Protocol for establishing a protein interactome based on close physical proximity to a target protein within live budding yeast
Summary: Here, we present a protocol for establishing a protein interactome based on close physical proximity to a target protein within live yeast cells. We describe steps for capturing both transient and stable binders by integrating a non-natural amino acid. We detail procedures for employing a s...
| Hlavní autoři: | , , |
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| Médium: | Článek |
| Jazyk: | English |
| Vydáno: |
Elsevier
2023-12-01
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| Edice: | STAR Protocols |
| Témata: | |
| On-line přístup: | http://www.sciencedirect.com/science/article/pii/S2666166723006305 |