Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein
The mechanism by which proteins are solvated in hydrated ionic liquids remains an open question. Herein, the photoexcitation dynamics of photoactive yellow protein dissolved in hydrated choline dihydrogen phosphate (Hy[ch][dhp]) were studied by transient absorption and transient grating spectroscopy...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/15/4554 |
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| author | Utana Umezaki Miu Hatakenaka Kana Onodera Hiroto Mizutani Suhyang Kim Yusuke Nakasone Masahide Terazima Yoshifumi Kimura |
| author_facet | Utana Umezaki Miu Hatakenaka Kana Onodera Hiroto Mizutani Suhyang Kim Yusuke Nakasone Masahide Terazima Yoshifumi Kimura |
| author_sort | Utana Umezaki |
| collection | DOAJ |
| description | The mechanism by which proteins are solvated in hydrated ionic liquids remains an open question. Herein, the photoexcitation dynamics of photoactive yellow protein dissolved in hydrated choline dihydrogen phosphate (Hy[ch][dhp]) were studied by transient absorption and transient grating spectroscopy. The photocyclic reaction of the protein in Hy[ch][dhp] was similar to that observed in the buffer solution, as confirmed by transient absorption spectroscopy. However, the structural change of the protein during the photocycle in Hy[ch][dhp] was found to be different from that observed in the buffer solution. The known change in the diffusion coefficient of the protein was apparently suppressed in high concentrations of [ch][dhp], plausibly due to stabilization of the secondary structure. |
| first_indexed | 2024-03-10T09:10:58Z |
| format | Article |
| id | doaj.art-41e6754b547043d2849893c473177e90 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T09:10:58Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-41e6754b547043d2849893c473177e902023-11-22T05:59:50ZengMDPI AGMolecules1420-30492021-07-012615455410.3390/molecules26154554Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow ProteinUtana Umezaki0Miu Hatakenaka1Kana Onodera2Hiroto Mizutani3Suhyang Kim4Yusuke Nakasone5Masahide Terazima6Yoshifumi Kimura7Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe 610-0321, JapanDepartment of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe 610-0321, JapanDepartment of Applied Chemistry, Graduate School of Science and Engineering, Doshisha University, Kyotanabe 610-0321, JapanDepartment of Applied Chemistry, Graduate School of Science and Engineering, Doshisha University, Kyotanabe 610-0321, JapanDepartment of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, JapanDepartment of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, JapanDepartment of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, JapanDepartment of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University, Kyotanabe 610-0321, JapanThe mechanism by which proteins are solvated in hydrated ionic liquids remains an open question. Herein, the photoexcitation dynamics of photoactive yellow protein dissolved in hydrated choline dihydrogen phosphate (Hy[ch][dhp]) were studied by transient absorption and transient grating spectroscopy. The photocyclic reaction of the protein in Hy[ch][dhp] was similar to that observed in the buffer solution, as confirmed by transient absorption spectroscopy. However, the structural change of the protein during the photocycle in Hy[ch][dhp] was found to be different from that observed in the buffer solution. The known change in the diffusion coefficient of the protein was apparently suppressed in high concentrations of [ch][dhp], plausibly due to stabilization of the secondary structure.https://www.mdpi.com/1420-3049/26/15/4554hydrated ionic liquidphotoactive yellow proteinphotocycletransient grating spectroscopyconformational change |
| spellingShingle | Utana Umezaki Miu Hatakenaka Kana Onodera Hiroto Mizutani Suhyang Kim Yusuke Nakasone Masahide Terazima Yoshifumi Kimura Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein Molecules hydrated ionic liquid photoactive yellow protein photocycle transient grating spectroscopy conformational change |
| title | Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein |
| title_full | Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein |
| title_fullStr | Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein |
| title_full_unstemmed | Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein |
| title_short | Effect of Hydrated Ionic Liquid on Photocycle and Dynamics of Photoactive Yellow Protein |
| title_sort | effect of hydrated ionic liquid on photocycle and dynamics of photoactive yellow protein |
| topic | hydrated ionic liquid photoactive yellow protein photocycle transient grating spectroscopy conformational change |
| url | https://www.mdpi.com/1420-3049/26/15/4554 |
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