue inflammation. Conclusion Glycerol (70%) is efficient in adipose tissue cryopreservation. Glycerol-based CPAs, which are nontoxic and show biosafety, are a promising solution for clinical tissue cryopreservation. |
| first_indexed | 2024-12-23T06:04:52Z |
| format | Article |
| id | doaj.art-419bb296c9004a19aed104cd89013762 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-23T06:04:52Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-419bb296c9004a19aed104cd890137622022-12-21T17:57:34ZengBMCStem Cell Research & Therapy1757-65122022-04-0113111310.1186/s13287-022-02817-zThe effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissuePei-Qi Zhang0Poh-Ching Tan1Yi-Ming Gao2Xiao-Jie Zhang3Yun Xie4Dan-Ning Zheng5Shuang-Bai Zhou6Qing-Feng Li7Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineAbstract Background Long-term preservation of adipose tissue is crucial for clinical applications. Researchers should consider both efficiency and biosafety when choosing a cryoprotective agent (CPA) for adipose tissue preservation. Glycerol has been applied as a nontoxic CPA for multiple tissues but not adipose tissue. We aimed to evaluate the efficacy of glycerol as a CPA for adipose tissue cryopreservation. Methods Fresh human adipose tissues were obtained from patients who underwent liposuction and divided into 1 mL samples. Each sample was randomly mixed with 1 mL of CPA: 60–100% glycerol, 0.25 mol/L trehalose or DMSO + FBS and cryopreserved in − 196 °C liquid nitrogen for one month. After thawing and elution, the tissues were immediately evaluated for activity and structural integrity in vitro. Then, 0.2 mL of each sample was transplanted subdermally to the nude mouse dorsum and harvested after one month for histological examination to assess the effect of the cryopreserved fat in transplantation. Results After cryopreservation, the samples treated with DMSO + FBS, trehalose, 60% and 70% glycerol had a more integrated structure than the samples in other groups. Tissues preserved with 70% glycerol had the highest G3PDH activity of 24.41 ± 0.70, comparable to 24.76 ± 0.48 in fresh tissue (p > 0.05). Adipose-derived stem cells (ASC) viability, proliferation and differentiation capability were also better preserved in 70% glycerol group. In vivo analysis showed that tissue preserved with 70% glycerol had a retention rate of 52.37 ± 7.53%, significantly higher than other groups. Histological observation demonstrated better structural integrity and viability in 70% glycerol group. Compared to the DMSO + FBS and trehalose groups, the glycerol groups showed lower tissue inflammation. Conclusion Glycerol (70%) is efficient in adipose tissue cryopreservation. Glycerol-based CPAs, which are nontoxic and show biosafety, are a promising solution for clinical tissue cryopreservation.https://doi.org/10.1186/s13287-022-02817-zAdipose tissueCryopreservationCryoprotective agentGlycerol |
| spellingShingle | Pei-Qi Zhang Poh-Ching Tan Yi-Ming Gao Xiao-Jie Zhang Yun Xie Dan-Ning Zheng Shuang-Bai Zhou Qing-Feng Li The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue Stem Cell Research & Therapy Adipose tissue Cryopreservation Cryoprotective agent Glycerol |
| title | The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue |
| title_full | The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue |
| title_fullStr | The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue |
| title_full_unstemmed | The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue |
| title_short | The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue |
| title_sort | effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue |
| topic | Adipose tissue Cryopreservation Cryoprotective agent Glycerol |
| url | https://doi.org/10.1186/s13287-022-02817-z |
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