Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study
<p>Abstract</p> <p>Background</p> <p>Epigenetic regulation is an important mechanism leading to cancer initiation and promotion. Histone acetylation by histone deacetylases (HDACs) represents an important part of it. The development of HDAC inhibitors has identified the...
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| Format: | Article |
| Language: | English |
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BMC
2011-10-01
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| Series: | BMC Cancer |
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| Online Access: | http://www.biomedcentral.com/1471-2407/11/463 |
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| author | Samartzis Nicolas Imesch Patrick Dedes Konstantin J Samartzis Eleftherios P Fedier André Fink Daniel Caduff Rosmarie Fehr Mathias K |
| author_facet | Samartzis Nicolas Imesch Patrick Dedes Konstantin J Samartzis Eleftherios P Fedier André Fink Daniel Caduff Rosmarie Fehr Mathias K |
| author_sort | Samartzis Nicolas |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Epigenetic regulation is an important mechanism leading to cancer initiation and promotion. Histone acetylation by histone deacetylases (HDACs) represents an important part of it. The development of HDAC inhibitors has identified the utility of HDACs as a therapeutic target. Little is known about the epigenetic regulation of vulvar intraepithelial neoplasia (VIN) and vulvar squamous cell cancer (VSCC). In this study, the expression of class I HDACs (HDAC 1, 2 and 3) was compared in a series of VIN and VSCC tissues.</p> <p>Methods</p> <p>A tissue micro array (TMA) with specimens from 106 patients with high-grade VIN and 59 patients with vulvar cancer was constructed. The expression of HDACs 1, 2 and 3 were analyzed with immunohistochemistry (IHC). The nuclear expression pattern was evaluated in terms of intensity and percentage of stained nuclei and was compared between vulvar preinvasive lesions and vulvar cancer.</p> <p>Results</p> <p>HDAC 2 expression was significantly higher in VIN than in VSCC (p < 0.001, Fisher's test). Also, 88.7% (n = 94/106) of VIN samples and only 54.5% (n = 31/57) of VSCC samples were scored at the maximum level. Conversely, HDAC 3 expression was significantly higher in VSCC (93%, 53/57) compared to VIN (73.6%, 78/106, p = 0.003), whereas only a small difference in the expression of HDAC 1 was found between these two entities of vulvar neoplasia.</p> <p>Conclusions</p> <p>These results suggest that epigenetic regulation plays a considerable role in the transformation of VIN to invasive vulvar neoplasia.</p> |
| first_indexed | 2024-12-21T21:11:26Z |
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| id | doaj.art-41c3fe09c27a4551b4ae5fc1bbbb4a9d |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-21T21:11:26Z |
| publishDate | 2011-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-41c3fe09c27a4551b4ae5fc1bbbb4a9d2022-12-21T18:50:07ZengBMCBMC Cancer1471-24072011-10-0111146310.1186/1471-2407-11-463Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray studySamartzis NicolasImesch PatrickDedes Konstantin JSamartzis Eleftherios PFedier AndréFink DanielCaduff RosmarieFehr Mathias K<p>Abstract</p> <p>Background</p> <p>Epigenetic regulation is an important mechanism leading to cancer initiation and promotion. Histone acetylation by histone deacetylases (HDACs) represents an important part of it. The development of HDAC inhibitors has identified the utility of HDACs as a therapeutic target. Little is known about the epigenetic regulation of vulvar intraepithelial neoplasia (VIN) and vulvar squamous cell cancer (VSCC). In this study, the expression of class I HDACs (HDAC 1, 2 and 3) was compared in a series of VIN and VSCC tissues.</p> <p>Methods</p> <p>A tissue micro array (TMA) with specimens from 106 patients with high-grade VIN and 59 patients with vulvar cancer was constructed. The expression of HDACs 1, 2 and 3 were analyzed with immunohistochemistry (IHC). The nuclear expression pattern was evaluated in terms of intensity and percentage of stained nuclei and was compared between vulvar preinvasive lesions and vulvar cancer.</p> <p>Results</p> <p>HDAC 2 expression was significantly higher in VIN than in VSCC (p < 0.001, Fisher's test). Also, 88.7% (n = 94/106) of VIN samples and only 54.5% (n = 31/57) of VSCC samples were scored at the maximum level. Conversely, HDAC 3 expression was significantly higher in VSCC (93%, 53/57) compared to VIN (73.6%, 78/106, p = 0.003), whereas only a small difference in the expression of HDAC 1 was found between these two entities of vulvar neoplasia.</p> <p>Conclusions</p> <p>These results suggest that epigenetic regulation plays a considerable role in the transformation of VIN to invasive vulvar neoplasia.</p>http://www.biomedcentral.com/1471-2407/11/463Histone deacetylaseepigeneticsvulvar intraepithelial neoplasiavulvar squamous cell cancertissue microarrayimmunohistochemistry |
| spellingShingle | Samartzis Nicolas Imesch Patrick Dedes Konstantin J Samartzis Eleftherios P Fedier André Fink Daniel Caduff Rosmarie Fehr Mathias K Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study BMC Cancer Histone deacetylase epigenetics vulvar intraepithelial neoplasia vulvar squamous cell cancer tissue microarray immunohistochemistry |
| title | Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study |
| title_full | Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study |
| title_fullStr | Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study |
| title_full_unstemmed | Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study |
| title_short | Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study |
| title_sort | expression pattern of class i histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer a tissue microarray study |
| topic | Histone deacetylase epigenetics vulvar intraepithelial neoplasia vulvar squamous cell cancer tissue microarray immunohistochemistry |
| url | http://www.biomedcentral.com/1471-2407/11/463 |
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