Diagnostic dilemma: application of real-time PCR assays for the detection of Dientamoeba fragilis in medical and veterinary specimens

Abstract Background Real-time PCR (qPCR) diagnostics developed for use in human clinical settings have been implemented to identify new animal hosts of the gastrointestinal protozoan Dientamoeba fragilis. The gut microbiome varies between species; unrecognised cross-reactivity could occur when apply...

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Bibliographic Details
Main Authors: Luke M. Hall, John T. Ellis, Damien J. Stark
Format: Article
Language:English
Published: BMC 2025-03-01
Series:Parasites & Vectors
Subjects:
Online Access:https://doi.org/10.1186/s13071-025-06730-1