The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans.
UBR1 is an E3 ubiquitin ligase best known for its ability to target protein degradation by the N-end rule. The physiological functions of UBR family proteins, however, remain not fully understood. We found that the functional loss of C. elegans UBR-1 leads to a specific motor deficit: when adult ani...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-04-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5931689?pdf=render |
| _version_ | 1818838570619633664 |
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| author | Jyothsna Chitturi Wesley Hung Anas M Abdel Rahman Min Wu Maria A Lim John Calarco Renee Baran Xun Huang James W Dennis Mei Zhen |
| author_facet | Jyothsna Chitturi Wesley Hung Anas M Abdel Rahman Min Wu Maria A Lim John Calarco Renee Baran Xun Huang James W Dennis Mei Zhen |
| author_sort | Jyothsna Chitturi |
| collection | DOAJ |
| description | UBR1 is an E3 ubiquitin ligase best known for its ability to target protein degradation by the N-end rule. The physiological functions of UBR family proteins, however, remain not fully understood. We found that the functional loss of C. elegans UBR-1 leads to a specific motor deficit: when adult animals generate reversal movements, A-class motor neurons exhibit synchronized activation, preventing body bending. This motor deficit is rescued by removing GOT-1, a transaminase that converts aspartate to glutamate. Both UBR-1 and GOT-1 are expressed and critically required in premotor interneurons of the reversal motor circuit to regulate the motor pattern. ubr-1 and got-1 mutants exhibit elevated and decreased glutamate level, respectively. These results raise an intriguing possibility that UBR proteins regulate glutamate metabolism, which is critical for neuronal development and signaling. |
| first_indexed | 2024-12-19T03:40:30Z |
| format | Article |
| id | doaj.art-41e7ddb2774043c3a19a4f785964a3e6 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-19T03:40:30Z |
| publishDate | 2018-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-41e7ddb2774043c3a19a4f785964a3e62022-12-21T20:37:15ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042018-04-01144e100730310.1371/journal.pgen.1007303The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans.Jyothsna ChitturiWesley HungAnas M Abdel RahmanMin WuMaria A LimJohn CalarcoRenee BaranXun HuangJames W DennisMei ZhenUBR1 is an E3 ubiquitin ligase best known for its ability to target protein degradation by the N-end rule. The physiological functions of UBR family proteins, however, remain not fully understood. We found that the functional loss of C. elegans UBR-1 leads to a specific motor deficit: when adult animals generate reversal movements, A-class motor neurons exhibit synchronized activation, preventing body bending. This motor deficit is rescued by removing GOT-1, a transaminase that converts aspartate to glutamate. Both UBR-1 and GOT-1 are expressed and critically required in premotor interneurons of the reversal motor circuit to regulate the motor pattern. ubr-1 and got-1 mutants exhibit elevated and decreased glutamate level, respectively. These results raise an intriguing possibility that UBR proteins regulate glutamate metabolism, which is critical for neuronal development and signaling.http://europepmc.org/articles/PMC5931689?pdf=render |
| spellingShingle | Jyothsna Chitturi Wesley Hung Anas M Abdel Rahman Min Wu Maria A Lim John Calarco Renee Baran Xun Huang James W Dennis Mei Zhen The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans. PLoS Genetics |
| title | The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans. |
| title_full | The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans. |
| title_fullStr | The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans. |
| title_full_unstemmed | The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans. |
| title_short | The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in Caenorhabditis elegans. |
| title_sort | ubr 1 ubiquitin ligase regulates glutamate metabolism to generate coordinated motor pattern in caenorhabditis elegans |
| url | http://europepmc.org/articles/PMC5931689?pdf=render |
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