Partial purification and characterization of exoinulinase produced from Bacillus sp.
Inulinase are industrial food enzymes which have gained much attention in recent scenario. In this study, Inulinase producing eight bacterial colonies were isolated and screened from three different plant root tubers soil sample. Among 8 inulinase producing colonies, the higher yielding colony was s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-12-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X18300192 |
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| author | R. Ramapriya A. Thirumurugan T. Sathishkumar D.R. Manimaran |
| author_facet | R. Ramapriya A. Thirumurugan T. Sathishkumar D.R. Manimaran |
| author_sort | R. Ramapriya |
| collection | DOAJ |
| description | Inulinase are industrial food enzymes which have gained much attention in recent scenario. In this study, Inulinase producing eight bacterial colonies were isolated and screened from three different plant root tubers soil sample. Among 8 inulinase producing colonies, the higher yielding colony was selected with 25.10 U/mL for further studies. The best inulinase producing colony was identified by partial 16S rRNA gene sequence as Bacillus sp. The crude inulinase was purified by using ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE – sephacel and obtained 1.9 purification fold with total activity 293 U. The purified enzyme was subjected to characterization studies and it was found to be stable at 30–60 °C and optimum temperature was at 55 °C. The enzyme was stable at pH 3.0–7.0 and optimum pH was at 6.5. The Km and Vmax value for inulinase was found to be 0.117 mg/mL and 4.45 μmol min mg−1 respectively, demonstrate its greater affinity. Hence, this enzyme can be widely used for the production of fructose, and fructooligosaccharides, which are important ingredients in food and pharmaceutical industry. Keywords: Inulinase, Bacillus sp., Purification, Characterization, Food industry |
| first_indexed | 2024-04-24T08:50:49Z |
| format | Article |
| id | doaj.art-41a723eb94c74e1c8c184ddb27827306 |
| institution | Directory Open Access Journal |
| issn | 1687-157X |
| language | English |
| last_indexed | 2024-04-24T08:50:49Z |
| publishDate | 2018-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-41a723eb94c74e1c8c184ddb278273062024-04-16T11:58:34ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2018-12-01162363367Partial purification and characterization of exoinulinase produced from Bacillus sp.R. Ramapriya0A. Thirumurugan1T. Sathishkumar2D.R. Manimaran3Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamilnadu, IndiaCorresponding author.; Department of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamilnadu, IndiaDepartment of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamilnadu, IndiaDepartment of Biotechnology, Kumaraguru College of Technology, Coimbatore 641 049, Tamilnadu, IndiaInulinase are industrial food enzymes which have gained much attention in recent scenario. In this study, Inulinase producing eight bacterial colonies were isolated and screened from three different plant root tubers soil sample. Among 8 inulinase producing colonies, the higher yielding colony was selected with 25.10 U/mL for further studies. The best inulinase producing colony was identified by partial 16S rRNA gene sequence as Bacillus sp. The crude inulinase was purified by using ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE – sephacel and obtained 1.9 purification fold with total activity 293 U. The purified enzyme was subjected to characterization studies and it was found to be stable at 30–60 °C and optimum temperature was at 55 °C. The enzyme was stable at pH 3.0–7.0 and optimum pH was at 6.5. The Km and Vmax value for inulinase was found to be 0.117 mg/mL and 4.45 μmol min mg−1 respectively, demonstrate its greater affinity. Hence, this enzyme can be widely used for the production of fructose, and fructooligosaccharides, which are important ingredients in food and pharmaceutical industry. Keywords: Inulinase, Bacillus sp., Purification, Characterization, Food industryhttp://www.sciencedirect.com/science/article/pii/S1687157X18300192 |
| spellingShingle | R. Ramapriya A. Thirumurugan T. Sathishkumar D.R. Manimaran Partial purification and characterization of exoinulinase produced from Bacillus sp. Journal of Genetic Engineering and Biotechnology |
| title | Partial purification and characterization of exoinulinase produced from Bacillus sp. |
| title_full | Partial purification and characterization of exoinulinase produced from Bacillus sp. |
| title_fullStr | Partial purification and characterization of exoinulinase produced from Bacillus sp. |
| title_full_unstemmed | Partial purification and characterization of exoinulinase produced from Bacillus sp. |
| title_short | Partial purification and characterization of exoinulinase produced from Bacillus sp. |
| title_sort | partial purification and characterization of exoinulinase produced from bacillus sp |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X18300192 |
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