Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis
<p>Abstract</p> <p>Background</p> <p>Prostanoids are known to participate in the process of fibrogenesis. Because lung fibroblasts produce prostanoids and are believed to play a central role in the pathogenesis of idiopathic pulmonary fibrosis (IPF), we hypothesized tha...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2002-02-01
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| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1186/rr166 |
| _version_ | 1818473271784374272 |
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| author | Pierson Richard Loyd James E Lane Kirk B Dworski Ryszard Stecenko Arlene A Cruz-Gervis Roberto King Gayle Brigham Kenneth L |
| author_facet | Pierson Richard Loyd James E Lane Kirk B Dworski Ryszard Stecenko Arlene A Cruz-Gervis Roberto King Gayle Brigham Kenneth L |
| author_sort | Pierson Richard |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Prostanoids are known to participate in the process of fibrogenesis. Because lung fibroblasts produce prostanoids and are believed to play a central role in the pathogenesis of idiopathic pulmonary fibrosis (IPF), we hypothesized that fibroblasts (HF) cultured from the lungs of patients with IPF (HF-IPF) have an altered balance between profibrotic (thromboxane [TX]A<sub>2</sub>) and antifibrotic (prostacyclin [PGI<sub>2</sub>]) prostaglandins (PGs) when compared with normal human lung fibroblasts (HF-NL).</p> <p>Methods</p> <p>We measured inducible cyclooxygenase (COX)-2 gene and protein expression, and a profile of prostanoids at baseline and after IL-1β stimulation.</p> <p>Results</p> <p>In both HF-IPF and HF-NL COX-2 expression was undetectable at baseline, but was significantly upregulated by IL-1β. PGE<sub>2</sub> was the predominant COX product in IL-1β-stimulated cells with no significant difference between HF-IPF and HF-NL (28.35 [9.09–89.09] vs. 17.12 [8.58–29.33] ng/10<sup>6</sup> cells/30 min, respectively; <it>P</it> = 0.25). TXB<sub>2</sub> (the stable metabolite of TXA<sub>2</sub>) production was significantly higher in IL-1β-stimulated HF-IPF compared to HF-NL (1.92 [1.27–2.57] vs. 0.61 [0.21–1.64] ng/10<sup>6</sup> cells/30 min, respectively; <it>P</it> = 0.007) and the ratio of PGI<sub>2</sub> (as measured by its stable metabolite 6-keto-PGF<sub>1α</sub>) to TXB<sub>2</sub> was significantly lower at baseline in HF-IPF (0.08 [0.04–0.52] vs. 0.12 [0.11–0.89] in HF-NL; <it>P</it> = 0.028) and with IL-1β stimulation (0.24 [0.05–1.53] vs. 1.08 [0.51–3.79] in HF-NL; <it>P</it> = 0.09).</p> <p>Conclusion</p> <p>An alteration in the balance of profibrotic and antifibrotic PGs in HF-IPF may play a role in the pathogeneses of IPF.</p> |
| first_indexed | 2024-04-14T04:21:46Z |
| format | Article |
| id | doaj.art-41eeefec71294be49c3f2c426fc4f4db |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-14T04:21:46Z |
| publishDate | 2002-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-41eeefec71294be49c3f2c426fc4f4db2022-12-22T02:12:30ZengBMCRespiratory Research1465-99212002-02-01311710.1186/rr166Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosisPierson RichardLoyd James ELane Kirk BDworski RyszardStecenko Arlene ACruz-Gervis RobertoKing GayleBrigham Kenneth L<p>Abstract</p> <p>Background</p> <p>Prostanoids are known to participate in the process of fibrogenesis. Because lung fibroblasts produce prostanoids and are believed to play a central role in the pathogenesis of idiopathic pulmonary fibrosis (IPF), we hypothesized that fibroblasts (HF) cultured from the lungs of patients with IPF (HF-IPF) have an altered balance between profibrotic (thromboxane [TX]A<sub>2</sub>) and antifibrotic (prostacyclin [PGI<sub>2</sub>]) prostaglandins (PGs) when compared with normal human lung fibroblasts (HF-NL).</p> <p>Methods</p> <p>We measured inducible cyclooxygenase (COX)-2 gene and protein expression, and a profile of prostanoids at baseline and after IL-1β stimulation.</p> <p>Results</p> <p>In both HF-IPF and HF-NL COX-2 expression was undetectable at baseline, but was significantly upregulated by IL-1β. PGE<sub>2</sub> was the predominant COX product in IL-1β-stimulated cells with no significant difference between HF-IPF and HF-NL (28.35 [9.09–89.09] vs. 17.12 [8.58–29.33] ng/10<sup>6</sup> cells/30 min, respectively; <it>P</it> = 0.25). TXB<sub>2</sub> (the stable metabolite of TXA<sub>2</sub>) production was significantly higher in IL-1β-stimulated HF-IPF compared to HF-NL (1.92 [1.27–2.57] vs. 0.61 [0.21–1.64] ng/10<sup>6</sup> cells/30 min, respectively; <it>P</it> = 0.007) and the ratio of PGI<sub>2</sub> (as measured by its stable metabolite 6-keto-PGF<sub>1α</sub>) to TXB<sub>2</sub> was significantly lower at baseline in HF-IPF (0.08 [0.04–0.52] vs. 0.12 [0.11–0.89] in HF-NL; <it>P</it> = 0.028) and with IL-1β stimulation (0.24 [0.05–1.53] vs. 1.08 [0.51–3.79] in HF-NL; <it>P</it> = 0.09).</p> <p>Conclusion</p> <p>An alteration in the balance of profibrotic and antifibrotic PGs in HF-IPF may play a role in the pathogeneses of IPF.</p>http://dx.doi.org/10.1186/rr166lung fibroblastsprostacyclinprostaglandinspulmonary fibrosisthromboxane |
| spellingShingle | Pierson Richard Loyd James E Lane Kirk B Dworski Ryszard Stecenko Arlene A Cruz-Gervis Roberto King Gayle Brigham Kenneth L Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis Respiratory Research lung fibroblasts prostacyclin prostaglandins pulmonary fibrosis thromboxane |
| title | Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis |
| title_full | Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis |
| title_fullStr | Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis |
| title_full_unstemmed | Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis |
| title_short | Altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis |
| title_sort | altered prostanoid production by fibroblasts cultured from the lungs of human subjects with idiopathic pulmonary fibrosis |
| topic | lung fibroblasts prostacyclin prostaglandins pulmonary fibrosis thromboxane |
| url | http://dx.doi.org/10.1186/rr166 |
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