Polymorphism of the IL-10 gene in Azeri population of Iran
Abstract Background Interleukin (IL)-10 is one of the key interleukins in the immune system. It plays an anti-inflammatory role in body by inhibition of the synthesis of pro-inflammatory cytokines and reducing the expression of major histocompatibility complex II molecules. The single-nucleotide pol...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-07-01
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| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43042-022-00324-7 |
| _version_ | 1818192950945906688 |
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| author | Mohammad Asgharzadeh Zahra Taghinejad Vahid Asgharzadeh Bahareh Mehramouz Jalil Rashedi Behroz Mahdavipoor Mahya Pourostadi Ali Vegari Ali Safarzad Vishkaei Sepehr Taghizadeh Hossein Samadi Kafil |
| author_facet | Mohammad Asgharzadeh Zahra Taghinejad Vahid Asgharzadeh Bahareh Mehramouz Jalil Rashedi Behroz Mahdavipoor Mahya Pourostadi Ali Vegari Ali Safarzad Vishkaei Sepehr Taghizadeh Hossein Samadi Kafil |
| author_sort | Mohammad Asgharzadeh |
| collection | DOAJ |
| description | Abstract Background Interleukin (IL)-10 is one of the key interleukins in the immune system. It plays an anti-inflammatory role in body by inhibition of the synthesis of pro-inflammatory cytokines and reducing the expression of major histocompatibility complex II molecules. The single-nucleotide polymorphism in the gene of this interleukin affects its expression level. Thus, this study was conducted to investigate the IL-10 gene polymorphism at position -1082A/G in Azeri population of Iran. Methods Blood samples were taken from 254 healthy and non-relevant Iranian Azeri individuals. After DNA extraction, the frequency of IL-10 genotypes and alleles at -1082A/G position was determined by allele specific-PCR method. Then, q-square test was used to compare allele frequencies and IL-10 genotypes with other populations, and p value of < 0.05 was considered significant. Results In Iranian Azeri population, the frequency percentage of AA, AG and GG genotypes in IL-10 gene at the -1082A/G location was 37.4, 43.3 and 19.3%, respectively. The frequency percentage of A and G alleles also were 59.1 and 40.9%, respectively. Based on statistical analysis, frequency of IL-10 genotypes in the current study was very similar to the population of Saudi Arabia, but it had a significant difference with East Asia and Ireland populations. Conclusion Results of the present study indicate similar polymorphism of IL-10 genotype with neighbor ethnicities in Middle East country. Based on patients backgrounds mentioned in their questioners, this polymorphism was associated with the susceptibility to asthma and Alzheimer in this population which are common in the region. |
| first_indexed | 2024-12-12T00:38:39Z |
| format | Article |
| id | doaj.art-41d4cd20be654fb78ebfe3671144b160 |
| institution | Directory Open Access Journal |
| issn | 2090-2441 |
| language | English |
| last_indexed | 2024-12-12T00:38:39Z |
| publishDate | 2022-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-41d4cd20be654fb78ebfe3671144b1602022-12-22T00:44:18ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412022-07-012311610.1186/s43042-022-00324-7Polymorphism of the IL-10 gene in Azeri population of IranMohammad Asgharzadeh0Zahra Taghinejad1Vahid Asgharzadeh2Bahareh Mehramouz3Jalil Rashedi4Behroz Mahdavipoor5Mahya Pourostadi6Ali Vegari7Ali Safarzad Vishkaei8Sepehr Taghizadeh9Hossein Samadi Kafil10Biotechnology Research Center, Faculty of Paramedicine, Tabriz University of Medical SciencesHematology and Oncology Research Center, Tabriz University of Medical SciencesStudent Research Committee, Tabriz University of Medical SciencesBiotechnology Research Center, Faculty of Paramedicine, Tabriz University of Medical SciencesTuberculosis and Lung Disease Research Center, Tabriz University of Medical SciencesDepartment of Laboratory Sciences, Faculty of Paramedicine, Tabriz University of Medical SciencesMedical Philosophy and History Research Center, Tabriz University of Medical SciencesDepartment of Medical Physics, Faculty of Medicine, Urmia University of Medical SciencesKidney Research Center, Tabriz University of Medical SciencesHematology and Oncology Research Center, Tabriz University of Medical SciencesDrug Applied Research Center, Tabriz University of Medical SciencesAbstract Background Interleukin (IL)-10 is one of the key interleukins in the immune system. It plays an anti-inflammatory role in body by inhibition of the synthesis of pro-inflammatory cytokines and reducing the expression of major histocompatibility complex II molecules. The single-nucleotide polymorphism in the gene of this interleukin affects its expression level. Thus, this study was conducted to investigate the IL-10 gene polymorphism at position -1082A/G in Azeri population of Iran. Methods Blood samples were taken from 254 healthy and non-relevant Iranian Azeri individuals. After DNA extraction, the frequency of IL-10 genotypes and alleles at -1082A/G position was determined by allele specific-PCR method. Then, q-square test was used to compare allele frequencies and IL-10 genotypes with other populations, and p value of < 0.05 was considered significant. Results In Iranian Azeri population, the frequency percentage of AA, AG and GG genotypes in IL-10 gene at the -1082A/G location was 37.4, 43.3 and 19.3%, respectively. The frequency percentage of A and G alleles also were 59.1 and 40.9%, respectively. Based on statistical analysis, frequency of IL-10 genotypes in the current study was very similar to the population of Saudi Arabia, but it had a significant difference with East Asia and Ireland populations. Conclusion Results of the present study indicate similar polymorphism of IL-10 genotype with neighbor ethnicities in Middle East country. Based on patients backgrounds mentioned in their questioners, this polymorphism was associated with the susceptibility to asthma and Alzheimer in this population which are common in the region.https://doi.org/10.1186/s43042-022-00324-7IL-10GenotypeSingle-nucleotide polymorphismsIranian Azeri population |
| spellingShingle | Mohammad Asgharzadeh Zahra Taghinejad Vahid Asgharzadeh Bahareh Mehramouz Jalil Rashedi Behroz Mahdavipoor Mahya Pourostadi Ali Vegari Ali Safarzad Vishkaei Sepehr Taghizadeh Hossein Samadi Kafil Polymorphism of the IL-10 gene in Azeri population of Iran Egyptian Journal of Medical Human Genetics IL-10 Genotype Single-nucleotide polymorphisms Iranian Azeri population |
| title | Polymorphism of the IL-10 gene in Azeri population of Iran |
| title_full | Polymorphism of the IL-10 gene in Azeri population of Iran |
| title_fullStr | Polymorphism of the IL-10 gene in Azeri population of Iran |
| title_full_unstemmed | Polymorphism of the IL-10 gene in Azeri population of Iran |
| title_short | Polymorphism of the IL-10 gene in Azeri population of Iran |
| title_sort | polymorphism of the il 10 gene in azeri population of iran |
| topic | IL-10 Genotype Single-nucleotide polymorphisms Iranian Azeri population |
| url | https://doi.org/10.1186/s43042-022-00324-7 |
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