Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated Boars
<p/> <p>The present study was designed to evaluate the effects of synthetic ACTH (1–24, tetracosactid) and porcine CRH on the plasma levels of cortisol and PGF<sub>2α </sub>metabolite in cycling gilts (n = 3) and castrated boars (n = 3). The experiments were designed as cross...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2005-12-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | http://www.actavetscand.com/content/46/4/249 |
| _version_ | 1818520520732180480 |
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| author | Kindahl H Mwanza AM Madej A Einarsson S |
| author_facet | Kindahl H Mwanza AM Madej A Einarsson S |
| author_sort | Kindahl H |
| collection | DOAJ |
| description | <p/> <p>The present study was designed to evaluate the effects of synthetic ACTH (1–24, tetracosactid) and porcine CRH on the plasma levels of cortisol and PGF<sub>2α </sub>metabolite in cycling gilts (n = 3) and castrated boars (n = 3). The experiments were designed as crossover studies for each gender separately. Each animal received, during three consecutive days; 1) ACTH (Synacthen<sup>® </sup>Depot) at a dose of 10 μg/kg body weight in 5 ml physiological saline, 2) porcine CRH at a dose 0.6 μg/kg body weight in 5 ml physiological saline or 3) physiological saline (5 ml). The test substances were administered via an indwelling jugular cannula in randomized order according to a Latin square. The administration of ACTH to cycling gilts resulted in concomitant elevations of cortisol and PGF<sub>2α </sub>metabolite with peak levels reached at 70.0 ± 10.0 and 33.3 ± 6.7 min, respectively. Similarly, the administration of ACTH to castrated boars resulted in concomitant elevation of cortisol and PGF<sub>2α </sub>metabolite with peak levels reached at 60.0 ± 0.0 and 20.0 ± 0.0 min, respectively. Cortisol peaked at 20 min after administration of CRH in both cycling gilts and castrated boars with maximum levels of 149.3 ± 16.5 nmol/1 and 138.3 ± 10.1 nmol/1, respectively. It can be concluded that administration of synthetic ACTH (tetracosactid) to pigs caused a concomitant elevation of cortisol and PGF<sub>2α </sub>metabolite levels in both cycling gilts as well as castrated boars. The administration of CRH to pigs resulted in an elevation of cortisol levels in both cycling gilts and castrated boars. Conversely, PGF<sub>2α </sub>metabolite levels were not influenced by the administration of CRH either in cycling gilts or in castrated boars.</p> |
| first_indexed | 2024-12-11T01:38:37Z |
| format | Article |
| id | doaj.art-41ba2936754047ce839fafa985d0d3dd |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-12-11T01:38:37Z |
| publishDate | 2005-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-41ba2936754047ce839fafa985d0d3dd2022-12-22T01:25:07ZengBMCActa Veterinaria Scandinavica1751-01472005-12-0146424925610.1186/1751-0147-46-249Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated BoarsKindahl HMwanza AMMadej AEinarsson S<p/> <p>The present study was designed to evaluate the effects of synthetic ACTH (1–24, tetracosactid) and porcine CRH on the plasma levels of cortisol and PGF<sub>2α </sub>metabolite in cycling gilts (n = 3) and castrated boars (n = 3). The experiments were designed as crossover studies for each gender separately. Each animal received, during three consecutive days; 1) ACTH (Synacthen<sup>® </sup>Depot) at a dose of 10 μg/kg body weight in 5 ml physiological saline, 2) porcine CRH at a dose 0.6 μg/kg body weight in 5 ml physiological saline or 3) physiological saline (5 ml). The test substances were administered via an indwelling jugular cannula in randomized order according to a Latin square. The administration of ACTH to cycling gilts resulted in concomitant elevations of cortisol and PGF<sub>2α </sub>metabolite with peak levels reached at 70.0 ± 10.0 and 33.3 ± 6.7 min, respectively. Similarly, the administration of ACTH to castrated boars resulted in concomitant elevation of cortisol and PGF<sub>2α </sub>metabolite with peak levels reached at 60.0 ± 0.0 and 20.0 ± 0.0 min, respectively. Cortisol peaked at 20 min after administration of CRH in both cycling gilts and castrated boars with maximum levels of 149.3 ± 16.5 nmol/1 and 138.3 ± 10.1 nmol/1, respectively. It can be concluded that administration of synthetic ACTH (tetracosactid) to pigs caused a concomitant elevation of cortisol and PGF<sub>2α </sub>metabolite levels in both cycling gilts as well as castrated boars. The administration of CRH to pigs resulted in an elevation of cortisol levels in both cycling gilts and castrated boars. Conversely, PGF<sub>2α </sub>metabolite levels were not influenced by the administration of CRH either in cycling gilts or in castrated boars.</p>http://www.actavetscand.com/content/46/4/249ACTHCRHcortisolPGF<sub>2α </sub>metabolitegiltscastrated boars |
| spellingShingle | Kindahl H Mwanza AM Madej A Einarsson S Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated Boars Acta Veterinaria Scandinavica ACTH CRH cortisol PGF<sub>2α </sub>metabolite gilts castrated boars |
| title | Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated Boars |
| title_full | Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated Boars |
| title_fullStr | Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated Boars |
| title_full_unstemmed | Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated Boars |
| title_short | Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F<sub>2α </sub>Metabolite in Cycling Gilts and Castrated Boars |
| title_sort | effect of acth and crh on plasma levels of cortisol and prostaglandin f sub 2α sub metabolite in cycling gilts and castrated boars |
| topic | ACTH CRH cortisol PGF<sub>2α </sub>metabolite gilts castrated boars |
| url | http://www.actavetscand.com/content/46/4/249 |
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