Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor
Highly contagious COVID-19 disease is caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which poses a serious threat to global public health. Therefore, the development of a fast and reliable method for the detection of SARS-CoV-2 is an urgent research need. The Fe3O4@S...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Advanced Sensor and Energy Materials |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773045X23000225 |
| _version_ | 1797682794374103040 |
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| author | Xun-Hai You Yao Liu Yan-Yan Li Bing Zhao Yong Yang Rohan Weerasooriya Xing Chen |
| author_facet | Xun-Hai You Yao Liu Yan-Yan Li Bing Zhao Yong Yang Rohan Weerasooriya Xing Chen |
| author_sort | Xun-Hai You |
| collection | DOAJ |
| description | Highly contagious COVID-19 disease is caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which poses a serious threat to global public health. Therefore, the development of a fast and reliable method for the detection of SARS-CoV-2 is an urgent research need. The Fe3O4@SiO2–Au is enriched with a variety of functional groups, which can be used to fabricate a sensitive electrochemical biosensor by biofunctionalization with angiotensin-converting enzyme 2 (ACE2). Accordingly, we developed a novel electrochemical sensor by chemically modifying a glassy carbon electrode (GCE) with Fe3O4@SiO2–Au nanocomposites (hereafter Fe3O4@SiO2–Au/GCE) for the rapid detection of S-protein spiked SARS-CoV-2 by electrochemical impedance spectroscopy (EIS). The new electrochemical sensor has a low limit detection (viz., 4.78 pg/mL) and a wide linear dynamic range (viz., 0.1 ng/mL to 10 μg/mL) for detecting the EIS response signal of S-protein. The robust Fe3O4@SiO2–Au/GCE biosensor has high selectivity, stability, and reproducibility for the detection of S-protein with good recovery of saliva samples. |
| first_indexed | 2024-03-12T00:04:59Z |
| format | Article |
| id | doaj.art-41c545ca7e0d485d8987e8c09bf6f50c |
| institution | Directory Open Access Journal |
| issn | 2773-045X |
| language | English |
| last_indexed | 2024-03-12T00:04:59Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advanced Sensor and Energy Materials |
| spelling | doaj.art-41c545ca7e0d485d8987e8c09bf6f50c2023-09-17T04:58:05ZengElsevierAdvanced Sensor and Energy Materials2773-045X2023-09-0123100067Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensorXun-Hai You0Yao Liu1Yan-Yan Li2Bing Zhao3Yong Yang4Rohan Weerasooriya5Xing Chen6Key Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei 230009, PR China; School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, PR ChinaKey Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei 230009, PR China; School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, PR ChinaState Key Laboratory of High-Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR ChinaKey Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei 230009, PR China; School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, PR ChinaState Key Laboratory of High-Performance Ceramics and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, PR ChinaKey Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei 230009, PR China; National Centre for Water Quality Research, National Institute of Fundamental Studies, Kandy, 20 000, Sri LankaKey Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei 230009, PR China; School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, PR China; School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, PR China; National Centre for Water Quality Research, National Institute of Fundamental Studies, Kandy, 20 000, Sri Lanka; Corresponding author.Highly contagious COVID-19 disease is caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which poses a serious threat to global public health. Therefore, the development of a fast and reliable method for the detection of SARS-CoV-2 is an urgent research need. The Fe3O4@SiO2–Au is enriched with a variety of functional groups, which can be used to fabricate a sensitive electrochemical biosensor by biofunctionalization with angiotensin-converting enzyme 2 (ACE2). Accordingly, we developed a novel electrochemical sensor by chemically modifying a glassy carbon electrode (GCE) with Fe3O4@SiO2–Au nanocomposites (hereafter Fe3O4@SiO2–Au/GCE) for the rapid detection of S-protein spiked SARS-CoV-2 by electrochemical impedance spectroscopy (EIS). The new electrochemical sensor has a low limit detection (viz., 4.78 pg/mL) and a wide linear dynamic range (viz., 0.1 ng/mL to 10 μg/mL) for detecting the EIS response signal of S-protein. The robust Fe3O4@SiO2–Au/GCE biosensor has high selectivity, stability, and reproducibility for the detection of S-protein with good recovery of saliva samples.http://www.sciencedirect.com/science/article/pii/S2773045X23000225SARS-CoV-2 spike proteinFe3O4@SiO2–Au nanocompositesElectrochemical biosensorElectrochemical impedance spectroscopy |
| spellingShingle | Xun-Hai You Yao Liu Yan-Yan Li Bing Zhao Yong Yang Rohan Weerasooriya Xing Chen Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor Advanced Sensor and Energy Materials SARS-CoV-2 spike protein Fe3O4@SiO2–Au nanocomposites Electrochemical biosensor Electrochemical impedance spectroscopy |
| title | Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor |
| title_full | Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor |
| title_fullStr | Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor |
| title_full_unstemmed | Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor |
| title_short | Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor |
| title_sort | sensitive detection of sars cov 2 spike protein based on electrochemical impedance spectroscopy of fe3o4 sio2 au gce biosensor |
| topic | SARS-CoV-2 spike protein Fe3O4@SiO2–Au nanocomposites Electrochemical biosensor Electrochemical impedance spectroscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2773045X23000225 |
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