Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography
Age-related cataracts are one of the most prevalent causes of visual impairment around the world. Understanding the mechanisms of cataract development and progression is essential to enable early clinical diagnosis and treatment to preserve visual acuity. Reductive chemicals are potential medicines...
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MDPI AG
2021-06-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/8/6/207 |
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| author | Hongqiu Zhang Manmohan Singh Achuth Nair Kirill V. Larin Salavat R. Aglyamov |
| author_facet | Hongqiu Zhang Manmohan Singh Achuth Nair Kirill V. Larin Salavat R. Aglyamov |
| author_sort | Hongqiu Zhang |
| collection | DOAJ |
| description | Age-related cataracts are one of the most prevalent causes of visual impairment around the world. Understanding the mechanisms of cataract development and progression is essential to enable early clinical diagnosis and treatment to preserve visual acuity. Reductive chemicals are potential medicines effective on cataract treatment. In this work, we investigated the cataract-induced oxidative damage in the crystalline lens and a kind of reductant, α-lipoic acid (ALA), ability to reduce the damage. We created oxidative environment to investigate the relationship between the progression of oxidative cataract and lenticular biomechanical properties measured by dynamic optical coherence elastography in porcine crystalline lenses ex vivo. The efficacy of ALA to minimize the stiffening of the lens was also quantified. The results showed a significant increase in Young’s modulus of the lens due to the formation of the oxidative cataract. We found a statistically significant difference between Young’s modulus of the lenses stored in phosphate-buffered saline and ALA solution after incubation in H<sub>2</sub>O<sub>2</sub> solution for 3 h (43.0 ± 9.0 kPa versus 20.7 ± 3.5 kPa, respectively). These results show that the lens stiffness increases during oxidative cataract formation, and ALA has the potential to reverse stiffening of the lens caused by oxidative damage. |
| first_indexed | 2024-03-10T10:36:59Z |
| format | Article |
| id | doaj.art-41b605bcd0984fde9f1fdedac3d301a0 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-10T10:36:59Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-41b605bcd0984fde9f1fdedac3d301a02023-11-21T23:12:21ZengMDPI AGPhotonics2304-67322021-06-018620710.3390/photonics8060207Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence ElastographyHongqiu Zhang0Manmohan Singh1Achuth Nair2Kirill V. Larin3Salavat R. Aglyamov4Department of Biomedical Engineering, University of Houston, 3517 Cullen Blvd., Room 2027, Houston, TX 77204, USADepartment of Biomedical Engineering, University of Houston, 3517 Cullen Blvd., Room 2027, Houston, TX 77204, USADepartment of Biomedical Engineering, University of Houston, 3517 Cullen Blvd., Room 2027, Houston, TX 77204, USADepartment of Biomedical Engineering, University of Houston, 3517 Cullen Blvd., Room 2027, Houston, TX 77204, USADepartment of Mechanical Engineering, University of Houston, 4726 Calhoun Rd., Room N207., Houston, TX 77204, USAAge-related cataracts are one of the most prevalent causes of visual impairment around the world. Understanding the mechanisms of cataract development and progression is essential to enable early clinical diagnosis and treatment to preserve visual acuity. Reductive chemicals are potential medicines effective on cataract treatment. In this work, we investigated the cataract-induced oxidative damage in the crystalline lens and a kind of reductant, α-lipoic acid (ALA), ability to reduce the damage. We created oxidative environment to investigate the relationship between the progression of oxidative cataract and lenticular biomechanical properties measured by dynamic optical coherence elastography in porcine crystalline lenses ex vivo. The efficacy of ALA to minimize the stiffening of the lens was also quantified. The results showed a significant increase in Young’s modulus of the lens due to the formation of the oxidative cataract. We found a statistically significant difference between Young’s modulus of the lenses stored in phosphate-buffered saline and ALA solution after incubation in H<sub>2</sub>O<sub>2</sub> solution for 3 h (43.0 ± 9.0 kPa versus 20.7 ± 3.5 kPa, respectively). These results show that the lens stiffness increases during oxidative cataract formation, and ALA has the potential to reverse stiffening of the lens caused by oxidative damage.https://www.mdpi.com/2304-6732/8/6/207crystalline lensoptical coherence elastographyelasticityalpha-lipoic acidoxidationcataract |
| spellingShingle | Hongqiu Zhang Manmohan Singh Achuth Nair Kirill V. Larin Salavat R. Aglyamov Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography Photonics crystalline lens optical coherence elastography elasticity alpha-lipoic acid oxidation cataract |
| title | Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography |
| title_full | Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography |
| title_fullStr | Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography |
| title_full_unstemmed | Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography |
| title_short | Elasticity Changes in the Crystalline Lens during Oxidative Damage and the Antioxidant Effect of Alpha-Lipoic Acid Measured by Optical Coherence Elastography |
| title_sort | elasticity changes in the crystalline lens during oxidative damage and the antioxidant effect of alpha lipoic acid measured by optical coherence elastography |
| topic | crystalline lens optical coherence elastography elasticity alpha-lipoic acid oxidation cataract |
| url | https://www.mdpi.com/2304-6732/8/6/207 |
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