Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production
Moderate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which facilitates the oil scavenging activity in the oil polluted surroundings. Production of such unusual bioactive molecules plays a vital role for their survival in an extreme and adverse environment. Current...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00235/full |
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| author | Samadhan Waghmode Mangesh Suryavanshi Laxmikant Dama Shraddha Kansara Vikas Ghattargi Parijat Das Arun Banpurkar Surekha K. Satpute |
| author_facet | Samadhan Waghmode Mangesh Suryavanshi Laxmikant Dama Shraddha Kansara Vikas Ghattargi Parijat Das Arun Banpurkar Surekha K. Satpute |
| author_sort | Samadhan Waghmode |
| collection | DOAJ |
| description | Moderate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which facilitates the oil scavenging activity in the oil polluted surroundings. Production of such unusual bioactive molecules plays a vital role for their survival in an extreme and adverse environment. Current research deals with isolation of Planococcus maritimus strain SAMP MCC 3013 from Indian Arabian coastline sea water for BS production. The bacterium tolerated up to 2.7 M NaCl demonstrating osmotic stress bearable physiological systems. We used integrated approach to explore the genomic insight of the strain SAMP and displayed the presence of gene for BS biosynthesis. The genome analysis revealed this potential to be intrinsic to the strain. Preliminary screening techniques viz., surface tension (SFT), drop collapse (DC) and oil displacement (OD) showed SAMP MCC 3013 as a potent BS producer. BS reduced SFT of phosphate buffer saline (PBS) pH: 7.0 from 72 to 30 mN/m with a critical micelle concentration (CMC) value of 1.3 mg/mL. Subsequent investigation on chemical characterization, using thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (1H NMR and 13C NMR) and liquid chromatography mass spectrometry (LC-MS) revealed terpene containing BS having sugar, lipid moieties. The genomic sequence analysis of P. maritimus SAMP showed complete genes in the pathway for the synthesis of terpenoid. Probably terpenoid is the accountable backbone molecule for the BS production, but the later stages of terpenoid conversion to the BS could not be found. Moreover, it is important to highlight that till today; no single report documents the in-detailed physico-chemical characterization of BS from Planococcus sp. Based on genomic and functional properties, the term terpene containing BS is denoted for the surfactant produced by P. maritimus. |
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| format | Article |
| id | doaj.art-41d01ce678f4430bbf07f45cf54f44fc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T10:20:04Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-41d01ce678f4430bbf07f45cf54f44fc2022-12-22T03:37:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00235413524Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant ProductionSamadhan Waghmode0Mangesh Suryavanshi1Laxmikant Dama2Shraddha Kansara3Vikas Ghattargi4Parijat Das5Arun Banpurkar6Surekha K. Satpute7Department of Microbiology, Elphinstone College, University of Mumbai, Mumbai, IndiaNational Centre for Microbial Resource, National Centre for Cell Science, Pune, IndiaDepartment of Zoology, DBF Dayanand College, University of Solapur, Solapur, IndiaDepartment of Microbiology, Savitribai Phule Pune University, Pune, IndiaNational Centre for Microbial Resource, National Centre for Cell Science, Pune, IndiaDepartment of Microbiology, Savitribai Phule Pune University, Pune, IndiaDepartment of Physics, Savitribai Phule Pune University, Pune, IndiaDepartment of Microbiology, Savitribai Phule Pune University, Pune, IndiaModerate halophilic bacteria thrive in saline conditions and produce biosurfactant (BS) which facilitates the oil scavenging activity in the oil polluted surroundings. Production of such unusual bioactive molecules plays a vital role for their survival in an extreme and adverse environment. Current research deals with isolation of Planococcus maritimus strain SAMP MCC 3013 from Indian Arabian coastline sea water for BS production. The bacterium tolerated up to 2.7 M NaCl demonstrating osmotic stress bearable physiological systems. We used integrated approach to explore the genomic insight of the strain SAMP and displayed the presence of gene for BS biosynthesis. The genome analysis revealed this potential to be intrinsic to the strain. Preliminary screening techniques viz., surface tension (SFT), drop collapse (DC) and oil displacement (OD) showed SAMP MCC 3013 as a potent BS producer. BS reduced SFT of phosphate buffer saline (PBS) pH: 7.0 from 72 to 30 mN/m with a critical micelle concentration (CMC) value of 1.3 mg/mL. Subsequent investigation on chemical characterization, using thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (1H NMR and 13C NMR) and liquid chromatography mass spectrometry (LC-MS) revealed terpene containing BS having sugar, lipid moieties. The genomic sequence analysis of P. maritimus SAMP showed complete genes in the pathway for the synthesis of terpenoid. Probably terpenoid is the accountable backbone molecule for the BS production, but the later stages of terpenoid conversion to the BS could not be found. Moreover, it is important to highlight that till today; no single report documents the in-detailed physico-chemical characterization of BS from Planococcus sp. Based on genomic and functional properties, the term terpene containing BS is denoted for the surfactant produced by P. maritimus.https://www.frontiersin.org/article/10.3389/fmicb.2019.00235/fullbiosurfactanthalophilePlanococcusterpenegenome sequencingmetabolite |
| spellingShingle | Samadhan Waghmode Mangesh Suryavanshi Laxmikant Dama Shraddha Kansara Vikas Ghattargi Parijat Das Arun Banpurkar Surekha K. Satpute Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production Frontiers in Microbiology biosurfactant halophile Planococcus terpene genome sequencing metabolite |
| title | Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production |
| title_full | Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production |
| title_fullStr | Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production |
| title_full_unstemmed | Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production |
| title_short | Genomic Insights of Halophilic Planococcus maritimus SAMP MCC 3013 and Detail Investigation of Its Biosurfactant Production |
| title_sort | genomic insights of halophilic planococcus maritimus samp mcc 3013 and detail investigation of its biosurfactant production |
| topic | biosurfactant halophile Planococcus terpene genome sequencing metabolite |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00235/full |
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