Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect
Lowering blood cholesterol levels is crucial for reducing the risk of cardiovascular disease in patients with familial hypercholesterolemia. To develop <i>Perilla frutescens</i> (L.) Britt. leaves as a functional food with a cholesterol-lowering effect, in this study, we collected <i&...
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MDPI AG
2023-10-01
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| author | Zhaoyang Wu Sangyoun Lee Beomgoo Kang Sookyeong Lee Kyochul Koo Jaeyong Lee Soonsung Lim |
| author_facet | Zhaoyang Wu Sangyoun Lee Beomgoo Kang Sookyeong Lee Kyochul Koo Jaeyong Lee Soonsung Lim |
| author_sort | Zhaoyang Wu |
| collection | DOAJ |
| description | Lowering blood cholesterol levels is crucial for reducing the risk of cardiovascular disease in patients with familial hypercholesterolemia. To develop <i>Perilla frutescens</i> (L.) Britt. leaves as a functional food with a cholesterol-lowering effect, in this study, we collected <i>P. frutescens</i> (L.) Britt. leaves from different regions of China and Republic of Korea. On the basis of the extraction yield (all components; g/kg), we selected <i>P. frutescens</i> (L.) Britt. leaves from Hebei Province, China with an extract yield of 60.9 g/kg. After evaluating different concentrations of ethanol/water solvent for <i>P. frutescens</i> (L.) Britt. leaves, with luteolin 7-glucuronide as the indicator component, we selected a 30% ethanol/water solvent with a high luteolin 7-glucuronide content of 0.548 mg/g in <i>Perilla. frutescens</i> (L.) Britt. leaves. Subsequently, we evaluated the cholesterol-lowering effects of <i>P. frutescens</i> (L.) Britt. leaf extract and luteolin 7-glucuronide by detecting total cholesterol in HepG2 cells. The 30% ethanol extract lowered cholesterol levels significantly by downregulating 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase expression. This suggests that <i>P. frutescens</i> (L.) Britt leaves have significant health benefits and can be explored as a potentially promising food additive for the prevention of hypercholesterolemia-related diseases. |
| first_indexed | 2024-03-10T21:01:27Z |
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| language | English |
| last_indexed | 2024-03-10T21:01:27Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-41da6e55c041499791cef05bea04f9b82023-11-19T17:31:21ZengMDPI AGMolecules1420-30492023-10-012820700710.3390/molecules28207007Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering EffectZhaoyang Wu0Sangyoun Lee1Beomgoo Kang2Sookyeong Lee3Kyochul Koo4Jaeyong Lee5Soonsung Lim6Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of KoreaInstitute for Liver and Digestive Diseases, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of KoreaDepartment of Biochemistry, College of Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of KoreaCOSFarm Co., Ltd., Corporate Research Institute, 3F 162, Saeteo-gil, Seonggeo-eup, Seobuk-gu, Cheonan-si 12446, Republic of KoreaDepartment of Biochemistry, College of Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of KoreaDepartment of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon 24252, Republic of KoreaLowering blood cholesterol levels is crucial for reducing the risk of cardiovascular disease in patients with familial hypercholesterolemia. To develop <i>Perilla frutescens</i> (L.) Britt. leaves as a functional food with a cholesterol-lowering effect, in this study, we collected <i>P. frutescens</i> (L.) Britt. leaves from different regions of China and Republic of Korea. On the basis of the extraction yield (all components; g/kg), we selected <i>P. frutescens</i> (L.) Britt. leaves from Hebei Province, China with an extract yield of 60.9 g/kg. After evaluating different concentrations of ethanol/water solvent for <i>P. frutescens</i> (L.) Britt. leaves, with luteolin 7-glucuronide as the indicator component, we selected a 30% ethanol/water solvent with a high luteolin 7-glucuronide content of 0.548 mg/g in <i>Perilla. frutescens</i> (L.) Britt. leaves. Subsequently, we evaluated the cholesterol-lowering effects of <i>P. frutescens</i> (L.) Britt. leaf extract and luteolin 7-glucuronide by detecting total cholesterol in HepG2 cells. The 30% ethanol extract lowered cholesterol levels significantly by downregulating 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase expression. This suggests that <i>P. frutescens</i> (L.) Britt leaves have significant health benefits and can be explored as a potentially promising food additive for the prevention of hypercholesterolemia-related diseases.https://www.mdpi.com/1420-3049/28/20/7007<i>Perilla frutescens</i> (L.) Britt.luteolin-7-diglucuronidehypercholesterolemia cholesterol-lowering effect |
| spellingShingle | Zhaoyang Wu Sangyoun Lee Beomgoo Kang Sookyeong Lee Kyochul Koo Jaeyong Lee Soonsung Lim Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect Molecules <i>Perilla frutescens</i> (L.) Britt. luteolin-7-diglucuronide hypercholesterolemia cholesterol-lowering effect |
| title | Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect |
| title_full | Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect |
| title_fullStr | Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect |
| title_full_unstemmed | Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect |
| title_short | Determination of Luteolin 7-Glucuronide in <i>Perilla frutescens</i> (L.) Britt. Leaf Extracts from Different Regions of China and Republic of Korea and Its Cholesterol-Lowering Effect |
| title_sort | determination of luteolin 7 glucuronide in i perilla frutescens i l britt leaf extracts from different regions of china and republic of korea and its cholesterol lowering effect |
| topic | <i>Perilla frutescens</i> (L.) Britt. luteolin-7-diglucuronide hypercholesterolemia cholesterol-lowering effect |
| url | https://www.mdpi.com/1420-3049/28/20/7007 |
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