Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes
Certain monoterpenes produced by aromatic plants are known to have lethal and sublethal effects on insects. As there is a need to replace conventional pesticides (organophosphates, carbamates, pyrethroids) with biorational pesticides, we evaluated the behavioural and toxicological responses of the G...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2013-04-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201302-0010_Behavioural_and_toxicological_responses_of_Blattella_germanica_Dictyoptera_Blattellidae_to_monoterpenes.php |
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| author | Raúl A. ALZOGARAY Valeria SFARA Ariadna N. MORETTI Eduardo N. ZERBA |
| author_facet | Raúl A. ALZOGARAY Valeria SFARA Ariadna N. MORETTI Eduardo N. ZERBA |
| author_sort | Raúl A. ALZOGARAY |
| collection | DOAJ |
| description | Certain monoterpenes produced by aromatic plants are known to have lethal and sublethal effects on insects. As there is a need to replace conventional pesticides (organophosphates, carbamates, pyrethroids) with biorational pesticides, we evaluated the behavioural and toxicological responses of the German cockroach, Blattella germanica, exposed to the following monoterpenes, namely (+)-a-pinene, (-)-a-pinene, limonene, menthone, linalool, menthyl acetate and geraniol. Locomotor activity and repellency were quantified using a video tracking system. To study the effect on locomotor activity, larvae were exposed to filter papers treated with concentrations of monoterpenes ranging between 1.4 and 1,400 µg/cm2. Only menthyl acetate applied at a concentration of 14 µg/cm2 or higher increased the locomotor activity of the larvae. Repellency was evaluated by exposing larvae to circles of filter paper divided in two equal zones: one zone was treated with a solution of a monoterpene in acetone (14 or 140 µg/cm2) and the other with only acetone. Larvae were repelled by all the monoterpenes tested. The two concentrations of geraniol and the highest concentration of menthyl acetate and linalool were as repellent as DEET (positive control). The fumigant effect was evaluated by exposing larvae to vapour from 100 µl of pure monoterpene in a sealed container. The values of the 50% Knock-down Times (KT50), expressed in minutes, were: (+)-a-pinene: 11.8, (-)-a-pinene: 14.6, limonene: 81.0, menthone: 141.0, and linalool: 238.6. (+)-a-Pinene was as good a fumigant as dichlorvos (positive control, KT50 = 9.7 min). The least effective compounds were menthyl acetate and geraniol (both affected less than 50% of larvae after 490 min of exposure). In conclusion, (+)-a-pinene (for its fumigant effect), menthyl acetate (for its hyperactivant effect) and menthone and geraniol (for their repellent properties) could potentially be used for controlling B. germanica. |
| first_indexed | 2024-12-22T12:28:07Z |
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| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-22T12:28:07Z |
| publishDate | 2013-04-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
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| series | European Journal of Entomology |
| spelling | doaj.art-419cdbd0c93a4c6fb821f97f9e2cb6d72022-12-21T18:25:44ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292013-04-01110224725210.14411/eje.2013.037eje-201302-0010Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenesRaúl A. ALZOGARAY0Valeria SFARA1Ariadna N. MORETTI2Eduardo N. ZERBA3Centro de Investigaciones de Plagas e Insecticidas (CIPEIN-CITEDEF/CONICET), J.B. de La Salle 4397, (1603) Villa Martelli, Prov. de Buenos Aires, Argentina; e-mails: ralzogaray@hotmail.com; vsfara@citedef.gob.ar; amoretti@citedef.gob.ar; ezerba@citedef.gob.arCentro de Investigaciones de Plagas e Insecticidas (CIPEIN-CITEDEF/CONICET), J.B. de La Salle 4397, (1603) Villa Martelli, Prov. de Buenos Aires, Argentina; e-mails: ralzogaray@hotmail.com; vsfara@citedef.gob.ar; amoretti@citedef.gob.ar; ezerba@citedef.gob.arCentro de Investigaciones de Plagas e Insecticidas (CIPEIN-CITEDEF/CONICET), J.B. de La Salle 4397, (1603) Villa Martelli, Prov. de Buenos Aires, Argentina; e-mails: ralzogaray@hotmail.com; vsfara@citedef.gob.ar; amoretti@citedef.gob.ar; ezerba@citedef.gob.arCentro de Investigaciones de Plagas e Insecticidas (CIPEIN-CITEDEF/CONICET), J.B. de La Salle 4397, (1603) Villa Martelli, Prov. de Buenos Aires, Argentina; e-mails: ralzogaray@hotmail.com; vsfara@citedef.gob.ar; amoretti@citedef.gob.ar; ezerba@citedef.gob.arCertain monoterpenes produced by aromatic plants are known to have lethal and sublethal effects on insects. As there is a need to replace conventional pesticides (organophosphates, carbamates, pyrethroids) with biorational pesticides, we evaluated the behavioural and toxicological responses of the German cockroach, Blattella germanica, exposed to the following monoterpenes, namely (+)-a-pinene, (-)-a-pinene, limonene, menthone, linalool, menthyl acetate and geraniol. Locomotor activity and repellency were quantified using a video tracking system. To study the effect on locomotor activity, larvae were exposed to filter papers treated with concentrations of monoterpenes ranging between 1.4 and 1,400 µg/cm2. Only menthyl acetate applied at a concentration of 14 µg/cm2 or higher increased the locomotor activity of the larvae. Repellency was evaluated by exposing larvae to circles of filter paper divided in two equal zones: one zone was treated with a solution of a monoterpene in acetone (14 or 140 µg/cm2) and the other with only acetone. Larvae were repelled by all the monoterpenes tested. The two concentrations of geraniol and the highest concentration of menthyl acetate and linalool were as repellent as DEET (positive control). The fumigant effect was evaluated by exposing larvae to vapour from 100 µl of pure monoterpene in a sealed container. The values of the 50% Knock-down Times (KT50), expressed in minutes, were: (+)-a-pinene: 11.8, (-)-a-pinene: 14.6, limonene: 81.0, menthone: 141.0, and linalool: 238.6. (+)-a-Pinene was as good a fumigant as dichlorvos (positive control, KT50 = 9.7 min). The least effective compounds were menthyl acetate and geraniol (both affected less than 50% of larvae after 490 min of exposure). In conclusion, (+)-a-pinene (for its fumigant effect), menthyl acetate (for its hyperactivant effect) and menthone and geraniol (for their repellent properties) could potentially be used for controlling B. germanica.https://www.eje.cz/artkey/eje-201302-0010_Behavioural_and_toxicological_responses_of_Blattella_germanica_Dictyoptera_Blattellidae_to_monoterpenes.phpdictyopterablattellidaeblattella germanicagerman cockroachlocomotor activitymonoterpenesrepellencyknock-down |
| spellingShingle | Raúl A. ALZOGARAY Valeria SFARA Ariadna N. MORETTI Eduardo N. ZERBA Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes European Journal of Entomology dictyoptera blattellidae blattella germanica german cockroach locomotor activity monoterpenes repellency knock-down |
| title | Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes |
| title_full | Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes |
| title_fullStr | Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes |
| title_full_unstemmed | Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes |
| title_short | Behavioural and toxicological responses of Blattella germanica (Dictyoptera: Blattellidae) to monoterpenes |
| title_sort | behavioural and toxicological responses of blattella germanica dictyoptera blattellidae to monoterpenes |
| topic | dictyoptera blattellidae blattella germanica german cockroach locomotor activity monoterpenes repellency knock-down |
| url | https://www.eje.cz/artkey/eje-201302-0010_Behavioural_and_toxicological_responses_of_Blattella_germanica_Dictyoptera_Blattellidae_to_monoterpenes.php |
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