Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia
Strokes are the second most common cause of death worldwide and a leading cause of disability. Regular consumption of polyphenols has been shown to reduce the risk of suffering a cardiovascular event. For this reason, we have investigated the protective effect of <i>Salicornia ramosissima</...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/23/5077 |
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| author | Paula García-Rodríguez Feifei Ma Carmen del Río Marina Romero-Bernal Ana M. Najar María de la Luz Cádiz-Gurrea Francisco Javier Leyva-Jimenez Laura Ramiro Paloma Menéndez-Valladares Soledad Pérez-Sánchez Antonio Segura-Carretero Joan Montaner |
| author_facet | Paula García-Rodríguez Feifei Ma Carmen del Río Marina Romero-Bernal Ana M. Najar María de la Luz Cádiz-Gurrea Francisco Javier Leyva-Jimenez Laura Ramiro Paloma Menéndez-Valladares Soledad Pérez-Sánchez Antonio Segura-Carretero Joan Montaner |
| author_sort | Paula García-Rodríguez |
| collection | DOAJ |
| description | Strokes are the second most common cause of death worldwide and a leading cause of disability. Regular consumption of polyphenols has been shown to reduce the risk of suffering a cardiovascular event. For this reason, we have investigated the protective effect of <i>Salicornia ramosissima</i>, a seasonal halophyte that synthetizes high amounts of bioactive compounds, including polyphenols, in response to environmental stress. Aqueous, hydroalcoholic, and ethanolic extracts were prepared to investigate if dietary supplementation prior to ischemic challenge can prevent subsequent damage using two animal models. First, we screened the protective effect against hypoxia–reoxygenation in <i>Drosophila melanogaster</i> and observed that both ethanolic and hydroalcoholic extracts protected flies from the deleterious effects of hypoxia. Second, we confirmed the protective effect of <i>S. ramosissima</i> ethanolic extract against brain ischemia using the transient middle cerebral artery occlusion mice model. Four weeks of oral supplementation with the ethanolic extract before artery occlusion reduced infarct volume and lowered the plasma levels of the DNA peroxidant product 8-hydroxydeoxyguanosine. Phytochemical profiling of <i>S. ramosissima</i> ethanolic extract revealed 50 compounds. Thus, it represents a valuable source of bioactive compounds that show promising disease-modifying activities and could be further developed as an effective food supplement for the prevention or treatment of neurovascular disorders. |
| first_indexed | 2024-03-09T17:37:33Z |
| format | Article |
| id | doaj.art-41a096556bf84e14be87a791208fe091 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T17:37:33Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-41a096556bf84e14be87a791208fe0912023-11-24T11:51:46ZengMDPI AGNutrients2072-66432022-11-011423507710.3390/nu14235077Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral IschemiaPaula García-Rodríguez0Feifei Ma1Carmen del Río2Marina Romero-Bernal3Ana M. Najar4María de la Luz Cádiz-Gurrea5Francisco Javier Leyva-Jimenez6Laura Ramiro7Paloma Menéndez-Valladares8Soledad Pérez-Sánchez9Antonio Segura-Carretero10Joan Montaner11Neurovascular Research Laboratory, Vall d’Hebron Institute of Research (VHIR), Universitat Autònoma de Barcelona, Hospital Universitari Vall d’Hebron, Pg. Vall d’Hebron 119-129, 08035 Barcelona, SpainNeurovascular Research Laboratory, Vall d’Hebron Institute of Research (VHIR), Universitat Autònoma de Barcelona, Hospital Universitari Vall d’Hebron, Pg. Vall d’Hebron 119-129, 08035 Barcelona, SpainInstitute of Biomedicine of Seville (IBiS), Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla, 41013 Seville, SpainInstitute of Biomedicine of Seville (IBiS), Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla, 41013 Seville, SpainInstitute of Biomedicine of Seville (IBiS), Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla, 41013 Seville, SpainDepartment of Analytical Chemistry, University of Granada; Av. Fuentenueva s/n, 18071 Granada, SpainDepartment of Analytical Chemistry and Food Science and Technology, University of Castilla-La Mancha, Ronda de Calatrava, 7, 13071 Ciudad Real, SpainNeurovascular Research Laboratory, Vall d’Hebron Institute of Research (VHIR), Universitat Autònoma de Barcelona, Hospital Universitari Vall d’Hebron, Pg. Vall d’Hebron 119-129, 08035 Barcelona, SpainInstitute of Biomedicine of Seville (IBiS), Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla, 41013 Seville, SpainInstitute of Biomedicine of Seville (IBiS), Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla, 41013 Seville, SpainDepartment of Analytical Chemistry, University of Granada; Av. Fuentenueva s/n, 18071 Granada, SpainInstitute of Biomedicine of Seville (IBiS), Hospital Universitario Virgen del Rocío, CSIC, Universidad de Sevilla, 41013 Seville, SpainStrokes are the second most common cause of death worldwide and a leading cause of disability. Regular consumption of polyphenols has been shown to reduce the risk of suffering a cardiovascular event. For this reason, we have investigated the protective effect of <i>Salicornia ramosissima</i>, a seasonal halophyte that synthetizes high amounts of bioactive compounds, including polyphenols, in response to environmental stress. Aqueous, hydroalcoholic, and ethanolic extracts were prepared to investigate if dietary supplementation prior to ischemic challenge can prevent subsequent damage using two animal models. First, we screened the protective effect against hypoxia–reoxygenation in <i>Drosophila melanogaster</i> and observed that both ethanolic and hydroalcoholic extracts protected flies from the deleterious effects of hypoxia. Second, we confirmed the protective effect of <i>S. ramosissima</i> ethanolic extract against brain ischemia using the transient middle cerebral artery occlusion mice model. Four weeks of oral supplementation with the ethanolic extract before artery occlusion reduced infarct volume and lowered the plasma levels of the DNA peroxidant product 8-hydroxydeoxyguanosine. Phytochemical profiling of <i>S. ramosissima</i> ethanolic extract revealed 50 compounds. Thus, it represents a valuable source of bioactive compounds that show promising disease-modifying activities and could be further developed as an effective food supplement for the prevention or treatment of neurovascular disorders.https://www.mdpi.com/2072-6643/14/23/5077Salicorniastrokeneuroprotectionpolyphenolischemia |
| spellingShingle | Paula García-Rodríguez Feifei Ma Carmen del Río Marina Romero-Bernal Ana M. Najar María de la Luz Cádiz-Gurrea Francisco Javier Leyva-Jimenez Laura Ramiro Paloma Menéndez-Valladares Soledad Pérez-Sánchez Antonio Segura-Carretero Joan Montaner Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia Nutrients Salicornia stroke neuroprotection polyphenol ischemia |
| title | Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_full | Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_fullStr | Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_full_unstemmed | Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_short | Diet Supplementation with Polyphenol-Rich <i>Salicornia ramosissima</i> Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_sort | diet supplementation with polyphenol rich i salicornia ramosissima i extracts protects against tissue damage in experimental models of cerebral ischemia |
| topic | Salicornia stroke neuroprotection polyphenol ischemia |
| url | https://www.mdpi.com/2072-6643/14/23/5077 |
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