The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species
Background Two endemic lycophyte species Isoetes cangae and Isoetes serracarajensis have been recently described in the State of Pará in the Amazon forest located in northern Brazil. Isoetes L. has survived through three mass extinctions. Plants are considered small-sized, heterosporous, and can dis...
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PeerJ Inc.
2020-11-01
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| author | Mirella Pupo Santos João V.S. Rabelo Araujo Arthur V. Sant’anna Lopes Julio Cesar Fiorio Vettorazzi Marcela Santana Bastos Boechat Fernanda AbreuSantana Arêdes Naiara Viana Campos Emiliano Nicolas Calderon Fernando M. Gomes Santos Tais Nogueira Fernandes Rodrigo Nunes da Fonseca Messias Gonzaga Pereira Guilherme Oliveira Daniel Basilio Zandonadi RodrigoLemes Martins Francisco de Assis Esteves |
| author_facet | Mirella Pupo Santos João V.S. Rabelo Araujo Arthur V. Sant’anna Lopes Julio Cesar Fiorio Vettorazzi Marcela Santana Bastos Boechat Fernanda AbreuSantana Arêdes Naiara Viana Campos Emiliano Nicolas Calderon Fernando M. Gomes Santos Tais Nogueira Fernandes Rodrigo Nunes da Fonseca Messias Gonzaga Pereira Guilherme Oliveira Daniel Basilio Zandonadi RodrigoLemes Martins Francisco de Assis Esteves |
| author_sort | Mirella Pupo Santos |
| collection | DOAJ |
| description | Background Two endemic lycophyte species Isoetes cangae and Isoetes serracarajensis have been recently described in the State of Pará in the Amazon forest located in northern Brazil. Isoetes L. has survived through three mass extinctions. Plants are considered small-sized, heterosporous, and can display a great diversity of physiological adaptations to different environments. Thus, the current study aimed to estimate the genetic variation of the populations of I. cangae and I. serracarajensis to generate information about their different mechanisms for survival at the same geographical location that could point to different reproductive, adaptative and dispersal strategies and should be considered for effective conservation strategies. Methods The genetic diversity and population structure of I. cangae and I. serracarajensis were investigated using Inter Simple Sequence Repeat (ISSR) molecular markers. Total genomic DNA was isolated, and the genetic diversity parameters were calculated. Results The sixteen primers produced 115 reproducible bands, 87% of which were polymorphic. A high level of polymorphic loci (81.74% and 68.48%) and a high Shannon index (Sh = 0.376 and 0.289) were observed for I. cangae and I. serracarajensis, respectively. The coefficient of genetic differentiation between population areas (GST) showed a higher value in I. serracarajensis (0.5440). Gene flow was higher in I. cangae (1.715) and lower in I. serracarajensis populations (0.419). Overall, the results further show that I. serracarajensis and I. cangae are two species with considerable genetic variation and that these differences may reflect their habitats and modes of reproduction. These results should be considered in the development of effective conservation strategies for both species. |
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| spelling | doaj.art-41df154e3cff44f1a330d11d75f97d2c2023-12-03T11:00:56ZengPeerJ Inc.PeerJ2167-83592020-11-018e1027410.7717/peerj.10274The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) speciesMirella Pupo Santos0João V.S. Rabelo Araujo1Arthur V. Sant’anna Lopes2Julio Cesar Fiorio Vettorazzi3Marcela Santana Bastos Boechat4Fernanda AbreuSantana Arêdes5Naiara Viana Campos6Emiliano Nicolas Calderon7Fernando M. Gomes Santos8Tais Nogueira Fernandes9Rodrigo Nunes da Fonseca10Messias Gonzaga Pereira11Guilherme Oliveira12Daniel Basilio Zandonadi13RodrigoLemes Martins14Francisco de Assis Esteves15Instituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilLaboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, BrazilLaboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, BrazilLaboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilEnvironmental Studies Office, Vale, Belo Horizonte, Minas Gerais, BrazilEnvironmental Studies Office, Vale, Belo Horizonte, Minas Gerais, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilLaboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, BrazilInstituto Tecnológico Vale, Belém, Pará, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilInstituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, BrazilBackground Two endemic lycophyte species Isoetes cangae and Isoetes serracarajensis have been recently described in the State of Pará in the Amazon forest located in northern Brazil. Isoetes L. has survived through three mass extinctions. Plants are considered small-sized, heterosporous, and can display a great diversity of physiological adaptations to different environments. Thus, the current study aimed to estimate the genetic variation of the populations of I. cangae and I. serracarajensis to generate information about their different mechanisms for survival at the same geographical location that could point to different reproductive, adaptative and dispersal strategies and should be considered for effective conservation strategies. Methods The genetic diversity and population structure of I. cangae and I. serracarajensis were investigated using Inter Simple Sequence Repeat (ISSR) molecular markers. Total genomic DNA was isolated, and the genetic diversity parameters were calculated. Results The sixteen primers produced 115 reproducible bands, 87% of which were polymorphic. A high level of polymorphic loci (81.74% and 68.48%) and a high Shannon index (Sh = 0.376 and 0.289) were observed for I. cangae and I. serracarajensis, respectively. The coefficient of genetic differentiation between population areas (GST) showed a higher value in I. serracarajensis (0.5440). Gene flow was higher in I. cangae (1.715) and lower in I. serracarajensis populations (0.419). Overall, the results further show that I. serracarajensis and I. cangae are two species with considerable genetic variation and that these differences may reflect their habitats and modes of reproduction. These results should be considered in the development of effective conservation strategies for both species.https://peerj.com/articles/10274.pdfIsoetes cangaeIsoetes serracarajensisISSRLycophyteGene flowMating system |
| spellingShingle | Mirella Pupo Santos João V.S. Rabelo Araujo Arthur V. Sant’anna Lopes Julio Cesar Fiorio Vettorazzi Marcela Santana Bastos Boechat Fernanda AbreuSantana Arêdes Naiara Viana Campos Emiliano Nicolas Calderon Fernando M. Gomes Santos Tais Nogueira Fernandes Rodrigo Nunes da Fonseca Messias Gonzaga Pereira Guilherme Oliveira Daniel Basilio Zandonadi RodrigoLemes Martins Francisco de Assis Esteves The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species PeerJ Isoetes cangae Isoetes serracarajensis ISSR Lycophyte Gene flow Mating system |
| title | The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species |
| title_full | The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species |
| title_fullStr | The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species |
| title_full_unstemmed | The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species |
| title_short | The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species |
| title_sort | genetic diversity and population structure of two endemic amazonian quillwort isoetes l species |
| topic | Isoetes cangae Isoetes serracarajensis ISSR Lycophyte Gene flow Mating system |
| url | https://peerj.com/articles/10274.pdf |
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