| Summary: | Primordium formation is extremely important for yield of <i>Pleurotus tuoliensis</i>. However, the molecular mechanism underlying primordium formation is largely unknown. This study investigated the transcriptional properties during primordium formation of <i>P. tuoliensis</i> by comparing transcriptome. Clean reads were assembled into 57,075 transcripts and 6874 unigenes. A total of 1397 differentially expressed genes were identified (26 DEGs altered in all stages). GO and KEGG enrichment analysis showed that these DEGs were involved in “oxidoreductase activity”, “glycolysis/gluconeogenesis”, “MAPK signaling pathways”, and “ribosomes”. Our results support further understanding of the transcriptional changes and molecular processes underlying primordium formation and differentiation of <i>P. tuoliensis</i>.
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