Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase
Salt stress triggers an Stt7-mediated LHCII-phosphorylation signaling mechanism similar to light-induced state transitions. However, phosphorylated LHCII, after detaching from PSII, does not attach to PSI but self-aggregates instead. Salt is a major stress factor in the growth of algae and plants. H...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1051711/full |
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| author | Elsinraju Devadasu Sai Divya Kanna Sai Divya Kanna Satyabala Neelam Ranay Mohan Yadav Srilatha Nama Parveen Akhtar Tamás F. Polgár Tamás F. Polgár Bettina Ughy Győző Garab Győző Garab Petar H. Lambrev Rajagopal Subramanyam |
| author_facet | Elsinraju Devadasu Sai Divya Kanna Sai Divya Kanna Satyabala Neelam Ranay Mohan Yadav Srilatha Nama Parveen Akhtar Tamás F. Polgár Tamás F. Polgár Bettina Ughy Győző Garab Győző Garab Petar H. Lambrev Rajagopal Subramanyam |
| author_sort | Elsinraju Devadasu |
| collection | DOAJ |
| description | Salt stress triggers an Stt7-mediated LHCII-phosphorylation signaling mechanism similar to light-induced state transitions. However, phosphorylated LHCII, after detaching from PSII, does not attach to PSI but self-aggregates instead. Salt is a major stress factor in the growth of algae and plants. Here, our study mainly focuses on the organization of the photosynthetic apparatus to the long-term responses of Chlamydomonas reinhardtii to elevated NaCl concentrations. We analyzed the physiological effects of salt treatment at a cellular, membrane, and protein level by microscopy, protein profile analyses, transcripts, circular dichroism spectroscopy, chlorophyll fluorescence transients, and steady-state and time-resolved fluorescence spectroscopy. We have ascertained that cells that were grown in high-salinity medium form palmelloids sphere-shaped colonies, where daughter cells with curtailed flagella are enclosed within the mother cell walls. Palmelloid formation depends on the presence of a cell wall, as it was not observed in a cell-wall-less mutant CC-503. Using the stt7 mutant cells, we show Stt7 kinase-dependent phosphorylation of light-harvesting complex II (LHCII) in both short- and long-term treatments of various NaCl concentrations—demonstrating NaCl-induced state transitions that are similar to light-induced state transitions. The grana thylakoids were less appressed (with higher repeat distances), and cells grown in 150 mM NaCl showed disordered structures that formed diffuse boundaries with the flanking stroma lamellae. PSII core proteins were more prone to damage than PSI. At high salt concentrations (100–150 mM), LHCII aggregates accumulated in the thylakoid membranes. Low-temperature and time-resolved fluorescence spectroscopy indicated that the stt7 mutant was more sensitive to salt stress, suggesting that LHCII phosphorylation has a role in the acclimation and protection of the photosynthetic apparatus. |
| first_indexed | 2024-04-09T19:28:42Z |
| format | Article |
| id | doaj.art-41a466428a2e4d00a38540dd673bce2c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T19:28:42Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-41a466428a2e4d00a38540dd673bce2c2023-04-05T05:09:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-04-011410.3389/fpls.2023.10517111051711Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinaseElsinraju Devadasu0Sai Divya Kanna1Sai Divya Kanna2Satyabala Neelam3Ranay Mohan Yadav4Srilatha Nama5Parveen Akhtar6Tamás F. Polgár7Tamás F. Polgár8Bettina Ughy9Győző Garab10Győző Garab11Petar H. Lambrev12Rajagopal Subramanyam13Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaInstitute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, HungaryDoctoral School of Biology, University of Szeged, Szeged, HungaryDepartment of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaInstitute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, HungaryInstitute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, Szeged, HungaryTheoretical Medicine Doctoral School, University of Szeged, Szeged, HungaryInstitute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, HungaryInstitute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, HungaryDepartment of Physics, Faculty of Science, University of Ostrava, Ostrava, CzechiaInstitute of Plant Biology, Biological Research Centre, Eötvös Loránd Research Network, Szeged, HungaryDepartment of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, IndiaSalt stress triggers an Stt7-mediated LHCII-phosphorylation signaling mechanism similar to light-induced state transitions. However, phosphorylated LHCII, after detaching from PSII, does not attach to PSI but self-aggregates instead. Salt is a major stress factor in the growth of algae and plants. Here, our study mainly focuses on the organization of the photosynthetic apparatus to the long-term responses of Chlamydomonas reinhardtii to elevated NaCl concentrations. We analyzed the physiological effects of salt treatment at a cellular, membrane, and protein level by microscopy, protein profile analyses, transcripts, circular dichroism spectroscopy, chlorophyll fluorescence transients, and steady-state and time-resolved fluorescence spectroscopy. We have ascertained that cells that were grown in high-salinity medium form palmelloids sphere-shaped colonies, where daughter cells with curtailed flagella are enclosed within the mother cell walls. Palmelloid formation depends on the presence of a cell wall, as it was not observed in a cell-wall-less mutant CC-503. Using the stt7 mutant cells, we show Stt7 kinase-dependent phosphorylation of light-harvesting complex II (LHCII) in both short- and long-term treatments of various NaCl concentrations—demonstrating NaCl-induced state transitions that are similar to light-induced state transitions. The grana thylakoids were less appressed (with higher repeat distances), and cells grown in 150 mM NaCl showed disordered structures that formed diffuse boundaries with the flanking stroma lamellae. PSII core proteins were more prone to damage than PSI. At high salt concentrations (100–150 mM), LHCII aggregates accumulated in the thylakoid membranes. Low-temperature and time-resolved fluorescence spectroscopy indicated that the stt7 mutant was more sensitive to salt stress, suggesting that LHCII phosphorylation has a role in the acclimation and protection of the photosynthetic apparatus.https://www.frontiersin.org/articles/10.3389/fpls.2023.1051711/fullacclimationChlamydomas reinhardtiimacroorganizationphotosystems 1 and 2salt stressstate transitions of photosynthetic apparatus |
| spellingShingle | Elsinraju Devadasu Sai Divya Kanna Sai Divya Kanna Satyabala Neelam Ranay Mohan Yadav Srilatha Nama Parveen Akhtar Tamás F. Polgár Tamás F. Polgár Bettina Ughy Győző Garab Győző Garab Petar H. Lambrev Rajagopal Subramanyam Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase Frontiers in Plant Science acclimation Chlamydomas reinhardtii macroorganization photosystems 1 and 2 salt stress state transitions of photosynthetic apparatus |
| title | Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase |
| title_full | Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase |
| title_fullStr | Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase |
| title_full_unstemmed | Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase |
| title_short | Long- and short-term acclimation of the photosynthetic apparatus to salinity in Chlamydomonas reinhardtii. The role of Stt7 protein kinase |
| title_sort | long and short term acclimation of the photosynthetic apparatus to salinity in chlamydomonas reinhardtii the role of stt7 protein kinase |
| topic | acclimation Chlamydomas reinhardtii macroorganization photosystems 1 and 2 salt stress state transitions of photosynthetic apparatus |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1051711/full |
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