DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR
The study of detection effectiveness of sulfate-reducing bacteria (SRB) in the samples from various ecotopes by microbiological (cultural by serial dilutions) and molecular biological (by real-time PCR) methods with designed test-systems lyophilized on the silicon microchip was performed. The develo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-04-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/2_2020/abdulina_2_2020.pdf |
| Summary: | The study of detection effectiveness of sulfate-reducing bacteria (SRB) in the samples from various ecotopes by microbiological (cultural by serial dilutions) and molecular biological (by real-time PCR) methods with designed test-systems lyophilized on the silicon microchip was performed. The developed DSRM and SRB2 test-systems for detection of the functional gene dsrA presence, encoding one of the key enzyme of dissimilatory sulphate-reduction pathway — dissimilatory sulfite reductase were used. It was found that the minimal determined SRB titres in water samples were 104 cells/ml and in soil samples they were 102–105 cells/g of absolutely dry soil. In natural and man-caused samples, the amount of SRB detected by the microbiological method was correlated with calculated values determined by the molecular biological method, Pearson’s indexes were r = 0.41–0.69 (k = 11, P ≤ 0.01–0.05). Thus, real-time PCR assay with designed test systems lyophilized on the silicon microchips is a high-quality and rapid method for the detection of SRB in various natural and man-caused ecotopes. |
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| ISSN: | 2410-7751 2410-776X |