Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food
ObjectiveTo establish a recombinase polymerase amplification (RPA) method for the detection of Listeria monocytogenes (L. monocytogenes) in food.MethodsBased on the hlyA gene of L. monocytogenes, a set of RPA primers was selected for constructing RPA test, and its specificity and sensitivity were th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2021-05-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/20210306?st=article_issue |
| _version_ | 1828085923977363456 |
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| author | HE Jie ZHU Chao HUANG Shanshan WU Qiaowei SUN Ping LAO Huajun |
| author_facet | HE Jie ZHU Chao HUANG Shanshan WU Qiaowei SUN Ping LAO Huajun |
| author_sort | HE Jie |
| collection | DOAJ |
| description | ObjectiveTo establish a recombinase polymerase amplification (RPA) method for the detection of Listeria monocytogenes (L. monocytogenes) in food.MethodsBased on the hlyA gene of L. monocytogenes, a set of RPA primers was selected for constructing RPA test, and its specificity and sensitivity were then tested.ResultsThe RPA assay could be finished in 30 min at 37 ℃. The primers used in RPA were specific. Experiments confirmed a detection limit of DNA template as low as 0.5 ng/μL. L. monocytogenes in the artificially-contaminated meat could be detected at the limit of 104 CFU/2.5 g.ConclusionThe RPA method for detecting L. monocytogenes has strong specificity and high sensitivity, which is easy to operate, and can be performed under normal temperature without expensive equipment. It is suitable for field detection and application in the basic laboratory. |
| first_indexed | 2024-04-11T04:47:36Z |
| format | Article |
| id | doaj.art-41b1107fa7c646979c9782ef820b582a |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-04-11T04:47:36Z |
| publishDate | 2021-05-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-41b1107fa7c646979c9782ef820b582a2022-12-27T09:23:07ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562021-05-01330327427810.13590/j.cjfh.2021.03.0061004-8456(2021)03-0274-05Development of recombinase polymerase amplification detection method for Listeria monocytogenes in foodHE Jie0ZHU Chao1HUANG Shanshan2WU Qiaowei3SUN Ping4LAO Huajun5Technology Center of Ningbo Customs, Zhejiang Ningbo 315192, ChinaTechnology Center of Ningbo Customs, Zhejiang Ningbo 315192, ChinaTechnology Center of Ningbo Customs, Zhejiang Ningbo 315192, ChinaTechnology Center of Ningbo Customs, Zhejiang Ningbo 315192, ChinaTechnology Center of Ningbo Customs, Zhejiang Ningbo 315192, ChinaTechnology Center of Ningbo Customs, Zhejiang Ningbo 315192, ChinaObjectiveTo establish a recombinase polymerase amplification (RPA) method for the detection of Listeria monocytogenes (L. monocytogenes) in food.MethodsBased on the hlyA gene of L. monocytogenes, a set of RPA primers was selected for constructing RPA test, and its specificity and sensitivity were then tested.ResultsThe RPA assay could be finished in 30 min at 37 ℃. The primers used in RPA were specific. Experiments confirmed a detection limit of DNA template as low as 0.5 ng/μL. L. monocytogenes in the artificially-contaminated meat could be detected at the limit of 104 CFU/2.5 g.ConclusionThe RPA method for detecting L. monocytogenes has strong specificity and high sensitivity, which is easy to operate, and can be performed under normal temperature without expensive equipment. It is suitable for field detection and application in the basic laboratory.http://www.zgspws.com/zgspwszz/article/abstract/20210306?st=article_issuelisteria monocytogenesrecombinase polymerase amplificationhlyadetection |
| spellingShingle | HE Jie ZHU Chao HUANG Shanshan WU Qiaowei SUN Ping LAO Huajun Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food Zhongguo shipin weisheng zazhi listeria monocytogenes recombinase polymerase amplification hlya detection |
| title | Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food |
| title_full | Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food |
| title_fullStr | Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food |
| title_full_unstemmed | Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food |
| title_short | Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food |
| title_sort | development of recombinase polymerase amplification detection method for listeria monocytogenes in food |
| topic | listeria monocytogenes recombinase polymerase amplification hlya detection |
| url | http://www.zgspws.com/zgspwszz/article/abstract/20210306?st=article_issue |
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