Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase
Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH)...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-35021-x |
| _version_ | 1811186871871471616 |
|---|---|
| author | Edward King Sarah Maxel Yulai Zhang Karissa C. Kenney Youtian Cui Emma Luu Justin B. Siegel Gregory A. Weiss Ray Luo Han Li |
| author_facet | Edward King Sarah Maxel Yulai Zhang Karissa C. Kenney Youtian Cui Emma Luu Justin B. Siegel Gregory A. Weiss Ray Luo Han Li |
| author_sort | Edward King |
| collection | DOAJ |
| description | Engineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH). |
| first_indexed | 2024-04-11T13:53:33Z |
| format | Article |
| id | doaj.art-41cd426163c543aaa20d2d073663935b |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T13:53:33Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-41cd426163c543aaa20d2d073663935b2022-12-22T04:20:27ZengNature PortfolioNature Communications2041-17232022-11-0113111410.1038/s41467-022-35021-xOrthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidaseEdward King0Sarah Maxel1Yulai Zhang2Karissa C. Kenney3Youtian Cui4Emma Luu5Justin B. Siegel6Gregory A. Weiss7Ray Luo8Han Li9Department of Molecular Biology and Biochemistry, University of California IrvineDepartment Chemical and Biomolecular Engineering University of California IrvineDepartment Chemical and Biomolecular Engineering University of California IrvineDepartment of Chemistry, University of California IrvineGenome Center, University of California DavisGenome Center, University of California DavisGenome Center, University of California DavisDepartment of Molecular Biology and Biochemistry, University of California IrvineDepartment of Molecular Biology and Biochemistry, University of California IrvineDepartment Chemical and Biomolecular Engineering University of California IrvineEngineering enzymes to accept noncanonical cofactor biomimetics is difficult. Here, the authors establish a self-sufficient growth selection method and demonstrate its application in engineering the Lactobacillus pentosus NADH oxidase to efficiently recycle reduced nicotinamide mononucleotide (NMNH).https://doi.org/10.1038/s41467-022-35021-x |
| spellingShingle | Edward King Sarah Maxel Yulai Zhang Karissa C. Kenney Youtian Cui Emma Luu Justin B. Siegel Gregory A. Weiss Ray Luo Han Li Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase Nature Communications |
| title | Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase |
| title_full | Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase |
| title_fullStr | Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase |
| title_full_unstemmed | Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase |
| title_short | Orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase |
| title_sort | orthogonal glycolytic pathway enables directed evolution of noncanonical cofactor oxidase |
| url | https://doi.org/10.1038/s41467-022-35021-x |
| work_keys_str_mv | AT edwardking orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT sarahmaxel orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT yulaizhang orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT karissackenney orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT youtiancui orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT emmaluu orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT justinbsiegel orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT gregoryaweiss orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT rayluo orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase AT hanli orthogonalglycolyticpathwayenablesdirectedevolutionofnoncanonicalcofactoroxidase |