Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation
Abstract Background Sigesbeckia glabrescens Makino (SG) has been traditionally used for rheumatism and joint protection. However, the anti-arthritic effects and underling mechanisms of SG have not been demonstrated. In this study, we investigated the anti-arthritic effects and mechanisms of SG extra...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | Chinese Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s13020-020-00372-4 |
| _version_ | 1818883153862852608 |
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| author | Qiu Shuo Ma Ke-Gang Linghu Tian Zhang Guan Ding Zhao Wei Xiong Shi Hang Xiong Mingming Zhao Wei Xu Juan Yu Hua Yu |
| author_facet | Qiu Shuo Ma Ke-Gang Linghu Tian Zhang Guan Ding Zhao Wei Xiong Shi Hang Xiong Mingming Zhao Wei Xu Juan Yu Hua Yu |
| author_sort | Qiu Shuo Ma |
| collection | DOAJ |
| description | Abstract Background Sigesbeckia glabrescens Makino (SG) has been traditionally used for rheumatism and joint protection. However, the anti-arthritic effects and underling mechanisms of SG have not been demonstrated. In this study, we investigated the anti-arthritic effects and mechanisms of SG extract (SGE) on collagen-induced arthritic rats and interleukin (IL)-1β-stimulated human synovial SW982 cells. Methods Rats were induced to arthritis by collagen for 15 days and then received SGE treatment for 35 days. The body weight and arthritis severity score of the rats were monitored weekly. At the end of the experiment, the radiographic and histological changes of rats’ hind paw were obtained; the serum C-reactive protein was detected by enzyme-linked immunosorbent assay (ELISA); the expression levels of interleukin (IL)- 1β, IL6 and IL-10 in the joint muscles were determined by ELISA and immunohistochemical staining; and the level of regulatory T cells (Tregs) in the spleen was detected using flow cytometry. In addition, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scratch wound healing assay were used to evaluate the proliferation of SW982 synovial cells. ELISA, western blot and immunofluorescence staining were used to investigate the anti-inflammatory mechanisms of SGE on IL-1β-induced SW982 cells and joint muscles of CIA rats. Results SGE attenuated the collagen-induced hind paw swelling, cartilage damage and bone erosion. SGE inhibited the synovial hyperplasia to the articular cavity in the toe joint and ankle. Moreover, SGE decreased the production of C-reactive protein in serum and the expression of IL-6, IL-1β, cyclooxygenase-2 (COX-2) and phosphorylation of NF-κB p65 in the joint muscles. SGE also recovered the decreased Tregs. Results from the in vitro experiments showed that SGE not only inhibited the proliferation and migration of human synovial cell but also inhibited the IL-1β-induced expression of IL-6 and IL-8. Similarly, SGE inhibited the activation of NF-κB and the expression of COX-2. Conclusions SGE attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation. |
| first_indexed | 2024-12-19T15:29:08Z |
| format | Article |
| id | doaj.art-41d6114a873f4aaaa18886b799f60908 |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-19T15:29:08Z |
| publishDate | 2020-08-01 |
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| series | Chinese Medicine |
| spelling | doaj.art-41d6114a873f4aaaa18886b799f609082022-12-21T20:15:47ZengBMCChinese Medicine1749-85462020-08-0115111210.1186/s13020-020-00372-4Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammationQiu Shuo Ma0Ke-Gang Linghu1Tian Zhang2Guan Ding Zhao3Wei Xiong4Shi Hang Xiong5Mingming Zhao6Wei Xu7Juan Yu8Hua Yu9Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauCollege of Pharmacy, Fujian University of Traditional Chinese MedicineZhangzhou Pientzehuang Pharmaceutical LtdInstitute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of MacauAbstract Background Sigesbeckia glabrescens Makino (SG) has been traditionally used for rheumatism and joint protection. However, the anti-arthritic effects and underling mechanisms of SG have not been demonstrated. In this study, we investigated the anti-arthritic effects and mechanisms of SG extract (SGE) on collagen-induced arthritic rats and interleukin (IL)-1β-stimulated human synovial SW982 cells. Methods Rats were induced to arthritis by collagen for 15 days and then received SGE treatment for 35 days. The body weight and arthritis severity score of the rats were monitored weekly. At the end of the experiment, the radiographic and histological changes of rats’ hind paw were obtained; the serum C-reactive protein was detected by enzyme-linked immunosorbent assay (ELISA); the expression levels of interleukin (IL)- 1β, IL6 and IL-10 in the joint muscles were determined by ELISA and immunohistochemical staining; and the level of regulatory T cells (Tregs) in the spleen was detected using flow cytometry. In addition, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scratch wound healing assay were used to evaluate the proliferation of SW982 synovial cells. ELISA, western blot and immunofluorescence staining were used to investigate the anti-inflammatory mechanisms of SGE on IL-1β-induced SW982 cells and joint muscles of CIA rats. Results SGE attenuated the collagen-induced hind paw swelling, cartilage damage and bone erosion. SGE inhibited the synovial hyperplasia to the articular cavity in the toe joint and ankle. Moreover, SGE decreased the production of C-reactive protein in serum and the expression of IL-6, IL-1β, cyclooxygenase-2 (COX-2) and phosphorylation of NF-κB p65 in the joint muscles. SGE also recovered the decreased Tregs. Results from the in vitro experiments showed that SGE not only inhibited the proliferation and migration of human synovial cell but also inhibited the IL-1β-induced expression of IL-6 and IL-8. Similarly, SGE inhibited the activation of NF-κB and the expression of COX-2. Conclusions SGE attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation.http://link.springer.com/article/10.1186/s13020-020-00372-4Rheumnnatoid arthritisSynovial hyperplasiaInflammationSigesbeckia glabrescens MakinoSW982Nuclear transcription factor-kappa B |
| spellingShingle | Qiu Shuo Ma Ke-Gang Linghu Tian Zhang Guan Ding Zhao Wei Xiong Shi Hang Xiong Mingming Zhao Wei Xu Juan Yu Hua Yu Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation Chinese Medicine Rheumnnatoid arthritis Synovial hyperplasia Inflammation Sigesbeckia glabrescens Makino SW982 Nuclear transcription factor-kappa B |
| title | Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation |
| title_full | Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation |
| title_fullStr | Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation |
| title_full_unstemmed | Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation |
| title_short | Sigesbeckia glabrescens Makino extract attenuated the collagen-induced arthritis through inhibiting the synovial hyperplasia and inflammation |
| title_sort | sigesbeckia glabrescens makino extract attenuated the collagen induced arthritis through inhibiting the synovial hyperplasia and inflammation |
| topic | Rheumnnatoid arthritis Synovial hyperplasia Inflammation Sigesbeckia glabrescens Makino SW982 Nuclear transcription factor-kappa B |
| url | http://link.springer.com/article/10.1186/s13020-020-00372-4 |
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