First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal Origin
We describe here the complete genome sequence of an <i>Enterobacter hormaechei</i> ST279 coharbouring <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i> recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/9/687 |
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| author | Mustafa Sadek Hirofumi Nariya Toshi Shimamoto Shizuo Kayama Liansheng Yu Junzo Hisatsune Motoyuki Sugai Patrice Nordmann Laurent Poirel Tadashi Shimamoto |
| author_facet | Mustafa Sadek Hirofumi Nariya Toshi Shimamoto Shizuo Kayama Liansheng Yu Junzo Hisatsune Motoyuki Sugai Patrice Nordmann Laurent Poirel Tadashi Shimamoto |
| author_sort | Mustafa Sadek |
| collection | DOAJ |
| description | We describe here the complete genome sequence of an <i>Enterobacter hormaechei</i> ST279 coharbouring <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i> recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but susceptible to colistin (minimum inhibitory concentration (MIC), 0.5 μg/mL). The antimicrobial susceptibility profile and conjugation experiments were performed. The entire genome was sequenced by the Illumina MiniSeq and Oxford Nanopore methods. The <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i> genes are carried on the same IncHI2/pMLST1 plasmid, pMS37a (Size of 270.9 kb). The <i>mcr-9</i> gene was located within the physical boundaries demarcated by two insertion elements IS<i>903</i> (upstream) and IS<i>1</i> (downstream) but did not possess the downstream regulatory genes (<i>qseC</i>/<i>qseB</i>) which regulate the expression of <i>mcr-9</i>. Therefore, the <i>mcr-9</i> might be silently disseminated among carbapenem-resistant Enterobacterales. In addition to <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i>, plasmid pMS37a harbored various antibiotic resistance genes including <i>aac(6’)-Il</i>, Δ<i>aadA22</i>, <i>aac(6’)-Ib-cr</i>, <i>sul1</i>, <i>dfrA1 and tetA.</i> To the best of our knowledge, this is the first report of a <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i>-coharbouring <i>E. hormaechei</i> isolate of food origin worldwide. The identification of a multidrug-resistant VIM-1 and <i>mcr-9</i> positive <i>Enterobacter hormaechei</i> isolate from food is worrisome as retail meat and meat products could serve as a vehicle for these MDR bacteria, which could be transferred between animals and humans through the food chain. It further highlights that Enterobacterales co-producing MCR and carbapenemases being found in the food chain indeed correspond to a One-Health issue, highlighting the need for serious steps to prevent their further dissemination. |
| first_indexed | 2024-03-10T16:59:44Z |
| format | Article |
| id | doaj.art-41d89e2d7250482ca35a381cc4dd5a32 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T16:59:44Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-41d89e2d7250482ca35a381cc4dd5a322023-11-20T10:59:49ZengMDPI AGPathogens2076-08172020-08-019968710.3390/pathogens9090687First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal OriginMustafa Sadek0Hirofumi Nariya1Toshi Shimamoto2Shizuo Kayama3Liansheng Yu4Junzo Hisatsune5Motoyuki Sugai6Patrice Nordmann7Laurent Poirel8Tadashi Shimamoto9Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandLaboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, JapanLaboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, JapanAntimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama 189-0002, JapanMedical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandMedical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandLaboratory of Food Microbiology and Hygiene, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, JapanWe describe here the complete genome sequence of an <i>Enterobacter hormaechei</i> ST279 coharbouring <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i> recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but susceptible to colistin (minimum inhibitory concentration (MIC), 0.5 μg/mL). The antimicrobial susceptibility profile and conjugation experiments were performed. The entire genome was sequenced by the Illumina MiniSeq and Oxford Nanopore methods. The <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i> genes are carried on the same IncHI2/pMLST1 plasmid, pMS37a (Size of 270.9 kb). The <i>mcr-9</i> gene was located within the physical boundaries demarcated by two insertion elements IS<i>903</i> (upstream) and IS<i>1</i> (downstream) but did not possess the downstream regulatory genes (<i>qseC</i>/<i>qseB</i>) which regulate the expression of <i>mcr-9</i>. Therefore, the <i>mcr-9</i> might be silently disseminated among carbapenem-resistant Enterobacterales. In addition to <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i>, plasmid pMS37a harbored various antibiotic resistance genes including <i>aac(6’)-Il</i>, Δ<i>aadA22</i>, <i>aac(6’)-Ib-cr</i>, <i>sul1</i>, <i>dfrA1 and tetA.</i> To the best of our knowledge, this is the first report of a <i>bla</i><sub>VIM-1</sub> and <i>mcr-9</i>-coharbouring <i>E. hormaechei</i> isolate of food origin worldwide. The identification of a multidrug-resistant VIM-1 and <i>mcr-9</i> positive <i>Enterobacter hormaechei</i> isolate from food is worrisome as retail meat and meat products could serve as a vehicle for these MDR bacteria, which could be transferred between animals and humans through the food chain. It further highlights that Enterobacterales co-producing MCR and carbapenemases being found in the food chain indeed correspond to a One-Health issue, highlighting the need for serious steps to prevent their further dissemination.https://www.mdpi.com/2076-0817/9/9/687<i>Enterobacter cloacae</i> complex<i>mcr-9</i>VIM-1IncHI2WGSEgypt |
| spellingShingle | Mustafa Sadek Hirofumi Nariya Toshi Shimamoto Shizuo Kayama Liansheng Yu Junzo Hisatsune Motoyuki Sugai Patrice Nordmann Laurent Poirel Tadashi Shimamoto First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal Origin Pathogens <i>Enterobacter cloacae</i> complex <i>mcr-9</i> VIM-1 IncHI2 WGS Egypt |
| title | First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal Origin |
| title_full | First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal Origin |
| title_fullStr | First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal Origin |
| title_full_unstemmed | First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal Origin |
| title_short | First Genomic Characterization of <i>bla</i><sub>VIM-1</sub> and <i>mcr-9-</i>Coharbouring <i>Enterobacter</i> <i>hormaechei</i> Isolated from Food of Animal Origin |
| title_sort | first genomic characterization of i bla i sub vim 1 sub and i mcr 9 i coharbouring i enterobacter i i hormaechei i isolated from food of animal origin |
| topic | <i>Enterobacter cloacae</i> complex <i>mcr-9</i> VIM-1 IncHI2 WGS Egypt |
| url | https://www.mdpi.com/2076-0817/9/9/687 |
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