Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry
The molybdenum (Mo)-catalyzed oxidation of sulfide under neutral conditions yields sulfone. This reaction proceeds more smoothly than olefin epoxidation and primary or secondary alcohol oxidation. In this study, Mo-catalyzed oxidation was used to screen for sulfur compounds (named “MoS-scr...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/2/240 |
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| author | Hirotaka Matsuo Yu Hanamure Rei Miyano Yōko Takahashi Satoshi Ōmura Takuji Nakashima |
| author_facet | Hirotaka Matsuo Yu Hanamure Rei Miyano Yōko Takahashi Satoshi Ōmura Takuji Nakashima |
| author_sort | Hirotaka Matsuo |
| collection | DOAJ |
| description | The molybdenum (Mo)-catalyzed oxidation of sulfide under neutral conditions yields sulfone. This reaction proceeds more smoothly than olefin epoxidation and primary or secondary alcohol oxidation. In this study, Mo-catalyzed oxidation was used to screen for sulfur compounds (named “MoS-screening”) in microbial broths by liquid chromatography-mass spectrometry (LC/MS). To demonstrate proof-of-concept, known sulfur microbial compounds were successfully identified from a mixture of non-sulfur microbial compounds as sulfinyl or sulfonyl products of Mo-catalyzed oxidation. Then our MoS-screening method was used to screen 300 samples of microbial broth for sulfur compounds. One of the identified compounds was a kitasetaline-containing <i>N</i>-acetyl cysteine moiety produced by an actinomycete strain. These results demonstrate the potential of MoS-screening in the search for new sulfur compounds from microbial sources. |
| first_indexed | 2024-12-11T18:42:23Z |
| format | Article |
| id | doaj.art-41ca103814ec4fc39c37a1d52fbb018e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T18:42:23Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-41ca103814ec4fc39c37a1d52fbb018e2022-12-22T00:54:34ZengMDPI AGMolecules1420-30492020-01-0125224010.3390/molecules25020240molecules25020240Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass SpectrometryHirotaka Matsuo0Yu Hanamure1Rei Miyano2Yōko Takahashi3Satoshi Ōmura4Takuji Nakashima5Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanKitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanGraduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanKitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanKitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanKitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, JapanThe molybdenum (Mo)-catalyzed oxidation of sulfide under neutral conditions yields sulfone. This reaction proceeds more smoothly than olefin epoxidation and primary or secondary alcohol oxidation. In this study, Mo-catalyzed oxidation was used to screen for sulfur compounds (named “MoS-screening”) in microbial broths by liquid chromatography-mass spectrometry (LC/MS). To demonstrate proof-of-concept, known sulfur microbial compounds were successfully identified from a mixture of non-sulfur microbial compounds as sulfinyl or sulfonyl products of Mo-catalyzed oxidation. Then our MoS-screening method was used to screen 300 samples of microbial broth for sulfur compounds. One of the identified compounds was a kitasetaline-containing <i>N</i>-acetyl cysteine moiety produced by an actinomycete strain. These results demonstrate the potential of MoS-screening in the search for new sulfur compounds from microbial sources.https://www.mdpi.com/1420-3049/25/2/240microbial metabolitesmolybdenum-catalyzed oxidationmos-screeningscreening methodsulfoxidationsulfur compounds |
| spellingShingle | Hirotaka Matsuo Yu Hanamure Rei Miyano Yōko Takahashi Satoshi Ōmura Takuji Nakashima Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry Molecules microbial metabolites molybdenum-catalyzed oxidation mos-screening screening method sulfoxidation sulfur compounds |
| title | Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry |
| title_full | Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry |
| title_fullStr | Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry |
| title_full_unstemmed | Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry |
| title_short | Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry |
| title_sort | screening for sulfur compounds by molybdenum catalyzed oxidation combined with liquid chromatography mass spectrometry |
| topic | microbial metabolites molybdenum-catalyzed oxidation mos-screening screening method sulfoxidation sulfur compounds |
| url | https://www.mdpi.com/1420-3049/25/2/240 |
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