Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritis
Oxidative stress is closely linked to the etiology of temporomandibular joint osteoarthritis. (TMJ-OA) and is an important therapeutic target. Cerium oxide nanoparticles (CNPs) have been broadly studied owing to their powerful antioxidant properties and potential preventive and therapeutic effects a...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1076240/full |
| _version_ | 1811172316388786176 |
|---|---|
| author | Liping Xiong Han Bao Size Li Deao Gu Yuyang Li Qianwen Yin Wen Li Leiying Miao Chao Liu |
| author_facet | Liping Xiong Han Bao Size Li Deao Gu Yuyang Li Qianwen Yin Wen Li Leiying Miao Chao Liu |
| author_sort | Liping Xiong |
| collection | DOAJ |
| description | Oxidative stress is closely linked to the etiology of temporomandibular joint osteoarthritis. (TMJ-OA) and is an important therapeutic target. Cerium oxide nanoparticles (CNPs) have been broadly studied owing to their powerful antioxidant properties and potential preventive and therapeutic effects against chronic diseases. The current study was designed to explore the protective effects of CNPs on the progression of TMJ-OA and their potential mechanisms. We detected the ability of CNPs to eliminate reactive oxygen species (ROS) in chondrocytes. Moreover, their protective effects on chondrocytes were detected in the level of gene and protein. Furthermore, TUNEL assay, histology and safranin O-fast green staining were used to detect the beneficial effects of CNPs on cartilage explants. The mechanism of CNPs, protecting condylar cartilage by reducing inflammation, was further explored by knocking down the Nuclear factor-erythroid 2-related factor (Nrf2) gene. CNPs could reduce the ROS levels in chondrocytes and cartilage explants and reverse the IL-1β-induced imbalance of cartilage matrix metabolism and apoptosis. The protective effects of CNPs on cartilage were lost after key antioxidant factors including Nrf2 and heme-oxygenase 1(HO-1) were significantly reduced. In conclusion, this study demonstrated for the first time that activating the Nrf2/HO-1 signaling pathway by CNPs might have therapeutic potential for TMJ-OA. |
| first_indexed | 2024-04-10T17:27:55Z |
| format | Article |
| id | doaj.art-41a717e4f8db44d080d5027aa9048011 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T17:27:55Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-41a717e4f8db44d080d5027aa90480112023-02-03T16:50:46ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-02-011110.3389/fbioe.2023.10762401076240Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritisLiping Xiong0Han Bao1Size Li2Deao Gu3Yuyang Li4Qianwen Yin5Wen Li6Leiying Miao7Chao Liu8Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Cariology and Endodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Cariology and Endodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Cariology and Endodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaDepartment of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, ChinaOxidative stress is closely linked to the etiology of temporomandibular joint osteoarthritis. (TMJ-OA) and is an important therapeutic target. Cerium oxide nanoparticles (CNPs) have been broadly studied owing to their powerful antioxidant properties and potential preventive and therapeutic effects against chronic diseases. The current study was designed to explore the protective effects of CNPs on the progression of TMJ-OA and their potential mechanisms. We detected the ability of CNPs to eliminate reactive oxygen species (ROS) in chondrocytes. Moreover, their protective effects on chondrocytes were detected in the level of gene and protein. Furthermore, TUNEL assay, histology and safranin O-fast green staining were used to detect the beneficial effects of CNPs on cartilage explants. The mechanism of CNPs, protecting condylar cartilage by reducing inflammation, was further explored by knocking down the Nuclear factor-erythroid 2-related factor (Nrf2) gene. CNPs could reduce the ROS levels in chondrocytes and cartilage explants and reverse the IL-1β-induced imbalance of cartilage matrix metabolism and apoptosis. The protective effects of CNPs on cartilage were lost after key antioxidant factors including Nrf2 and heme-oxygenase 1(HO-1) were significantly reduced. In conclusion, this study demonstrated for the first time that activating the Nrf2/HO-1 signaling pathway by CNPs might have therapeutic potential for TMJ-OA.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1076240/fullcerium oxide nanoparticlestemporomandibular jointosteoarthritisoxidative stressNrf2/HO-1 signal pathwaycartilage explant |
| spellingShingle | Liping Xiong Han Bao Size Li Deao Gu Yuyang Li Qianwen Yin Wen Li Leiying Miao Chao Liu Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritis Frontiers in Bioengineering and Biotechnology cerium oxide nanoparticles temporomandibular joint osteoarthritis oxidative stress Nrf2/HO-1 signal pathway cartilage explant |
| title | Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritis |
| title_full | Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritis |
| title_fullStr | Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritis |
| title_full_unstemmed | Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritis |
| title_short | Cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via Nrf2/HO-1 pathway in temporomandibular joint osteoarthritis |
| title_sort | cerium oxide nanoparticles protect against chondrocytes and cartilage explants from oxidative stress via nrf2 ho 1 pathway in temporomandibular joint osteoarthritis |
| topic | cerium oxide nanoparticles temporomandibular joint osteoarthritis oxidative stress Nrf2/HO-1 signal pathway cartilage explant |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1076240/full |
| work_keys_str_mv | AT lipingxiong ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT hanbao ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT sizeli ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT deaogu ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT yuyangli ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT qianwenyin ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT wenli ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT leiyingmiao ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis AT chaoliu ceriumoxidenanoparticlesprotectagainstchondrocytesandcartilageexplantsfromoxidativestressvianrf2ho1pathwayintemporomandibularjointosteoarthritis |