Recent Advancements in Tracking Bacterial Effector Protein Translocation
Bacteria-host interactions are characterized by the delivery of bacterial virulence factors, i.e., effectors, into host cells where they counteract host immunity and exploit host responses allowing bacterial survival and spreading. These effectors are translocated into host cells by means of dedicat...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/2/260 |
| _version_ | 1797477845670297600 |
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| author | Julie Braet Dominiek Catteeuw Petra Van Damme |
| author_facet | Julie Braet Dominiek Catteeuw Petra Van Damme |
| author_sort | Julie Braet |
| collection | DOAJ |
| description | Bacteria-host interactions are characterized by the delivery of bacterial virulence factors, i.e., effectors, into host cells where they counteract host immunity and exploit host responses allowing bacterial survival and spreading. These effectors are translocated into host cells by means of dedicated secretion systems such as the type 3 secretion system (T3SS). A comprehensive understanding of effector translocation in a spatio-temporal manner is of critical importance to gain insights into an effector’s mode of action. Various approaches have been developed to understand timing and order of effector translocation, quantities of translocated effectors and their subcellular localization upon translocation into host cells. Recently, the existing toolset has been expanded by newly developed state-of-the art methods to monitor bacterial effector translocation and dynamics. In this review, we elaborate on reported methods and discuss recent advances and shortcomings in this area of tracking bacterial effector translocation. |
| first_indexed | 2024-03-09T21:23:32Z |
| format | Article |
| id | doaj.art-41c057c5da9a45049b19fa05c3f8b0d9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T21:23:32Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-41c057c5da9a45049b19fa05c3f8b0d92023-11-23T21:13:20ZengMDPI AGMicroorganisms2076-26072022-01-0110226010.3390/microorganisms10020260Recent Advancements in Tracking Bacterial Effector Protein TranslocationJulie Braet0Dominiek Catteeuw1Petra Van Damme2iRIP Unit, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, BelgiumiRIP Unit, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, BelgiumiRIP Unit, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, BelgiumBacteria-host interactions are characterized by the delivery of bacterial virulence factors, i.e., effectors, into host cells where they counteract host immunity and exploit host responses allowing bacterial survival and spreading. These effectors are translocated into host cells by means of dedicated secretion systems such as the type 3 secretion system (T3SS). A comprehensive understanding of effector translocation in a spatio-temporal manner is of critical importance to gain insights into an effector’s mode of action. Various approaches have been developed to understand timing and order of effector translocation, quantities of translocated effectors and their subcellular localization upon translocation into host cells. Recently, the existing toolset has been expanded by newly developed state-of-the art methods to monitor bacterial effector translocation and dynamics. In this review, we elaborate on reported methods and discuss recent advances and shortcomings in this area of tracking bacterial effector translocation.https://www.mdpi.com/2076-2607/10/2/260effectorFASTgenetic code expansionlocalizationNanoLucself-labeling enzymes |
| spellingShingle | Julie Braet Dominiek Catteeuw Petra Van Damme Recent Advancements in Tracking Bacterial Effector Protein Translocation Microorganisms effector FAST genetic code expansion localization NanoLuc self-labeling enzymes |
| title | Recent Advancements in Tracking Bacterial Effector Protein Translocation |
| title_full | Recent Advancements in Tracking Bacterial Effector Protein Translocation |
| title_fullStr | Recent Advancements in Tracking Bacterial Effector Protein Translocation |
| title_full_unstemmed | Recent Advancements in Tracking Bacterial Effector Protein Translocation |
| title_short | Recent Advancements in Tracking Bacterial Effector Protein Translocation |
| title_sort | recent advancements in tracking bacterial effector protein translocation |
| topic | effector FAST genetic code expansion localization NanoLuc self-labeling enzymes |
| url | https://www.mdpi.com/2076-2607/10/2/260 |
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