The Response of HeLa Cells to Fluorescent NanoDiamond Uptake
Fluorescent nanodiamonds are promising probes for nanoscale magnetic resonance measurements. Their physical properties predict them to have particularly useful applications in intracellular analysis. Before using them in intracellular experiments however, it should be clear whether diamond particles...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-01-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/18/2/355 |
| _version_ | 1828393272902418432 |
|---|---|
| author | Simon R. Hemelaar Babujhi Saspaanithy Severin R. M. L’Hommelet Felipe P. Perona Martinez Kiran J. van der Laan Romana Schirhagl |
| author_facet | Simon R. Hemelaar Babujhi Saspaanithy Severin R. M. L’Hommelet Felipe P. Perona Martinez Kiran J. van der Laan Romana Schirhagl |
| author_sort | Simon R. Hemelaar |
| collection | DOAJ |
| description | Fluorescent nanodiamonds are promising probes for nanoscale magnetic resonance measurements. Their physical properties predict them to have particularly useful applications in intracellular analysis. Before using them in intracellular experiments however, it should be clear whether diamond particles influence cell biology. While cytotoxicity has already been ruled out in previous studies, we consider the non-fatal influence of fluorescent nanodiamonds on the formation of reactive oxygen species (an important stress indicator and potential target for intracellular sensing) for the first time. We investigated the influence of different sizes, shapes and concentrations of nanodiamonds on the genetic and protein level involved in oxidative stress-related pathways of the HeLa cell, an important model cell line in research. The changes in viability of the cells and the difference in intracellular levels of free radicals, after diamond uptake, are surprisingly small. At lower diamond concentrations, the cellular metabolism cannot be distinguished from that of untreated cells. This research supports the claims of non-toxicity and includes less obvious non-fatal responses. Finally, we give a handhold concerning the diamond concentration and size to use for non-toxic, intracellular measurements in favour of (cancer) research in HeLa cells. |
| first_indexed | 2024-12-10T07:36:10Z |
| format | Article |
| id | doaj.art-41bd36a4ec2c46cfa55c96af42b6042f |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T07:36:10Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-41bd36a4ec2c46cfa55c96af42b6042f2022-12-22T01:57:25ZengMDPI AGSensors1424-82202018-01-0118235510.3390/s18020355s18020355The Response of HeLa Cells to Fluorescent NanoDiamond UptakeSimon R. Hemelaar0Babujhi Saspaanithy1Severin R. M. L’Hommelet2Felipe P. Perona Martinez3Kiran J. van der Laan4Romana Schirhagl5Department of Biomedical Engineering, University of Groningen, 9713 AV Groningen, The NetherlandsDepartment of Biomedical Engineering, University of Groningen, 9713 AV Groningen, The NetherlandsDepartment of Biomedical Engineering, University of Groningen, 9713 AV Groningen, The NetherlandsDepartment of Biomedical Engineering, University of Groningen, 9713 AV Groningen, The NetherlandsDepartment of Biomedical Engineering, University of Groningen, 9713 AV Groningen, The NetherlandsDepartment of Biomedical Engineering, University of Groningen, 9713 AV Groningen, The NetherlandsFluorescent nanodiamonds are promising probes for nanoscale magnetic resonance measurements. Their physical properties predict them to have particularly useful applications in intracellular analysis. Before using them in intracellular experiments however, it should be clear whether diamond particles influence cell biology. While cytotoxicity has already been ruled out in previous studies, we consider the non-fatal influence of fluorescent nanodiamonds on the formation of reactive oxygen species (an important stress indicator and potential target for intracellular sensing) for the first time. We investigated the influence of different sizes, shapes and concentrations of nanodiamonds on the genetic and protein level involved in oxidative stress-related pathways of the HeLa cell, an important model cell line in research. The changes in viability of the cells and the difference in intracellular levels of free radicals, after diamond uptake, are surprisingly small. At lower diamond concentrations, the cellular metabolism cannot be distinguished from that of untreated cells. This research supports the claims of non-toxicity and includes less obvious non-fatal responses. Finally, we give a handhold concerning the diamond concentration and size to use for non-toxic, intracellular measurements in favour of (cancer) research in HeLa cells.http://www.mdpi.com/1424-8220/18/2/355fluorescent nanodiamondsreactive oxygen speciescellular uptakebiocompatibility |
| spellingShingle | Simon R. Hemelaar Babujhi Saspaanithy Severin R. M. L’Hommelet Felipe P. Perona Martinez Kiran J. van der Laan Romana Schirhagl The Response of HeLa Cells to Fluorescent NanoDiamond Uptake Sensors fluorescent nanodiamonds reactive oxygen species cellular uptake biocompatibility |
| title | The Response of HeLa Cells to Fluorescent NanoDiamond Uptake |
| title_full | The Response of HeLa Cells to Fluorescent NanoDiamond Uptake |
| title_fullStr | The Response of HeLa Cells to Fluorescent NanoDiamond Uptake |
| title_full_unstemmed | The Response of HeLa Cells to Fluorescent NanoDiamond Uptake |
| title_short | The Response of HeLa Cells to Fluorescent NanoDiamond Uptake |
| title_sort | response of hela cells to fluorescent nanodiamond uptake |
| topic | fluorescent nanodiamonds reactive oxygen species cellular uptake biocompatibility |
| url | http://www.mdpi.com/1424-8220/18/2/355 |
| work_keys_str_mv | AT simonrhemelaar theresponseofhelacellstofluorescentnanodiamonduptake AT babujhisaspaanithy theresponseofhelacellstofluorescentnanodiamonduptake AT severinrmlhommelet theresponseofhelacellstofluorescentnanodiamonduptake AT felipepperonamartinez theresponseofhelacellstofluorescentnanodiamonduptake AT kiranjvanderlaan theresponseofhelacellstofluorescentnanodiamonduptake AT romanaschirhagl theresponseofhelacellstofluorescentnanodiamonduptake AT simonrhemelaar responseofhelacellstofluorescentnanodiamonduptake AT babujhisaspaanithy responseofhelacellstofluorescentnanodiamonduptake AT severinrmlhommelet responseofhelacellstofluorescentnanodiamonduptake AT felipepperonamartinez responseofhelacellstofluorescentnanodiamonduptake AT kiranjvanderlaan responseofhelacellstofluorescentnanodiamonduptake AT romanaschirhagl responseofhelacellstofluorescentnanodiamonduptake |