Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Made...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/4/267 |
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| author | Pablo Estevez Manoella Sibat José Manuel Leão-Martins Pedro Reis Costa Ana Gago-Martínez Philipp Hess |
| author_facet | Pablo Estevez Manoella Sibat José Manuel Leão-Martins Pedro Reis Costa Ana Gago-Martínez Philipp Hess |
| author_sort | Pablo Estevez |
| collection | DOAJ |
| description | Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (<i>Seriola fasciata</i>) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (<i>Bodianus scrofa</i>) caught in the Selvagens Islands (Portugal). |
| first_indexed | 2024-03-10T20:20:10Z |
| format | Article |
| id | doaj.art-41a3d7d81c1e4b45acb05f69a2f3cc98 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T20:20:10Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-41a3d7d81c1e4b45acb05f69a2f3cc982023-11-19T22:14:56ZengMDPI AGToxins2072-66512020-04-0112426710.3390/toxins12040267Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic CoastsPablo Estevez0Manoella Sibat1José Manuel Leão-Martins2Pedro Reis Costa3Ana Gago-Martínez4Philipp Hess5Biomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, SpainIfremer, DYNECO, Laboratoire Phycotoxines, Rue de l’Île d’Yeu, 44311 Nantes, FranceBiomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, SpainIPMA—Portuguese Institute of the Sea and Atmosphere, Av. Brasília, 1449-006 Lisbon, PortugalBiomedical Research Center (CINBIO), Department of Analytical and Food Chemistry, University of Vigo, Campus Universitario de Vigo, 36310 Vigo, SpainIfremer, DYNECO, Laboratoire Phycotoxines, Rue de l’Île d’Yeu, 44311 Nantes, FranceCiguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (<i>Seriola fasciata</i>) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (<i>Bodianus scrofa</i>) caught in the Selvagens Islands (Portugal).https://www.mdpi.com/2072-6651/12/4/267ciguatoxinsHRMSQ-TOFciguatera poisoningC-CTX1fragmentation pathways |
| spellingShingle | Pablo Estevez Manoella Sibat José Manuel Leão-Martins Pedro Reis Costa Ana Gago-Martínez Philipp Hess Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts Toxins ciguatoxins HRMS Q-TOF ciguatera poisoning C-CTX1 fragmentation pathways |
| title | Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
| title_full | Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
| title_fullStr | Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
| title_full_unstemmed | Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
| title_short | Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts |
| title_sort | liquid chromatography coupled to high resolution mass spectrometry for the confirmation of caribbean ciguatoxin 1 as the main toxin responsible for ciguatera poisoning caused by fish from european atlantic coasts |
| topic | ciguatoxins HRMS Q-TOF ciguatera poisoning C-CTX1 fragmentation pathways |
| url | https://www.mdpi.com/2072-6651/12/4/267 |
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