DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR
The study of detection effectiveness of sulfate-reducing bacteria (SRB) in the samples from various ecotopes by microbiological (cultural by serial dilutions) and molecular biological (by real-time PCR) methods with designed test-systems lyophilized on the silicon microchip was performed. The develo...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2020-04-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/2_2020/abdulina_2_2020.pdf |
| _version_ | 1797722690445901824 |
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| author | D. R. Abdulina G. O Iutynska A. I. Aniskina M. M. Nikitin |
| author_facet | D. R. Abdulina G. O Iutynska A. I. Aniskina M. M. Nikitin |
| author_sort | D. R. Abdulina |
| collection | DOAJ |
| description | The study of detection effectiveness of sulfate-reducing bacteria (SRB) in the samples from various ecotopes by microbiological (cultural by serial dilutions) and molecular biological (by real-time PCR) methods with designed test-systems lyophilized on the silicon microchip was performed. The developed DSRM and SRB2 test-systems for detection of the functional gene dsrA presence, encoding one of the key enzyme of dissimilatory sulphate-reduction pathway — dissimilatory sulfite reductase were used. It was found that the minimal determined SRB titres in water samples were 104 cells/ml and in soil samples they were 102–105 cells/g of absolutely dry soil. In natural and man-caused samples, the amount of SRB detected by the microbiological method was correlated with calculated values determined by the molecular biological method, Pearson’s indexes were r = 0.41–0.69 (k = 11, P ≤ 0.01–0.05). Thus, real-time PCR assay with designed test systems lyophilized on the silicon microchips is a high-quality and rapid method for the detection of SRB in various natural and man-caused ecotopes. |
| first_indexed | 2024-03-12T09:52:05Z |
| format | Article |
| id | doaj.art-41b55223046d429e8125b4d1db2edfb1 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T09:52:05Z |
| publishDate | 2020-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-41b55223046d429e8125b4d1db2edfb12023-09-02T12:26:22ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2020-04-011323847doi.org/10.15407/biotech13.02.038DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCRD. R. Abdulina0 G. O Iutynska1A. I. Aniskina2 M. M. Nikitin3D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, KyivD.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, KyivGenBit LLC, Moscow, RussiaGenBit LLC, Moscow, RussiaThe study of detection effectiveness of sulfate-reducing bacteria (SRB) in the samples from various ecotopes by microbiological (cultural by serial dilutions) and molecular biological (by real-time PCR) methods with designed test-systems lyophilized on the silicon microchip was performed. The developed DSRM and SRB2 test-systems for detection of the functional gene dsrA presence, encoding one of the key enzyme of dissimilatory sulphate-reduction pathway — dissimilatory sulfite reductase were used. It was found that the minimal determined SRB titres in water samples were 104 cells/ml and in soil samples they were 102–105 cells/g of absolutely dry soil. In natural and man-caused samples, the amount of SRB detected by the microbiological method was correlated with calculated values determined by the molecular biological method, Pearson’s indexes were r = 0.41–0.69 (k = 11, P ≤ 0.01–0.05). Thus, real-time PCR assay with designed test systems lyophilized on the silicon microchips is a high-quality and rapid method for the detection of SRB in various natural and man-caused ecotopes.http://biotechnology.kiev.ua/images/storage/2_2020/abdulina_2_2020.pdfdsra genetest-systemssulfate-reducing bacteriabiocorrosionreal-time pcr. |
| spellingShingle | D. R. Abdulina G. O Iutynska A. I. Aniskina M. M. Nikitin DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR Biotechnologia Acta dsra gene test-systems sulfate-reducing bacteria biocorrosion real-time pcr. |
| title | DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR |
| title_full | DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR |
| title_fullStr | DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR |
| title_full_unstemmed | DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR |
| title_short | DETECTION OF SULFATE-REDUCING BACTERIA FROM VARIOUS ECOTOPES BY REAL-TIME PCR |
| title_sort | detection of sulfate reducing bacteria from various ecotopes by real time pcr |
| topic | dsra gene test-systems sulfate-reducing bacteria biocorrosion real-time pcr. |
| url | http://biotechnology.kiev.ua/images/storage/2_2020/abdulina_2_2020.pdf |
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