Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levels
Phosphorus is one of the most important nutrients required for plant growth and development. However, owing to its low availability in the soil, phosphorus is also one of the most difficult elements for plants to acquire. Phosphorus released into the soil from bedrock quickly becomes unavailable to...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-08-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1218617/full |
| _version_ | 1827856352893992960 |
|---|---|
| author | Piotr Koczorski Bliss Ursula Furtado Christel Baum Martin Weih Pär Ingvarsson Piotr Hulisz Katarzyna Hrynkiewicz |
| author_facet | Piotr Koczorski Bliss Ursula Furtado Christel Baum Martin Weih Pär Ingvarsson Piotr Hulisz Katarzyna Hrynkiewicz |
| author_sort | Piotr Koczorski |
| collection | DOAJ |
| description | Phosphorus is one of the most important nutrients required for plant growth and development. However, owing to its low availability in the soil, phosphorus is also one of the most difficult elements for plants to acquire. Phosphorus released into the soil from bedrock quickly becomes unavailable to plants, forming poorly soluble complexes. Phosphate-solubilizing bacteria (PSB) can solubilize unavailable phosphorus-containing compounds into forms in which phosphorus is readily available, thus promoting plant growth. In this study, two willow species, Salix dasyclados cv. Loden and Salix schwerinii × Salix viminalis cv. Tora, were inoculated with two selected bacterial strains, Pantoea agglomerans and Paenibacillus spp., to evaluate the plant growth parameters and changes in gene expression in the presence of different concentrations of tricalcium phosphate: 0 mM (NP), 1 mM (LP), and 2 mM (HP). Inoculation with PSB increased root, shoot and leaf biomass, and for the HP treatment, significant changes in growth patterns were observed. However, the growth responses to plant treatments tested depended on the willow species. Analysis of the leaf transcriptomes of the phosphate-solubilizing bacterium-inoculated plants showed a large variation in gene expression between the two willow species. For the Tora willow species, upregulation of genes was observed, particularly for those involved in pathways related to photosynthesis, and this effect was strongly influenced by bacterial phosphate solubilization. The Loden willow species was characterized by a general downregulation of genes involved in pathway activity that included ion transport, transcription regulation and chromosomes. The results obtained in this study provide an improved understanding of the dynamics of Salix growth and gene expression under the influence of PSB, contributing to an increase in yield and phosphorus-use efficiency. |
| first_indexed | 2024-03-12T12:24:33Z |
| format | Article |
| id | doaj.art-41e414c24d884e5c80bd9f3e06b8954a |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T12:24:33Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-41e414c24d884e5c80bd9f3e06b8954a2023-08-30T01:15:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-08-011410.3389/fpls.2023.12186171218617Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levelsPiotr Koczorski0Bliss Ursula Furtado1Christel Baum2Martin Weih3Pär Ingvarsson4Piotr Hulisz5Katarzyna Hrynkiewicz6Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Torun, PolandDepartment of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Torun, PolandSoil Science, Faculty of Agricultural and Environmental Sciences, University of Rostock, Rostock, GermanyDepartment of Crop Production Ecology, Swedish University of Agricultural Sciences, Uppsala, SwedenLinnean Centre for Plant Biology, Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Science, Uppsala, SwedenDepartment of Soil Science and Landscape Management, Faculty of Earth Sciences and Spatial Management, Nicolaus Copernicus University, Torun, PolandDepartment of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Torun, PolandPhosphorus is one of the most important nutrients required for plant growth and development. However, owing to its low availability in the soil, phosphorus is also one of the most difficult elements for plants to acquire. Phosphorus released into the soil from bedrock quickly becomes unavailable to plants, forming poorly soluble complexes. Phosphate-solubilizing bacteria (PSB) can solubilize unavailable phosphorus-containing compounds into forms in which phosphorus is readily available, thus promoting plant growth. In this study, two willow species, Salix dasyclados cv. Loden and Salix schwerinii × Salix viminalis cv. Tora, were inoculated with two selected bacterial strains, Pantoea agglomerans and Paenibacillus spp., to evaluate the plant growth parameters and changes in gene expression in the presence of different concentrations of tricalcium phosphate: 0 mM (NP), 1 mM (LP), and 2 mM (HP). Inoculation with PSB increased root, shoot and leaf biomass, and for the HP treatment, significant changes in growth patterns were observed. However, the growth responses to plant treatments tested depended on the willow species. Analysis of the leaf transcriptomes of the phosphate-solubilizing bacterium-inoculated plants showed a large variation in gene expression between the two willow species. For the Tora willow species, upregulation of genes was observed, particularly for those involved in pathways related to photosynthesis, and this effect was strongly influenced by bacterial phosphate solubilization. The Loden willow species was characterized by a general downregulation of genes involved in pathway activity that included ion transport, transcription regulation and chromosomes. The results obtained in this study provide an improved understanding of the dynamics of Salix growth and gene expression under the influence of PSB, contributing to an increase in yield and phosphorus-use efficiency.https://www.frontiersin.org/articles/10.3389/fpls.2023.1218617/fulltranscriptome analysiswillowphosphate solubilizationphosphate solubilizing microorganismdifferential gene expression |
| spellingShingle | Piotr Koczorski Bliss Ursula Furtado Christel Baum Martin Weih Pär Ingvarsson Piotr Hulisz Katarzyna Hrynkiewicz Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levels Frontiers in Plant Science transcriptome analysis willow phosphate solubilization phosphate solubilizing microorganism differential gene expression |
| title | Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levels |
| title_full | Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levels |
| title_fullStr | Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levels |
| title_full_unstemmed | Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levels |
| title_short | Large effect of phosphate-solubilizing bacteria on the growth and gene expression of Salix spp. at low phosphorus levels |
| title_sort | large effect of phosphate solubilizing bacteria on the growth and gene expression of salix spp at low phosphorus levels |
| topic | transcriptome analysis willow phosphate solubilization phosphate solubilizing microorganism differential gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1218617/full |
| work_keys_str_mv | AT piotrkoczorski largeeffectofphosphatesolubilizingbacteriaonthegrowthandgeneexpressionofsalixsppatlowphosphoruslevels AT blissursulafurtado largeeffectofphosphatesolubilizingbacteriaonthegrowthandgeneexpressionofsalixsppatlowphosphoruslevels AT christelbaum largeeffectofphosphatesolubilizingbacteriaonthegrowthandgeneexpressionofsalixsppatlowphosphoruslevels AT martinweih largeeffectofphosphatesolubilizingbacteriaonthegrowthandgeneexpressionofsalixsppatlowphosphoruslevels AT paringvarsson largeeffectofphosphatesolubilizingbacteriaonthegrowthandgeneexpressionofsalixsppatlowphosphoruslevels AT piotrhulisz largeeffectofphosphatesolubilizingbacteriaonthegrowthandgeneexpressionofsalixsppatlowphosphoruslevels AT katarzynahrynkiewicz largeeffectofphosphatesolubilizingbacteriaonthegrowthandgeneexpressionofsalixsppatlowphosphoruslevels |