TGF-β Signaling Regulates the Differentiation of Motile Cilia
The cilium is a small cellular organelle with motility- and/or sensory-related functions that plays a crucial role during developmental and homeostatic processes. Although many molecules or signal transduction pathways that control cilia assembly have been reported, the mechanisms of ciliary length...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-05-01
|
| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112471500412X |
| _version_ | 1828268835784884224 |
|---|---|
| author | Janos Tözser Ryan Earwood Akiko Kato Jacob Brown Koichi Tanaka Ruth Didier Timothy L. Megraw Martin Blum Yoichi Kato |
| author_facet | Janos Tözser Ryan Earwood Akiko Kato Jacob Brown Koichi Tanaka Ruth Didier Timothy L. Megraw Martin Blum Yoichi Kato |
| author_sort | Janos Tözser |
| collection | DOAJ |
| description | The cilium is a small cellular organelle with motility- and/or sensory-related functions that plays a crucial role during developmental and homeostatic processes. Although many molecules or signal transduction pathways that control cilia assembly have been reported, the mechanisms of ciliary length control have remained enigmatic. Here, we report that Smad2-dependent transforming growth factor β (TGF-β) signaling impacts the length of motile cilia at the Xenopus left-right (LR) organizer, the gastrocoel roof plate (GRP), as well as at the neural tube and the epidermis. Blocking TGF-β signaling resulted in the absence of the transition zone protein B9D1/MSKR-1 from cilia in multi-ciliated cells (MCCs) of the epidermis. Interestingly, this TGF-β activity is not mediated by Mcidas, Foxj1, and RFX2, the known major regulators of ciliogenesis. These data indicate that TGF-β signaling is crucial for the function of the transition zone, which in turn may affect the regulation of cilia length. |
| first_indexed | 2024-04-13T05:23:50Z |
| format | Article |
| id | doaj.art-41d74edfbd4045d8bb9ac34c0f0af899 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-13T05:23:50Z |
| publishDate | 2015-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-41d74edfbd4045d8bb9ac34c0f0af8992022-12-22T03:00:40ZengElsevierCell Reports2211-12472015-05-011171000100710.1016/j.celrep.2015.04.025TGF-β Signaling Regulates the Differentiation of Motile CiliaJanos Tözser0Ryan Earwood1Akiko Kato2Jacob Brown3Koichi Tanaka4Ruth Didier5Timothy L. Megraw6Martin Blum7Yoichi Kato8Institute of Zoology, University of Hohenheim, Garbenstrtraβe 30, 70593 Stuttgart, GermanyDepartment of Biomedical Sciences, Florida State University College of Medicine, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Biomedical Sciences, Florida State University College of Medicine, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Biomedical Sciences, Florida State University College of Medicine, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Biomedical Sciences, Florida State University College of Medicine, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Biomedical Sciences, Florida State University College of Medicine, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Biomedical Sciences, Florida State University College of Medicine, 1115 West Call Street, Tallahassee, FL 32306-4300, USAInstitute of Zoology, University of Hohenheim, Garbenstrtraβe 30, 70593 Stuttgart, GermanyDepartment of Biomedical Sciences, Florida State University College of Medicine, 1115 West Call Street, Tallahassee, FL 32306-4300, USAThe cilium is a small cellular organelle with motility- and/or sensory-related functions that plays a crucial role during developmental and homeostatic processes. Although many molecules or signal transduction pathways that control cilia assembly have been reported, the mechanisms of ciliary length control have remained enigmatic. Here, we report that Smad2-dependent transforming growth factor β (TGF-β) signaling impacts the length of motile cilia at the Xenopus left-right (LR) organizer, the gastrocoel roof plate (GRP), as well as at the neural tube and the epidermis. Blocking TGF-β signaling resulted in the absence of the transition zone protein B9D1/MSKR-1 from cilia in multi-ciliated cells (MCCs) of the epidermis. Interestingly, this TGF-β activity is not mediated by Mcidas, Foxj1, and RFX2, the known major regulators of ciliogenesis. These data indicate that TGF-β signaling is crucial for the function of the transition zone, which in turn may affect the regulation of cilia length.http://www.sciencedirect.com/science/article/pii/S221112471500412X |
| spellingShingle | Janos Tözser Ryan Earwood Akiko Kato Jacob Brown Koichi Tanaka Ruth Didier Timothy L. Megraw Martin Blum Yoichi Kato TGF-β Signaling Regulates the Differentiation of Motile Cilia Cell Reports |
| title | TGF-β Signaling Regulates the Differentiation of Motile Cilia |
| title_full | TGF-β Signaling Regulates the Differentiation of Motile Cilia |
| title_fullStr | TGF-β Signaling Regulates the Differentiation of Motile Cilia |
| title_full_unstemmed | TGF-β Signaling Regulates the Differentiation of Motile Cilia |
| title_short | TGF-β Signaling Regulates the Differentiation of Motile Cilia |
| title_sort | tgf β signaling regulates the differentiation of motile cilia |
| url | http://www.sciencedirect.com/science/article/pii/S221112471500412X |
| work_keys_str_mv | AT janostozser tgfbsignalingregulatesthedifferentiationofmotilecilia AT ryanearwood tgfbsignalingregulatesthedifferentiationofmotilecilia AT akikokato tgfbsignalingregulatesthedifferentiationofmotilecilia AT jacobbrown tgfbsignalingregulatesthedifferentiationofmotilecilia AT koichitanaka tgfbsignalingregulatesthedifferentiationofmotilecilia AT ruthdidier tgfbsignalingregulatesthedifferentiationofmotilecilia AT timothylmegraw tgfbsignalingregulatesthedifferentiationofmotilecilia AT martinblum tgfbsignalingregulatesthedifferentiationofmotilecilia AT yoichikato tgfbsignalingregulatesthedifferentiationofmotilecilia |