mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions
ABSTRACT Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei. In this mSphere of Influence article, he reflects on how the paper “Comprehensive identification of RNA-protein interactions in any organism using orthogonal organ...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019-10-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00600-19 |
| _version_ | 1818836620990742528 |
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| author | Calvin Tiengwe |
| author_facet | Calvin Tiengwe |
| author_sort | Calvin Tiengwe |
| collection | DOAJ |
| description | ABSTRACT Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei. In this mSphere of Influence article, he reflects on how the paper “Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)” by Queiroz et al. (Nat Biotechnol 37:169–178, 2019, https://doi.org/10.1038/s41587-018-0001-2) influenced his research by providing a tool to capture RNA-protein complexes on a global scale using acid guanidinium thiocyanate-phenol-chloroform (AGPC), an old method hitherto applied for RNA, DNA, or protein purification. |
| first_indexed | 2024-12-19T03:09:31Z |
| format | Article |
| id | doaj.art-41c4991d564f46c080f5efa01de5723e |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-19T03:09:31Z |
| publishDate | 2019-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-41c4991d564f46c080f5efa01de5723e2022-12-21T20:38:03ZengAmerican Society for MicrobiologymSphere2379-50422019-10-014510.1128/mSphere.00600-19mSphere of Influence: Modifying an Old Method To Study RNA-Protein InteractionsCalvin Tiengwe0Department of Life Sciences, Imperial College London, London, United KingdomABSTRACT Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei. In this mSphere of Influence article, he reflects on how the paper “Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)” by Queiroz et al. (Nat Biotechnol 37:169–178, 2019, https://doi.org/10.1038/s41587-018-0001-2) influenced his research by providing a tool to capture RNA-protein complexes on a global scale using acid guanidinium thiocyanate-phenol-chloroform (AGPC), an old method hitherto applied for RNA, DNA, or protein purification.https://journals.asm.org/doi/10.1128/mSphere.00600-19AGPCOOPSRNA binding proteins |
| spellingShingle | Calvin Tiengwe mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions mSphere AGPC OOPS RNA binding proteins |
| title | mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions |
| title_full | mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions |
| title_fullStr | mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions |
| title_full_unstemmed | mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions |
| title_short | mSphere of Influence: Modifying an Old Method To Study RNA-Protein Interactions |
| title_sort | msphere of influence modifying an old method to study rna protein interactions |
| topic | AGPC OOPS RNA binding proteins |
| url | https://journals.asm.org/doi/10.1128/mSphere.00600-19 |
| work_keys_str_mv | AT calvintiengwe msphereofinfluencemodifyinganoldmethodtostudyrnaproteininteractions |