Mesenchymal Stem Cell Isolation from the Removed Medium of Rat’s Bone Marrow Primary Culture and their Differentiation into Skeletal Cell Lineages
Objective: In all protocols for isolation of mesenchymal stem cells (MSCs), a fewdays after culture initiation, the medium were discarded along with its contentsof non-adherent cells and the adherent cell population kept and expanded asMSCs population. In the present study, attempt was made to expan...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2008-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_5273346443332254464623223336564224375723slami.pdf |
| Summary: | Objective: In all protocols for isolation of mesenchymal stem cells (MSCs), a fewdays after culture initiation, the medium were discarded along with its contentsof non-adherent cells and the adherent cell population kept and expanded asMSCs population. In the present study, attempt was made to expand the cellssuspended in removed medium of primary culture and compare them with theadherent cell population.Materials and Methods: Four days after rat’s bone marrow culture initiation,medium of the culture was collected and its suspended cells were cultureexpandedin parallel with adherent cells till passage 3. During the cultureperiod, the cells from either group were statistically compared with respect ofthe time required for cell confluency (the stage in which cells cover the entiresurfaces) as an index of growth rate. At the end, the cells from both cultureswere evaluated in terms of their differentiation potential.Results: The primary culture of the cells from removed medium contained largecolonies of spindle-shaped cells that reached into confluency after 5.36±0.5days, while those from the adherent population possessed small coloniesreaching into confluency in 8.09±0.70 days. According to the results, at allstudied passages, the cells of removed medium were significantly (p<0.05)achieved confluency in shorter time than the adherent population. Moreover,the cells from either culture could easily differentiate into bone, cartilage andadipose cells.Conclusions: It seems that some cells from removed medium, usuallydiscarded in medium substitution, are MSCs possessing more growth rate thanthe primarily adherent cell population. |
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| ISSN: | 2228-5806 2228-5814 |