Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation
This research was aimed to evaluate the effect of inoculant and crude enzyme of Trichoderma reesei on fermentation quality and rumen digestibility of napier grass silage. This research consisted of two stages. The first stage was fermentation quality using a completely randomized design with three t...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bogor Agricultural University
2016-04-01
|
| Series: | Media Peternakan |
| Subjects: | |
| Online Access: | http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/10567 |
| _version_ | 1818944891731836928 |
|---|---|
| author | D. J. Nurjana S. Suharti Suryahadi |
| author_facet | D. J. Nurjana S. Suharti Suryahadi |
| author_sort | D. J. Nurjana |
| collection | DOAJ |
| description | This research was aimed to evaluate the effect of inoculant and crude enzyme of Trichoderma reesei on fermentation quality and rumen digestibility of napier grass silage. This research consisted of two stages. The first stage was fermentation quality using a completely randomized design with three treatments and six replications. The second stage was rumen fermentability using a randomized block design with three treatments and six replications. The treatments of both stages were: T0= control of napier grass silage (Hi-fer+), T1= control + T. reesei inoculant at the level of 2.13 x 107 cfu/kg, and T2= control + crude enzyme from T. reesei at the level of 11.4 unit/kg. Napier grass silage were kept at room temperature and opened 21 d after fermentation. The result of the first stage experiment showed that T2 decreased (P<0.01) dry matter, organic matter, crude fiber, and neutral detergent fiber (NDF) and also increased (P<0.05) crude protein, total digestible nutrient (TDN), pH, acetic acid, and propionic acid of napier grass silage while T1 decreased (P<0.01) NDF and increased (P<0.05) propionic acid. The result of the second stage experiment showed that there were no significant differences in rumen pH, in vitro dry matter and organic matter digestibility, total VFA, proportion of VFA, ratio of acetic to propionic acids, and also NH3. It is concluded that the addition of crude enzyme from T. reesei could improve fermentation quality of napier grass silage with decreasing NDF and increasing TDN but did not affect rumen fermentability. |
| first_indexed | 2024-12-20T07:50:26Z |
| format | Article |
| id | doaj.art-41ae58d8892c4f3e825886087ec779a0 |
| institution | Directory Open Access Journal |
| issn | 0126-0472 2087-4634 |
| language | English |
| last_indexed | 2024-12-20T07:50:26Z |
| publishDate | 2016-04-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Media Peternakan |
| spelling | doaj.art-41ae58d8892c4f3e825886087ec779a02022-12-21T19:47:51ZengBogor Agricultural UniversityMedia Peternakan0126-04722087-46342016-04-01391465210.5398/medpet.2016.39.1.46Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen FermentationD. J. Nurjana0S. Suharti1Suryahadi2Study Program of Nutrition and Feed Science, Faculty of Animal Science, Graduate School, Bogor Agricultural University, IndonesiaDepartment of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaDepartment of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, IndonesiaThis research was aimed to evaluate the effect of inoculant and crude enzyme of Trichoderma reesei on fermentation quality and rumen digestibility of napier grass silage. This research consisted of two stages. The first stage was fermentation quality using a completely randomized design with three treatments and six replications. The second stage was rumen fermentability using a randomized block design with three treatments and six replications. The treatments of both stages were: T0= control of napier grass silage (Hi-fer+), T1= control + T. reesei inoculant at the level of 2.13 x 107 cfu/kg, and T2= control + crude enzyme from T. reesei at the level of 11.4 unit/kg. Napier grass silage were kept at room temperature and opened 21 d after fermentation. The result of the first stage experiment showed that T2 decreased (P<0.01) dry matter, organic matter, crude fiber, and neutral detergent fiber (NDF) and also increased (P<0.05) crude protein, total digestible nutrient (TDN), pH, acetic acid, and propionic acid of napier grass silage while T1 decreased (P<0.01) NDF and increased (P<0.05) propionic acid. The result of the second stage experiment showed that there were no significant differences in rumen pH, in vitro dry matter and organic matter digestibility, total VFA, proportion of VFA, ratio of acetic to propionic acids, and also NH3. It is concluded that the addition of crude enzyme from T. reesei could improve fermentation quality of napier grass silage with decreasing NDF and increasing TDN but did not affect rumen fermentability.http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/10567napier grasssilageTrichoderma reeseienzymein vitro |
| spellingShingle | D. J. Nurjana S. Suharti Suryahadi Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation Media Peternakan napier grass silage Trichoderma reesei enzyme in vitro |
| title | Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation |
| title_full | Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation |
| title_fullStr | Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation |
| title_full_unstemmed | Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation |
| title_short | Improvement of Napier Grass Silage Nutritive Value by Using Inoculant and Crude Enzyme from Trichoderma reesei and Its Effect on in Vitro Rumen Fermentation |
| title_sort | improvement of napier grass silage nutritive value by using inoculant and crude enzyme from trichoderma reesei and its effect on in vitro rumen fermentation |
| topic | napier grass silage Trichoderma reesei enzyme in vitro |
| url | http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/10567 |
| work_keys_str_mv | AT djnurjana improvementofnapiergrasssilagenutritivevaluebyusinginoculantandcrudeenzymefromtrichodermareeseianditseffectoninvitrorumenfermentation AT ssuharti improvementofnapiergrasssilagenutritivevaluebyusinginoculantandcrudeenzymefromtrichodermareeseianditseffectoninvitrorumenfermentation AT suryahadi improvementofnapiergrasssilagenutritivevaluebyusinginoculantandcrudeenzymefromtrichodermareeseianditseffectoninvitrorumenfermentation |