SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for Wineries
Considering that many <i>Saccharomyces cerevisiae</i> strains exist and that they have different fermentation capacities, the challenge is to select the yeast strain that generates the most interesting wine character and wine flavor for the winemaker. A method based on simple sequence re...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/6/4/101 |
| _version_ | 1797549807812739072 |
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| author | Friederike Rex Adeline Hirschler Maren Scharfenberger-Schmeer |
| author_facet | Friederike Rex Adeline Hirschler Maren Scharfenberger-Schmeer |
| author_sort | Friederike Rex |
| collection | DOAJ |
| description | Considering that many <i>Saccharomyces cerevisiae</i> strains exist and that they have different fermentation capacities, the challenge is to select the yeast strain that generates the most interesting wine character and wine flavor for the winemaker. A method based on simple sequence repeats (SSRs) markers, occurring in the yeast genome, was developed to differentiate the collected <i>S.</i><i>cerevisiae</i> strains. For the amplification of the polymorphic SSR markers performed by polymerase chain reaction (PCR), two primer sets showing different size products for different <i>S. cerevisiae</i> strains were designed. The PCR-method with gel electrophoresis was validated using capillary sequencing and then used as a service for winegrowers combined with a sensory analysis via napping. This approach can be used for the preservation of the yeast diversity associated with given terroirs and as an option for an increased safety of fermentations. The application of <i>S. cerevisiae</i> strains collected in spontaneous fermentations and used for fermentation sustains the initial character of the wine and ensures a secure fermentation at the same time. |
| first_indexed | 2024-03-10T15:19:37Z |
| format | Article |
| id | doaj.art-41b242e19abc4970aacd50487a655a58 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T15:19:37Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-41b242e19abc4970aacd50487a655a582023-11-20T18:35:20ZengMDPI AGFermentation2311-56372020-10-016410110.3390/fermentation6040101SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for WineriesFriederike Rex0Adeline Hirschler1Maren Scharfenberger-Schmeer2Institute for Viticulture and Oenology, Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Breitenweg 71, D-67435 Neustadt, GermanyInstitute for Viticulture and Oenology, Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Breitenweg 71, D-67435 Neustadt, GermanyInstitute for Viticulture and Oenology, Dienstleistungszentrum Ländlicher Raum (DLR) Rheinpfalz, Breitenweg 71, D-67435 Neustadt, GermanyConsidering that many <i>Saccharomyces cerevisiae</i> strains exist and that they have different fermentation capacities, the challenge is to select the yeast strain that generates the most interesting wine character and wine flavor for the winemaker. A method based on simple sequence repeats (SSRs) markers, occurring in the yeast genome, was developed to differentiate the collected <i>S.</i><i>cerevisiae</i> strains. For the amplification of the polymorphic SSR markers performed by polymerase chain reaction (PCR), two primer sets showing different size products for different <i>S. cerevisiae</i> strains were designed. The PCR-method with gel electrophoresis was validated using capillary sequencing and then used as a service for winegrowers combined with a sensory analysis via napping. This approach can be used for the preservation of the yeast diversity associated with given terroirs and as an option for an increased safety of fermentations. The application of <i>S. cerevisiae</i> strains collected in spontaneous fermentations and used for fermentation sustains the initial character of the wine and ensures a secure fermentation at the same time.https://www.mdpi.com/2311-5637/6/4/101simple sequence repeats markers<i>Saccharomyces cerevisiae</i>napping |
| spellingShingle | Friederike Rex Adeline Hirschler Maren Scharfenberger-Schmeer SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for Wineries Fermentation simple sequence repeats markers <i>Saccharomyces cerevisiae</i> napping |
| title | SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for Wineries |
| title_full | SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for Wineries |
| title_fullStr | SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for Wineries |
| title_full_unstemmed | SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for Wineries |
| title_short | SSR-Marker Analysis—A Method for <i>S. cerevisiae</i> Strain Characterization and Its Application for Wineries |
| title_sort | ssr marker analysis a method for i s cerevisiae i strain characterization and its application for wineries |
| topic | simple sequence repeats markers <i>Saccharomyces cerevisiae</i> napping |
| url | https://www.mdpi.com/2311-5637/6/4/101 |
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