C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickens
Abstract Matrix metalloproteinases (MMPs) play an important role in intestinal extracellular matrix homeostasis. An overexpression of MMPs results in tissue destruction and local inflammation and has been associated with multiple inflammatory diseases. These host proteases might also be important in...
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | Veterinary Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13567-020-00825-6 |
| _version_ | 1818273629348036608 |
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| author | Lore Van Damme Natasja Cox Chana Callens Freddy Haesebrouck Michelle Dargatz Richard Ducatelle Filip Van Immerseel Evy Goossens |
| author_facet | Lore Van Damme Natasja Cox Chana Callens Freddy Haesebrouck Michelle Dargatz Richard Ducatelle Filip Van Immerseel Evy Goossens |
| author_sort | Lore Van Damme |
| collection | DOAJ |
| description | Abstract Matrix metalloproteinases (MMPs) play an important role in intestinal extracellular matrix homeostasis. An overexpression of MMPs results in tissue destruction and local inflammation and has been associated with multiple inflammatory diseases. These host proteases might also be important in tissue damage caused by infectious agents, such as in intestinal damage in Clostridium perfringens-induced avian necrotic enteritis (NE). The aim of the present study was to elucidate the effect of a C. perfringens infection on the MMP activity in the small intestine of birds with a pre-disposing coccidial infection to obtain a more thorough understanding of the pathogenesis of NE. For this purpose, the gelatinolytic activity present in jejunal tissue of Eimeria infected birds which were challenged with either a pathogenic C. perfringens type G strain or a commensal C. perfringens type A strain was analyzed using substrate zymography. The results show that infection of broilers with Eimeria and different C. perfringens strains, independent of their pathogenicity, decreases the expression of a 40–45 kDa host collagenase in the jejunum, as compared to the expression in Eimeria-infected control birds. It was also shown that the expression of 2 MMPs with molecular weights of approximately 50–60 and 60–70 kDa was significantly lower in necrotic tissue as compared to the activity in macroscopically healthy tissue adjacent to the lesion. These results indicate that host collagenases are not elicited by the C. perfringens infection for permeabilizing the host mucosa to allow penetration of the NetB toxin in Eimeria infected broilers. |
| first_indexed | 2024-12-12T22:01:00Z |
| format | Article |
| id | doaj.art-41baf59ab8ff4b65a137a7b1de0fb8b5 |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-12-12T22:01:00Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-41baf59ab8ff4b65a137a7b1de0fb8b52022-12-22T00:10:31ZengBMCVeterinary Research1297-97162020-08-015111810.1186/s13567-020-00825-6C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickensLore Van Damme0Natasja Cox1Chana Callens2Freddy Haesebrouck3Michelle Dargatz4Richard Ducatelle5Filip Van Immerseel6Evy Goossens7Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDivision Nutrition & Care-Animal Nutrition, Evonik Operations GmbHDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityDepartment of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent UniversityAbstract Matrix metalloproteinases (MMPs) play an important role in intestinal extracellular matrix homeostasis. An overexpression of MMPs results in tissue destruction and local inflammation and has been associated with multiple inflammatory diseases. These host proteases might also be important in tissue damage caused by infectious agents, such as in intestinal damage in Clostridium perfringens-induced avian necrotic enteritis (NE). The aim of the present study was to elucidate the effect of a C. perfringens infection on the MMP activity in the small intestine of birds with a pre-disposing coccidial infection to obtain a more thorough understanding of the pathogenesis of NE. For this purpose, the gelatinolytic activity present in jejunal tissue of Eimeria infected birds which were challenged with either a pathogenic C. perfringens type G strain or a commensal C. perfringens type A strain was analyzed using substrate zymography. The results show that infection of broilers with Eimeria and different C. perfringens strains, independent of their pathogenicity, decreases the expression of a 40–45 kDa host collagenase in the jejunum, as compared to the expression in Eimeria-infected control birds. It was also shown that the expression of 2 MMPs with molecular weights of approximately 50–60 and 60–70 kDa was significantly lower in necrotic tissue as compared to the activity in macroscopically healthy tissue adjacent to the lesion. These results indicate that host collagenases are not elicited by the C. perfringens infection for permeabilizing the host mucosa to allow penetration of the NetB toxin in Eimeria infected broilers.http://link.springer.com/article/10.1186/s13567-020-00825-6broilerClostridium perfringensEimeriagutmatrix metalloproteinasesnecrotic enteritis |
| spellingShingle | Lore Van Damme Natasja Cox Chana Callens Freddy Haesebrouck Michelle Dargatz Richard Ducatelle Filip Van Immerseel Evy Goossens C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickens Veterinary Research broiler Clostridium perfringens Eimeria gut matrix metalloproteinases necrotic enteritis |
| title | C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickens |
| title_full | C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickens |
| title_fullStr | C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickens |
| title_full_unstemmed | C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickens |
| title_short | C. perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of Eimeria-infected broiler chickens |
| title_sort | c perfringens challenge reduces matrix metalloproteinase activity in the jejunal mucosa of eimeria infected broiler chickens |
| topic | broiler Clostridium perfringens Eimeria gut matrix metalloproteinases necrotic enteritis |
| url | http://link.springer.com/article/10.1186/s13567-020-00825-6 |
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