Comparison of human B cell activation by TLR7 and TLR9 agonists
<p>Abstract</p> <p>Background</p> <p>Human B cells and plasmacytoid dendritic cells (pDC) are the only cells known to express both TLR7 and TLR9. Plasmacytoid dendritic cells are the primary IFN-α producing cells in response to TLR7 and TLR9 agonists. The direct effects...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-07-01
|
| Series: | BMC Immunology |
| Online Access: | http://www.biomedcentral.com/1471-2172/9/39 |
| _version_ | 1818489803684970496 |
|---|---|
| author | Neys Lori Riter Christie L Vasilakos John P Hanten John A Lipson Kenneth E Alkan Sefik S Birmachu Woubalem |
| author_facet | Neys Lori Riter Christie L Vasilakos John P Hanten John A Lipson Kenneth E Alkan Sefik S Birmachu Woubalem |
| author_sort | Neys Lori |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Human B cells and plasmacytoid dendritic cells (pDC) are the only cells known to express both TLR7 and TLR9. Plasmacytoid dendritic cells are the primary IFN-α producing cells in response to TLR7 and TLR9 agonists. The direct effects of TLR7 stimulation on human B cells is less understood. The objective of this study was to compare the effects of TLR7 and TLR9 stimulation on human B cell function.</p> <p>Results</p> <p>Gene expression and protein production of cytokines, chemokines, various B cell activation markers, and immunoglobulins were evaluated. Purified human CD19<sup>+ </sup>B cells (99.9%, containing both naïve and memory populations) from peripheral blood were stimulated with a TLR7-selective agonist (852A), TLR7/8 agonist (3M-003), or TLR9 selective agonist CpG ODN (CpG2006). TLR7 and TLR9 agonists similarly modulated the expression of cytokine and chemokine genes (IL-6, MIP1 alpha, MIP1 beta, TNF alpha and LTA), co-stimulatory molecules (CD80, CD40 and CD58), Fc receptors (CD23, CD32), anti-apoptotic genes (BCL2L1), certain transcription factors (MYC, TCFL5), and genes critical for B cell proliferation and differentiation (CD72, IL21R). Both agonists also induced protein expression of the above cytokines and chemokines. Additionally, TLR7 and TLR9 agonists induced the production of IgM and IgG. A TLR8-selective agonist was comparatively ineffective at stimulating purified human B cells.</p> <p>Conclusion</p> <p>These results demonstrate that despite their molecular differences, the TLR7 and TLR9 agonists induce similar genes and proteins in purified human B cells.</p> |
| first_indexed | 2024-12-10T17:08:20Z |
| format | Article |
| id | doaj.art-41d7a15293ec49c7b382a653ad5951c7 |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-10T17:08:20Z |
| publishDate | 2008-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-41d7a15293ec49c7b382a653ad5951c72022-12-22T01:40:24ZengBMCBMC Immunology1471-21722008-07-01913910.1186/1471-2172-9-39Comparison of human B cell activation by TLR7 and TLR9 agonistsNeys LoriRiter Christie LVasilakos John PHanten John ALipson Kenneth EAlkan Sefik SBirmachu Woubalem<p>Abstract</p> <p>Background</p> <p>Human B cells and plasmacytoid dendritic cells (pDC) are the only cells known to express both TLR7 and TLR9. Plasmacytoid dendritic cells are the primary IFN-α producing cells in response to TLR7 and TLR9 agonists. The direct effects of TLR7 stimulation on human B cells is less understood. The objective of this study was to compare the effects of TLR7 and TLR9 stimulation on human B cell function.</p> <p>Results</p> <p>Gene expression and protein production of cytokines, chemokines, various B cell activation markers, and immunoglobulins were evaluated. Purified human CD19<sup>+ </sup>B cells (99.9%, containing both naïve and memory populations) from peripheral blood were stimulated with a TLR7-selective agonist (852A), TLR7/8 agonist (3M-003), or TLR9 selective agonist CpG ODN (CpG2006). TLR7 and TLR9 agonists similarly modulated the expression of cytokine and chemokine genes (IL-6, MIP1 alpha, MIP1 beta, TNF alpha and LTA), co-stimulatory molecules (CD80, CD40 and CD58), Fc receptors (CD23, CD32), anti-apoptotic genes (BCL2L1), certain transcription factors (MYC, TCFL5), and genes critical for B cell proliferation and differentiation (CD72, IL21R). Both agonists also induced protein expression of the above cytokines and chemokines. Additionally, TLR7 and TLR9 agonists induced the production of IgM and IgG. A TLR8-selective agonist was comparatively ineffective at stimulating purified human B cells.</p> <p>Conclusion</p> <p>These results demonstrate that despite their molecular differences, the TLR7 and TLR9 agonists induce similar genes and proteins in purified human B cells.</p>http://www.biomedcentral.com/1471-2172/9/39 |
| spellingShingle | Neys Lori Riter Christie L Vasilakos John P Hanten John A Lipson Kenneth E Alkan Sefik S Birmachu Woubalem Comparison of human B cell activation by TLR7 and TLR9 agonists BMC Immunology |
| title | Comparison of human B cell activation by TLR7 and TLR9 agonists |
| title_full | Comparison of human B cell activation by TLR7 and TLR9 agonists |
| title_fullStr | Comparison of human B cell activation by TLR7 and TLR9 agonists |
| title_full_unstemmed | Comparison of human B cell activation by TLR7 and TLR9 agonists |
| title_short | Comparison of human B cell activation by TLR7 and TLR9 agonists |
| title_sort | comparison of human b cell activation by tlr7 and tlr9 agonists |
| url | http://www.biomedcentral.com/1471-2172/9/39 |
| work_keys_str_mv | AT neyslori comparisonofhumanbcellactivationbytlr7andtlr9agonists AT riterchristiel comparisonofhumanbcellactivationbytlr7andtlr9agonists AT vasilakosjohnp comparisonofhumanbcellactivationbytlr7andtlr9agonists AT hantenjohna comparisonofhumanbcellactivationbytlr7andtlr9agonists AT lipsonkennethe comparisonofhumanbcellactivationbytlr7andtlr9agonists AT alkansefiks comparisonofhumanbcellactivationbytlr7andtlr9agonists AT birmachuwoubalem comparisonofhumanbcellactivationbytlr7andtlr9agonists |