SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model
We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based on knowledge of target sequence provided expected results w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2003-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200304-0002_snp-typing-in-cereals-comparison-of-sscp-and-snapshot-markers-using-the-barley-mlo-locus-as-a-model.php |
| _version_ | 1797899531016208384 |
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| author | J. Ovesná K. Poláková L. Kučera J. Rulcová |
| author_facet | J. Ovesná K. Poláková L. Kučera J. Rulcová |
| author_sort | J. Ovesná |
| collection | DOAJ |
| description | We compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based on knowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Analyser. |
| first_indexed | 2024-04-10T08:31:24Z |
| format | Article |
| id | doaj.art-41e7daa54f5e43d287a4e1a62f10c725 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:31:24Z |
| publishDate | 2003-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-41e7daa54f5e43d287a4e1a62f10c7252023-02-23T03:28:57ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252003-12-0139410911210.17221/3727-CJGPBcjg-200304-0002SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a ModelJ. Ovesná0K. Poláková1L. Kučera2J. Rulcová3Division of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicDivision of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicDivision of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicDivision of Genetics and Plant Breeding, Research Institute of Crop Production, Prague-Ruzyně, Czech RepublicWe compared the applicability of SSCP and SNaPShot markers in barley Mlo loci as a model of SNP detection in cereals. Whereas the development of SSCP markers required optimisation steps, the ddNTP primer extension (SNaPShot) procedure based on knowledge of target sequence provided expected results without any previous optimisations. We have shown, that SNPs can be easily scored using the ABI PRISM 310 Genetic Analyser.https://cjgpb.agriculturejournals.cz/artkey/cjg-200304-0002_snp-typing-in-cereals-comparison-of-sscp-and-snapshot-markers-using-the-barley-mlo-locus-as-a-model.phpsscpsnapshotmlosnpdna markerbarleyprimer extension |
| spellingShingle | J. Ovesná K. Poláková L. Kučera J. Rulcová SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model Czech Journal of Genetics and Plant Breeding sscp snapshot mlo snp dna marker barley primer extension |
| title | SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model |
| title_full | SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model |
| title_fullStr | SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model |
| title_full_unstemmed | SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model |
| title_short | SNP Typing in Cereals: Comparison of SSCP and SNaPShot Markers Using the Barley Mlo Locus as a Model |
| title_sort | snp typing in cereals comparison of sscp and snapshot markers using the barley mlo locus as a model |
| topic | sscp snapshot mlo snp dna marker barley primer extension |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200304-0002_snp-typing-in-cereals-comparison-of-sscp-and-snapshot-markers-using-the-barley-mlo-locus-as-a-model.php |
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