Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion
Abstract Glucolipotocixity induces IL-1 β secretion which impairs pancreatic β-cell insulin secretion. Ellagic acid and urolithin A have strong anti-inflammatory effect on cells. Muscadine and amla are very good sources of ellagic acid. The present study examined the effect of ellagic acid, ellagic...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | Food Production, Processing and Nutrition |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s43014-020-00023-z |
| _version_ | 1831790679144005632 |
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| author | Srikanth Earpina Karen McDonough Millicent Yeboah-Awudzi Kristina J. Cook Sita Aggarwal Jack N. Losso |
| author_facet | Srikanth Earpina Karen McDonough Millicent Yeboah-Awudzi Kristina J. Cook Sita Aggarwal Jack N. Losso |
| author_sort | Srikanth Earpina |
| collection | DOAJ |
| description | Abstract Glucolipotocixity induces IL-1 β secretion which impairs pancreatic β-cell insulin secretion. Ellagic acid and urolithin A have strong anti-inflammatory effect on cells. Muscadine and amla are very good sources of ellagic acid. The present study examined the effect of ellagic acid, ellagic acid-rich muscadine or amla extract, or urolothin A on inflammation in β cells under glucolipotoxic conditions. Rat NIT-1 β cells were incubated in glucolipotoxic conditions (33.3 mM glucose, 250 μM palmitic acid or 33.3 mM glucose + 250 μM palmitic acid with or without ellagic acid, ellagic acid-rich muscadine or amla extracts standardized to its ellagic acid content, or urolithin A). Inflammatory status was evidenced by ELISA analysis of insulin and IL-1β secretion. Ellagic acid-rich muscadine or amla extracts dose-dependently stimulated insulin secretion and down-regulated IL-1β better than pure ellagic acid, or urolithin A. Urolithin A did not statistically stimulate insulin secretion and did not inhibit IL-1β. |
| first_indexed | 2024-12-22T14:38:04Z |
| format | Article |
| id | doaj.art-419e0a0910a0470b9492ec90c89df3dd |
| institution | Directory Open Access Journal |
| issn | 2661-8974 |
| language | English |
| last_indexed | 2024-12-22T14:38:04Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Food Production, Processing and Nutrition |
| spelling | doaj.art-419e0a0910a0470b9492ec90c89df3dd2022-12-21T18:22:37ZengBMCFood Production, Processing and Nutrition2661-89742020-05-012111110.1186/s43014-020-00023-zMuscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretionSrikanth Earpina0Karen McDonough1Millicent Yeboah-Awudzi2Kristina J. Cook3Sita Aggarwal4Jack N. Losso5Chronic Degenerative Disease Prevention Laboratory, School of Nutrition and Food Science, Louisiana State University AgCenter, Louisiana State University SystemSchool of Animal Science, Louisiana State University AgCenter, Louisiana State University SystemChronic Degenerative Disease Prevention Laboratory, School of Nutrition and Food Science, Louisiana State University AgCenter, Louisiana State University SystemChronic Degenerative Disease Prevention Laboratory, School of Nutrition and Food Science, Louisiana State University AgCenter, Louisiana State University SystemWilliam Hansel Cancer Prevention, Pennington Biomedical Research Center, Louisiana State University SystemChronic Degenerative Disease Prevention Laboratory, School of Nutrition and Food Science, Louisiana State University AgCenter, Louisiana State University SystemAbstract Glucolipotocixity induces IL-1 β secretion which impairs pancreatic β-cell insulin secretion. Ellagic acid and urolithin A have strong anti-inflammatory effect on cells. Muscadine and amla are very good sources of ellagic acid. The present study examined the effect of ellagic acid, ellagic acid-rich muscadine or amla extract, or urolothin A on inflammation in β cells under glucolipotoxic conditions. Rat NIT-1 β cells were incubated in glucolipotoxic conditions (33.3 mM glucose, 250 μM palmitic acid or 33.3 mM glucose + 250 μM palmitic acid with or without ellagic acid, ellagic acid-rich muscadine or amla extracts standardized to its ellagic acid content, or urolithin A). Inflammatory status was evidenced by ELISA analysis of insulin and IL-1β secretion. Ellagic acid-rich muscadine or amla extracts dose-dependently stimulated insulin secretion and down-regulated IL-1β better than pure ellagic acid, or urolithin A. Urolithin A did not statistically stimulate insulin secretion and did not inhibit IL-1β.http://link.springer.com/article/10.1186/s43014-020-00023-zMuscadineAmlaEllagic acidNIT-1 pancreatic β-cells; glucosePalmitic acidGlucolipotoxicity |
| spellingShingle | Srikanth Earpina Karen McDonough Millicent Yeboah-Awudzi Kristina J. Cook Sita Aggarwal Jack N. Losso Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion Food Production, Processing and Nutrition Muscadine Amla Ellagic acid NIT-1 pancreatic β-cells; glucose Palmitic acid Glucolipotoxicity |
| title | Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion |
| title_full | Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion |
| title_fullStr | Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion |
| title_full_unstemmed | Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion |
| title_short | Muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β-cell dysfunction via inhibition of IL-1β and improved insulin secretion |
| title_sort | muscadine or amla extracts standardized to ellagic acid content ameliorate glucolipotoxicity associated β cell dysfunction via inhibition of il 1β and improved insulin secretion |
| topic | Muscadine Amla Ellagic acid NIT-1 pancreatic β-cells; glucose Palmitic acid Glucolipotoxicity |
| url | http://link.springer.com/article/10.1186/s43014-020-00023-z |
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