Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast Cancer
Background: Early detection and screening of breast cancer (BC) might help improve the prognosis of BC patients. This study evaluated the use of serum microRNAs (miRs) as non-invasive biomarkers in BC patients. Methods: Using quantitative real-time polymerase chain reaction, we evaluated the serum e...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/4/789 |
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| author | Amal Ahmed Mohamed Ahmed E. Allam Ahmed M. Aref Maha Osama Mahmoud Noha A. Eldesoky Naglaa Fawazy Yasser Sakr Mohamed Emam Sobeih Sarah Albogami Eman Fayad Fayez Althobaiti Ibrahim Jafri Ghadi Alsharif Marwa El-Sayed Asmaa Sayed Abdelgeliel Rania S. Abdel Aziz |
| author_facet | Amal Ahmed Mohamed Ahmed E. Allam Ahmed M. Aref Maha Osama Mahmoud Noha A. Eldesoky Naglaa Fawazy Yasser Sakr Mohamed Emam Sobeih Sarah Albogami Eman Fayad Fayez Althobaiti Ibrahim Jafri Ghadi Alsharif Marwa El-Sayed Asmaa Sayed Abdelgeliel Rania S. Abdel Aziz |
| author_sort | Amal Ahmed Mohamed |
| collection | DOAJ |
| description | Background: Early detection and screening of breast cancer (BC) might help improve the prognosis of BC patients. This study evaluated the use of serum microRNAs (miRs) as non-invasive biomarkers in BC patients. Methods: Using quantitative real-time polymerase chain reaction, we evaluated the serum expression of four candidate miRs (miR-155, miR-373, miR-10b, and miR-34a) in 99 Egyptian BC patients and 40 healthy subjects (as a control). The miRs expression was correlated with clinicopathological data. In addition, the sensitivity and specificity of the miRs were determined using receiver operating characteristic (ROC) curve analysis. Results: Serum miR-155, miR-373, and miR-10b expression were significantly upregulated (<i>p</i> < 0.001), while serum miR-34a was downregulated (<i>p</i> < 0.00) in nonmetastatic (M0) BC patients compared to the control group. In addition, serum miR-155 and miR-10b were upregulated in BC patients with large tumor sizes and extensive nodal involvement (<i>p</i> < 0.001). ROC curve analysis showed high diagnostic accuracy (area under the curve = 1.0) when the four miRs were combined. Serum miR-373 was significantly upregulated in the human epidermal growth factor 2–negative (<i>p</i> < 0.001), estrogen receptor–positive (<i>p</i> < 0.005), and progesterone receptor (PR)-positive (<i>p</i> < 0.024) in BC patients, and serum miR-155 was significantly upregulated in PR-negative (<i>p</i> < 0.001) BC patients while both serum miR-155 and miR-373 were positively correlated with the tumor grade. Conclusions: Circulating serum miR-155, miR-373, miR-10b, and miR-34a are potential biomarkers for early BC detection in Egyptian patients and their combination shows high sensitivity and specificity. |
| first_indexed | 2024-03-09T10:58:02Z |
| format | Article |
| id | doaj.art-419fa1185c714fac859ffccfbde05579 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T10:58:02Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-419fa1185c714fac859ffccfbde055792023-12-01T01:29:24ZengMDPI AGDiagnostics2075-44182022-03-0112478910.3390/diagnostics12040789Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast CancerAmal Ahmed Mohamed0Ahmed E. Allam1Ahmed M. Aref2Maha Osama Mahmoud3Noha A. Eldesoky4Naglaa Fawazy5Yasser Sakr6Mohamed Emam Sobeih7Sarah Albogami8Eman Fayad9Fayez Althobaiti10Ibrahim Jafri11Ghadi Alsharif12Marwa El-Sayed13Asmaa Sayed Abdelgeliel14Rania S. Abdel Aziz15Department of Biochemistry and Molecular Biology, National Hepatology and Tropical Medicine Research Institute, Cairo 11511, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, EgyptFaculty of Biotechnology, Modern Sciences and Arts University (MSA), Cairo 11511, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo 11511, EgyptDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy for Girls, Al-Azhar University, Cairo 11511, EgyptDepartment of Clinical Pathology, National Institute of Diabetes & Endocrinology, Cairo 11511, EgyptDepartment of Clinical Pathology, National Institute of Diabetes & Endocrinology, Cairo 11511, EgyptMedical Oncology Department, National Cancer Institute, Cairo 11511, EgyptDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaCollege of Clinical Laboratory Sciences, King Saud bin Abdulaziz University for Health Sciences, Jeddah 21423, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Medicine, South Valley University, Qena 83523, EgyptDepartment of Botany & Microbiology, Faculty of Science, South Valley University, Qena 83523, EgyptDepartment of Clinical Pathology, National Cancer Institute, Cairo University, Cairo 11976, EgyptBackground: Early detection and screening of breast cancer (BC) might help improve the prognosis of BC patients. This study evaluated the use of serum microRNAs (miRs) as non-invasive biomarkers in BC patients. Methods: Using quantitative real-time polymerase chain reaction, we evaluated the serum expression of four candidate miRs (miR-155, miR-373, miR-10b, and miR-34a) in 99 Egyptian BC patients and 40 healthy subjects (as a control). The miRs expression was correlated with clinicopathological data. In addition, the sensitivity and specificity of the miRs were determined using receiver operating characteristic (ROC) curve analysis. Results: Serum miR-155, miR-373, and miR-10b expression were significantly upregulated (<i>p</i> < 0.001), while serum miR-34a was downregulated (<i>p</i> < 0.00) in nonmetastatic (M0) BC patients compared to the control group. In addition, serum miR-155 and miR-10b were upregulated in BC patients with large tumor sizes and extensive nodal involvement (<i>p</i> < 0.001). ROC curve analysis showed high diagnostic accuracy (area under the curve = 1.0) when the four miRs were combined. Serum miR-373 was significantly upregulated in the human epidermal growth factor 2–negative (<i>p</i> < 0.001), estrogen receptor–positive (<i>p</i> < 0.005), and progesterone receptor (PR)-positive (<i>p</i> < 0.024) in BC patients, and serum miR-155 was significantly upregulated in PR-negative (<i>p</i> < 0.001) BC patients while both serum miR-155 and miR-373 were positively correlated with the tumor grade. Conclusions: Circulating serum miR-155, miR-373, miR-10b, and miR-34a are potential biomarkers for early BC detection in Egyptian patients and their combination shows high sensitivity and specificity.https://www.mdpi.com/2075-4418/12/4/789breast cancer patientsMicroRNAsserumearly detectionsensitivityspecificity |
| spellingShingle | Amal Ahmed Mohamed Ahmed E. Allam Ahmed M. Aref Maha Osama Mahmoud Noha A. Eldesoky Naglaa Fawazy Yasser Sakr Mohamed Emam Sobeih Sarah Albogami Eman Fayad Fayez Althobaiti Ibrahim Jafri Ghadi Alsharif Marwa El-Sayed Asmaa Sayed Abdelgeliel Rania S. Abdel Aziz Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast Cancer Diagnostics breast cancer patients MicroRNAs serum early detection sensitivity specificity |
| title | Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast Cancer |
| title_full | Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast Cancer |
| title_fullStr | Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast Cancer |
| title_full_unstemmed | Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast Cancer |
| title_short | Evaluation of Expressed MicroRNAs as Prospective Biomarkers for Detection of Breast Cancer |
| title_sort | evaluation of expressed micrornas as prospective biomarkers for detection of breast cancer |
| topic | breast cancer patients MicroRNAs serum early detection sensitivity specificity |
| url | https://www.mdpi.com/2075-4418/12/4/789 |
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