Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter.
Rapid progress has been made in terms of metal nanoparticles studied in numerous fields. Metal nanoparticles have also been used in medical research, and antibacterial properties and anticancer effects have been reported. However, the underlying mechanism responsible for these effects has not been f...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0222634 |
| _version_ | 1818590332551430144 |
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| author | Hiroo Itohiya Yuji Matsushima Satoshi Shirakawa Sohtaro Kajiyama Akihiro Yashima Takatoshi Nagano Kazuhiro Gomi |
| author_facet | Hiroo Itohiya Yuji Matsushima Satoshi Shirakawa Sohtaro Kajiyama Akihiro Yashima Takatoshi Nagano Kazuhiro Gomi |
| author_sort | Hiroo Itohiya |
| collection | DOAJ |
| description | Rapid progress has been made in terms of metal nanoparticles studied in numerous fields. Metal nanoparticles have also been used in medical research, and antibacterial properties and anticancer effects have been reported. However, the underlying mechanism responsible for these effects has not been fully elucidated. Therefore, the present study focused on platinum nanoparticles (PtNPs) and examined their antibacterial properties and functional potential for decomposing organic matter, considering potential applications in the dental field. PtNPs were allowed to react with dental-related bacteria (Streptococcus mutans; Enterococcus faecalis, caries; Porphyromonas gingivalis, and endodontic and periodontal lesions). Antibacterial properties were evaluated by measuring colony formation. In addition, PtNPs were allowed to react with albumin and lipopolysaccharides (LPSs), and the functional potential to decompose organic matter was evaluated. All evaluations were performed in vitro. Colony formation in all bacterial species was completely suppressed by PtNPs at concentrations of >5 ppm. The addition of PtNPs at concentrations of >10 ppm significantly increased fragmentation and decomposition. The addition of PtNPs at concentrations of >125 pico/mL to 1 EU/mL LPS resulted in significant amounts of decomposition and elimination. The results revealed that PtNPs had antibacterial effects against dental-related bacteria and proteolytic potential to decompose proteins and LPS, an inflammatory factor associated with periodontal disease. Therefore, the use and application of PtNPs in periodontal and endodontic treatment is considered promising. |
| first_indexed | 2024-12-16T09:54:52Z |
| format | Article |
| id | doaj.art-41a8bd9991f04e1590f517397c58ba09 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:54:52Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-41a8bd9991f04e1590f517397c58ba092022-12-21T22:35:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e022263410.1371/journal.pone.0222634Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter.Hiroo ItohiyaYuji MatsushimaSatoshi ShirakawaSohtaro KajiyamaAkihiro YashimaTakatoshi NaganoKazuhiro GomiRapid progress has been made in terms of metal nanoparticles studied in numerous fields. Metal nanoparticles have also been used in medical research, and antibacterial properties and anticancer effects have been reported. However, the underlying mechanism responsible for these effects has not been fully elucidated. Therefore, the present study focused on platinum nanoparticles (PtNPs) and examined their antibacterial properties and functional potential for decomposing organic matter, considering potential applications in the dental field. PtNPs were allowed to react with dental-related bacteria (Streptococcus mutans; Enterococcus faecalis, caries; Porphyromonas gingivalis, and endodontic and periodontal lesions). Antibacterial properties were evaluated by measuring colony formation. In addition, PtNPs were allowed to react with albumin and lipopolysaccharides (LPSs), and the functional potential to decompose organic matter was evaluated. All evaluations were performed in vitro. Colony formation in all bacterial species was completely suppressed by PtNPs at concentrations of >5 ppm. The addition of PtNPs at concentrations of >10 ppm significantly increased fragmentation and decomposition. The addition of PtNPs at concentrations of >125 pico/mL to 1 EU/mL LPS resulted in significant amounts of decomposition and elimination. The results revealed that PtNPs had antibacterial effects against dental-related bacteria and proteolytic potential to decompose proteins and LPS, an inflammatory factor associated with periodontal disease. Therefore, the use and application of PtNPs in periodontal and endodontic treatment is considered promising.https://doi.org/10.1371/journal.pone.0222634 |
| spellingShingle | Hiroo Itohiya Yuji Matsushima Satoshi Shirakawa Sohtaro Kajiyama Akihiro Yashima Takatoshi Nagano Kazuhiro Gomi Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter. PLoS ONE |
| title | Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter. |
| title_full | Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter. |
| title_fullStr | Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter. |
| title_full_unstemmed | Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter. |
| title_short | Organic resolution function and effects of platinum nanoparticles on bacteria and organic matter. |
| title_sort | organic resolution function and effects of platinum nanoparticles on bacteria and organic matter |
| url | https://doi.org/10.1371/journal.pone.0222634 |
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