Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population
Background: Type 2 diabetes mellitus (T2DM) is an inflammatory autoimmune disease that mostly affects older adults. The etiology of T2DM includes both genetic and environmental factors. rs1800795 (−174 G/C) single nucleotide polymorphism (SNP) linked with autoimmune disorders predispositions, identi...
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| Format: | Article |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2016;volume=5;issue=1;spage=18;epage=18;aulast=Ghavimi |
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| author | Reza Ghavimi Mohammadreza Sharifi Mohammad Ali Mohaghegh Hossein Mohammadian Saedeh Khadempar Hamzeh Rezaei |
| author_facet | Reza Ghavimi Mohammadreza Sharifi Mohammad Ali Mohaghegh Hossein Mohammadian Saedeh Khadempar Hamzeh Rezaei |
| author_sort | Reza Ghavimi |
| collection | DOAJ |
| description | Background: Type 2 diabetes mellitus (T2DM) is an inflammatory autoimmune disease that mostly affects older adults. The etiology of T2DM includes both genetic and environmental factors. rs1800795 (−174 G/C) single nucleotide polymorphism (SNP) linked with autoimmune disorders predispositions, identified by Genome-Wide Association Study among genes, which immunologically related is considerably over signified. The goal of this study was to evaluate the association between rs1800795 (−174 G/C) polymorphisms in the promoter of interleukin-6 (IL-6) gene with susceptibility to T2DM in a subset of the Iranian population.
Materials and Methods: In this case–control study, 120 healthy subjects and 120 patients with T2DM were included. Genomic DNA obtained from whole blood samples and the polymerase chain reaction was used to amplify the fragment of interest contain rs1800795 SNP, restriction fragment length polymorphism method was applied for genotyping of the DNA samples with NlaIII as a restriction enzyme. SPSS for Windows software (version 18.0, SPSS, Chicago, IL, USA) was performed for statistical analysis.
Results: No significant differences were found between healthy controls and T2DM patients with respect to the frequency distribution of the cytokine gene polymorphism investigated. Odds ratio, adjusted for sex, age, and smoking status has displayed similar outcomes.
Conclusion: These results indicated that the rs1800795 SNP is not a susceptibility gene variant for the development of T2DM in the Isfahan population. Further studies using new data on complex transcriptional interactions between IL-6 polymorphic sites are necessary to determine IL-6 haplotype influence on susceptibility to T2DM. |
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| format | Article |
| id | doaj.art-41cdb82a1baf4e6b9075fede94c41d0e |
| institution | Directory Open Access Journal |
| issn | 2277-9175 |
| language | English |
| last_indexed | 2024-12-21T20:35:40Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-41cdb82a1baf4e6b9075fede94c41d0e2022-12-21T18:51:06ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752016-01-0151181810.4103/2277-9175.175904Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan populationReza GhavimiMohammadreza SharifiMohammad Ali MohagheghHossein MohammadianSaedeh KhademparHamzeh RezaeiBackground: Type 2 diabetes mellitus (T2DM) is an inflammatory autoimmune disease that mostly affects older adults. The etiology of T2DM includes both genetic and environmental factors. rs1800795 (−174 G/C) single nucleotide polymorphism (SNP) linked with autoimmune disorders predispositions, identified by Genome-Wide Association Study among genes, which immunologically related is considerably over signified. The goal of this study was to evaluate the association between rs1800795 (−174 G/C) polymorphisms in the promoter of interleukin-6 (IL-6) gene with susceptibility to T2DM in a subset of the Iranian population. Materials and Methods: In this case–control study, 120 healthy subjects and 120 patients with T2DM were included. Genomic DNA obtained from whole blood samples and the polymerase chain reaction was used to amplify the fragment of interest contain rs1800795 SNP, restriction fragment length polymorphism method was applied for genotyping of the DNA samples with NlaIII as a restriction enzyme. SPSS for Windows software (version 18.0, SPSS, Chicago, IL, USA) was performed for statistical analysis. Results: No significant differences were found between healthy controls and T2DM patients with respect to the frequency distribution of the cytokine gene polymorphism investigated. Odds ratio, adjusted for sex, age, and smoking status has displayed similar outcomes. Conclusion: These results indicated that the rs1800795 SNP is not a susceptibility gene variant for the development of T2DM in the Isfahan population. Further studies using new data on complex transcriptional interactions between IL-6 polymorphic sites are necessary to determine IL-6 haplotype influence on susceptibility to T2DM.http://www.advbiores.net/article.asp?issn=2277-9175;year=2016;volume=5;issue=1;spage=18;epage=18;aulast=GhavimiGenome-wide association studyinterleukin-6 genepolymorphismtype 2 diabetes mellitus |
| spellingShingle | Reza Ghavimi Mohammadreza Sharifi Mohammad Ali Mohaghegh Hossein Mohammadian Saedeh Khadempar Hamzeh Rezaei Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population Advanced Biomedical Research Genome-wide association study interleukin-6 gene polymorphism type 2 diabetes mellitus |
| title | Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population |
| title_full | Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population |
| title_fullStr | Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population |
| title_full_unstemmed | Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population |
| title_short | Lack of association between rs1800795 (-174 G/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetes in Isfahan population |
| title_sort | lack of association between rs1800795 174 g c polymorphism in the promoter region of interleukin 6 gene and susceptibility to type 2 diabetes in isfahan population |
| topic | Genome-wide association study interleukin-6 gene polymorphism type 2 diabetes mellitus |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2016;volume=5;issue=1;spage=18;epage=18;aulast=Ghavimi |
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