Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase

Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH)...

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Bibliographic Details
Main Authors: Edward King, Sarah Maxel, Yulai Zhang, Karissa C. Kenney, Youtian Cui, Emma Luu, Justin B. Siegel, Gregory A. Weiss, Ray Luo, Han Li
Format: Article
Language:English
Published: Nature Portfolio 2022-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-35021-x
Description
Summary:Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH).
ISSN:2041-1723

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