Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo
Background:. Three-dimensional (3D) bioprinting of cartilage is a promising new technique. To produce, for example, an auricle with good shape, the printed cartilage needs to be covered with skin that can grow on the surface of the construct. Our primary question was to analyze if an integrated 3D b...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer
2018-09-01
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| Series: | Plastic and Reconstructive Surgery, Global Open |
| Online Access: | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000001930 |
| _version_ | 1818689341014147072 |
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| author | Peter Apelgren, MD Matteo Amoroso, MD Karin Säljö, PhD Anders Lindahl, PhD Camilla Brantsing, MSc Linnéa Stridh Orrhult, PhD Paul Gatenholm, PhD Lars Kölby, PhD |
| author_facet | Peter Apelgren, MD Matteo Amoroso, MD Karin Säljö, PhD Anders Lindahl, PhD Camilla Brantsing, MSc Linnéa Stridh Orrhult, PhD Paul Gatenholm, PhD Lars Kölby, PhD |
| author_sort | Peter Apelgren, MD |
| collection | DOAJ |
| description | Background:. Three-dimensional (3D) bioprinting of cartilage is a promising new technique. To produce, for example, an auricle with good shape, the printed cartilage needs to be covered with skin that can grow on the surface of the construct. Our primary question was to analyze if an integrated 3D bioprinted cartilage structure is a tissue that can serve as a bed for a full-thickness skin graft.
Methods:. 3D bioprinted constructs (10 × 10 × 1.2 mm) were printed using nanofibrillated cellulose/alginate bioink mixed with mesenchymal stem cells and adult chondrocytes and implanted subcutaneously in 21 nude mice.
Results:. After 45 days, a full-thickness skin allograft was transplanted onto the constructs and the grafted construct again enclosed subcutaneously. Group 1 was sacrificed on day 60, whereas group 2, instead, had their skin-bearing construct uncovered on day 60 and were sacrificed on day 75 and the explants were analyzed morphologically. The skin transplants integrated well with the 3D bioprinted constructs. A tight connection between the fibrous, vascularized capsule surrounding the 3D bioprinted constructs and the skin graft were observed. The skin grafts survived the uncovering and exposure to the environment.
Conclusions:. A 3D bioprinted cartilage that has been allowed to integrate in vivo is a sufficient base for a full-thickness skin graft. This finding accentuates the clinical potential of 3D bioprinting for reconstructive purposes. |
| first_indexed | 2024-12-17T12:08:34Z |
| format | Article |
| id | doaj.art-41b100de721d46fa8931bb42a5cec96d |
| institution | Directory Open Access Journal |
| issn | 2169-7574 |
| language | English |
| last_indexed | 2024-12-17T12:08:34Z |
| publishDate | 2018-09-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Plastic and Reconstructive Surgery, Global Open |
| spelling | doaj.art-41b100de721d46fa8931bb42a5cec96d2022-12-21T21:49:31ZengWolters KluwerPlastic and Reconstructive Surgery, Global Open2169-75742018-09-0169e193010.1097/GOX.0000000000001930201809000-00011Skin Grafting on 3D Bioprinted Cartilage Constructs In VivoPeter Apelgren, MD0Matteo Amoroso, MD1Karin Säljö, PhD2Anders Lindahl, PhD3Camilla Brantsing, MSc4Linnéa Stridh Orrhult, PhD5Paul Gatenholm, PhD6Lars Kölby, PhD7From the *Department of Plastic Surgery, University of Gothenburg, The Sahlgrenska Academy, Institute of Clinical Sciences, Sahlgrenska University Hospital, Göteborg, SwedenFrom the *Department of Plastic Surgery, University of Gothenburg, The Sahlgrenska Academy, Institute of Clinical Sciences, Sahlgrenska University Hospital, Göteborg, SwedenFrom the *Department of Plastic Surgery, University of Gothenburg, The Sahlgrenska Academy, Institute of Clinical Sciences, Sahlgrenska University Hospital, Göteborg, Sweden†Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicin, Sahlgrenska University Hospital, Göteborg, Sweden†Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicin, Sahlgrenska University Hospital, Göteborg, Sweden‡Department of Chemistry and Chemical Engineering, 3D Bioprinting Centre, Chalmers University of Technology, Göteborg, Sweden.‡Department of Chemistry and Chemical Engineering, 3D Bioprinting Centre, Chalmers University of Technology, Göteborg, Sweden.From the *Department of Plastic Surgery, University of Gothenburg, The Sahlgrenska Academy, Institute of Clinical Sciences, Sahlgrenska University Hospital, Göteborg, SwedenBackground:. Three-dimensional (3D) bioprinting of cartilage is a promising new technique. To produce, for example, an auricle with good shape, the printed cartilage needs to be covered with skin that can grow on the surface of the construct. Our primary question was to analyze if an integrated 3D bioprinted cartilage structure is a tissue that can serve as a bed for a full-thickness skin graft. Methods:. 3D bioprinted constructs (10 × 10 × 1.2 mm) were printed using nanofibrillated cellulose/alginate bioink mixed with mesenchymal stem cells and adult chondrocytes and implanted subcutaneously in 21 nude mice. Results:. After 45 days, a full-thickness skin allograft was transplanted onto the constructs and the grafted construct again enclosed subcutaneously. Group 1 was sacrificed on day 60, whereas group 2, instead, had their skin-bearing construct uncovered on day 60 and were sacrificed on day 75 and the explants were analyzed morphologically. The skin transplants integrated well with the 3D bioprinted constructs. A tight connection between the fibrous, vascularized capsule surrounding the 3D bioprinted constructs and the skin graft were observed. The skin grafts survived the uncovering and exposure to the environment. Conclusions:. A 3D bioprinted cartilage that has been allowed to integrate in vivo is a sufficient base for a full-thickness skin graft. This finding accentuates the clinical potential of 3D bioprinting for reconstructive purposes.http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000001930 |
| spellingShingle | Peter Apelgren, MD Matteo Amoroso, MD Karin Säljö, PhD Anders Lindahl, PhD Camilla Brantsing, MSc Linnéa Stridh Orrhult, PhD Paul Gatenholm, PhD Lars Kölby, PhD Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo Plastic and Reconstructive Surgery, Global Open |
| title | Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo |
| title_full | Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo |
| title_fullStr | Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo |
| title_full_unstemmed | Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo |
| title_short | Skin Grafting on 3D Bioprinted Cartilage Constructs In Vivo |
| title_sort | skin grafting on 3d bioprinted cartilage constructs in vivo |
| url | http://journals.lww.com/prsgo/fulltext/10.1097/GOX.0000000000001930 |
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