Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19
Background: This study aimed to determine the involvement of carboxypeptidase A3 (CPA3) in developing lung damage in patients with COVID-19. Methods and Results: The study included samples of autopsy material from the lungs of patients who died as a result of severe COVID-19 (the main group [MG] an...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2023-12-01
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| Series: | International Journal of Biomedicine |
| Subjects: | |
| Online Access: | http://www.ijbm.org/articles/i52/ijbm_13(4)_oa11.pdf |
| _version_ | 1827591744325156864 |
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| author | Andrey V. Budnevsky Sergey N. Avdeev Evgeniy S. Ovsyannikov Nadezhda G. Alekseeva Victoria V. Shishkina Inessa A. Savushkina Inna M. Perveeva Sofia N. Feigelman Avag G. Kitoyan Valeria R. Drobysheva |
| author_facet | Andrey V. Budnevsky Sergey N. Avdeev Evgeniy S. Ovsyannikov Nadezhda G. Alekseeva Victoria V. Shishkina Inessa A. Savushkina Inna M. Perveeva Sofia N. Feigelman Avag G. Kitoyan Valeria R. Drobysheva |
| author_sort | Andrey V. Budnevsky |
| collection | DOAJ |
| description | Background: This study aimed to determine the involvement of carboxypeptidase A3 (CPA3) in developing lung damage in patients with COVID-19.
Methods and Results: The study included samples of autopsy material from the lungs of patients who died as a result of severe COVID-19 (the main group [MG] and persons who died from external causes (the control group [CG]). Immunohistochemical staining for CPA3 was carried out. A quantitative study of CPA3-positive mast cells (MCs) and the degree of their degranulation was carried out using a ×40 objective lens with an analysis of ≥50 fields of view with further conversion to 1 mm².
Significant representation of CPA3-positive MCs per 1 mm2 of CPA3-positive MCs, CPA3-positive MCs with signs of degranulation (SD), and co-adjacent MCs was found in the MG compared to the CG (P=0.01 in all cases). In the main group, positive correlations were identified between the total number of CPA3-positive MCs, CPA3-positive MCs with SD and the blood hemoglobin level shortly before death (r=0.491 [P=0.008] and r=0.521 [P=0.004], respectively). Co-adjacent CPA3-positive MCs were negatively correlated with blood eosinophils at the beginning of hospitalization (r=-0.420 [P=0.023]). Also, the number of separately lying, CPA3-positive MCs negatively correlated with the blood monocyte shortly before death (r=-0.384 [P=0.044]). A positive correlation was established between the total number of CPA3-positive MCs, CPA3-positive MCs with SD, and adjacent CPA3-positive MCs with total blood protein in patients at the beginning of hospitalization (r=0.431 [P=0.020], r=0.449 [P=0.015] and r=0.456 [P=0.013], respectively). In addition, the study demonstrated a positive correlation between CPA3-positive MCs with SD and the total number of CPA3-positive MCs with blood aPTT levels (r=0.304 [P=0.045] and r=0.375 [P=0.045], respectively). A negative correlation was also found between the total number of CPA3-positive MCs and the blood INR level (r=-0.812 [P=0.050]). Finally, in patients at the beginning of hospitalization, a negative correlation was found between CPA3-positive MCs with SD, CPA3-positive MCs without SD, separately located CPA3-positive MCs, adjacent CPA3-positive MCs, and the total number of CPA3-positive MCs with blood amylase (r=-0.550 [P=0.002], r=-0.452 [P=0.045], r=-0.485 [P=0.030], r=-0.622 [P=0.008], and r=-0.590 [P=0.006], respectively).
Conclusion: Our study identifies the potential involvement of CPA3 in the pathogenesis of severe COVID-19. However, many aspects of its participation remain unclear and require further study. |
| first_indexed | 2024-03-09T01:37:01Z |
| format | Article |
| id | doaj.art-41b281c2cd7c48cbbdefd201ac6d7eef |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-03-09T01:37:01Z |
| publishDate | 2023-12-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-41b281c2cd7c48cbbdefd201ac6d7eef2023-12-09T05:17:25ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292023-12-0113430130510.21103/Article13(4)_OA11Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19Andrey V. Budnevsky0Sergey N. Avdeev1Evgeniy S. Ovsyannikov2Nadezhda G. Alekseeva3Victoria V. Shishkina4Inessa A. Savushkina5Inna M. Perveeva6Sofia N. Feigelman7Avag G. Kitoyan8Valeria R. Drobysheva9Voronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaI. M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaVoronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaVoronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaVoronezh State Medical University named after N.N. Burdenko, Voronezh, Russia; Research Institute of Experimental Biology and Medicine, Voronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaVoronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaVoronezh Regional Clinical Hospital No. 1, Voronezh, RussiaVoronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaVoronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaVoronezh State Medical University named after N.N. Burdenko, Voronezh, RussiaBackground: This study aimed to determine the involvement of carboxypeptidase A3 (CPA3) in developing lung damage in patients with COVID-19. Methods and Results: The study included samples of autopsy material from the lungs of patients who died as a result of severe COVID-19 (the main group [MG] and persons who died from external causes (the control group [CG]). Immunohistochemical staining for CPA3 was carried out. A quantitative study of CPA3-positive mast cells (MCs) and the degree of their degranulation was carried out using a ×40 objective lens with an analysis of ≥50 fields of view with further conversion to 1 mm². Significant representation of CPA3-positive MCs per 1 mm2 of CPA3-positive MCs, CPA3-positive MCs with signs of degranulation (SD), and co-adjacent MCs was found in the MG compared to the CG (P=0.01 in all cases). In the main group, positive correlations were identified between the total number of CPA3-positive MCs, CPA3-positive MCs with SD and the blood hemoglobin level shortly before death (r=0.491 [P=0.008] and r=0.521 [P=0.004], respectively). Co-adjacent CPA3-positive MCs were negatively correlated with blood eosinophils at the beginning of hospitalization (r=-0.420 [P=0.023]). Also, the number of separately lying, CPA3-positive MCs negatively correlated with the blood monocyte shortly before death (r=-0.384 [P=0.044]). A positive correlation was established between the total number of CPA3-positive MCs, CPA3-positive MCs with SD, and adjacent CPA3-positive MCs with total blood protein in patients at the beginning of hospitalization (r=0.431 [P=0.020], r=0.449 [P=0.015] and r=0.456 [P=0.013], respectively). In addition, the study demonstrated a positive correlation between CPA3-positive MCs with SD and the total number of CPA3-positive MCs with blood aPTT levels (r=0.304 [P=0.045] and r=0.375 [P=0.045], respectively). A negative correlation was also found between the total number of CPA3-positive MCs and the blood INR level (r=-0.812 [P=0.050]). Finally, in patients at the beginning of hospitalization, a negative correlation was found between CPA3-positive MCs with SD, CPA3-positive MCs without SD, separately located CPA3-positive MCs, adjacent CPA3-positive MCs, and the total number of CPA3-positive MCs with blood amylase (r=-0.550 [P=0.002], r=-0.452 [P=0.045], r=-0.485 [P=0.030], r=-0.622 [P=0.008], and r=-0.590 [P=0.006], respectively). Conclusion: Our study identifies the potential involvement of CPA3 in the pathogenesis of severe COVID-19. However, many aspects of its participation remain unclear and require further study.http://www.ijbm.org/articles/i52/ijbm_13(4)_oa11.pdfcovid-19carboxypeptidase a3mast cells |
| spellingShingle | Andrey V. Budnevsky Sergey N. Avdeev Evgeniy S. Ovsyannikov Nadezhda G. Alekseeva Victoria V. Shishkina Inessa A. Savushkina Inna M. Perveeva Sofia N. Feigelman Avag G. Kitoyan Valeria R. Drobysheva Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19 International Journal of Biomedicine covid-19 carboxypeptidase a3 mast cells |
| title | Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19 |
| title_full | Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19 |
| title_fullStr | Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19 |
| title_full_unstemmed | Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19 |
| title_short | Some Aspects of Mast Cells Carboxypeptidase A3 Participation in the Pathogenesis of COVID-19 |
| title_sort | some aspects of mast cells carboxypeptidase a3 participation in the pathogenesis of covid 19 |
| topic | covid-19 carboxypeptidase a3 mast cells |
| url | http://www.ijbm.org/articles/i52/ijbm_13(4)_oa11.pdf |
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