Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity
We have previously discovered a long-lasting enhancement of synaptic transmission in mammal autonomic ganglia caused by immunological activation of ganglionic mast cells. Subsequent to mast cell activation, lipid and peptide mediators are released which may modulate synaptic function. In this study...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1997-07-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700014 |
| _version_ | 1828438285250199552 |
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| author | A.A.C. Albuquerque J.H. Leal-Cardoso D. Weinreich |
| author_facet | A.A.C. Albuquerque J.H. Leal-Cardoso D. Weinreich |
| author_sort | A.A.C. Albuquerque |
| collection | DOAJ |
| description | We have previously discovered a long-lasting enhancement of synaptic transmission in mammal autonomic ganglia caused by immunological activation of ganglionic mast cells. Subsequent to mast cell activation, lipid and peptide mediators are released which may modulate synaptic function. In this study we determined whether some mast cell-derived mediators, prostaglandin D2 (PGD2; 1.0 µM), platelet aggregating factor (PAF; 0.3 µM) and U44619 (a thromboxane analogue; 1.0 µM), and also endothelin-1 (ET-1; 0.5 µM) induce synaptic potentiation in the guinea pig superior cervical ganglion (SCG), and compared their effects on synaptic transmission with those induced by a sensitizing antigen, ovalbumin (OVA; 10 µg/ml). The experiments were carried out on SCGs isolated from adult male guinea pigs (200-250 g) actively sensitized to OVA, maintained in oxygenated Locke solution at 37oC. Synaptic potentiation was measured through alterations of the integral of the post-ganglionic compound action potential (CAP). All agents tested caused long-term (LTP; duration <FONT FACE="Symbol">³</font>30 min) or short-term (STP; <30 min) potentiation of synaptic efficacy, as measured by the increase in the integral of the post-ganglionic CAP. The magnitude of mediator-induced potentiation was never the same as the antigen-induced long-term potentiation (A-LTP). The agent that best mimicked the antigen was PGD2, which induced a 75% increase in CAP integral for LTP (antigen: 94%) and a 34% increase for STP (antigen: 91%). PAF-, U44619-, and ET-1-induced increases in CAP integral ranged for LTP from 34 to 47%, and for STP from 0 to 26%. These results suggest that the agents investigated may participate in the induction of A-LTP |
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| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-10T20:02:05Z |
| publishDate | 1997-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-41ef87a2570c4e19b09edaf7caab84df2022-12-22T01:35:30ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X1997-07-0130790910.1590/S0100-879X1997000700014Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticityA.A.C. AlbuquerqueJ.H. Leal-CardosoD. WeinreichWe have previously discovered a long-lasting enhancement of synaptic transmission in mammal autonomic ganglia caused by immunological activation of ganglionic mast cells. Subsequent to mast cell activation, lipid and peptide mediators are released which may modulate synaptic function. In this study we determined whether some mast cell-derived mediators, prostaglandin D2 (PGD2; 1.0 µM), platelet aggregating factor (PAF; 0.3 µM) and U44619 (a thromboxane analogue; 1.0 µM), and also endothelin-1 (ET-1; 0.5 µM) induce synaptic potentiation in the guinea pig superior cervical ganglion (SCG), and compared their effects on synaptic transmission with those induced by a sensitizing antigen, ovalbumin (OVA; 10 µg/ml). The experiments were carried out on SCGs isolated from adult male guinea pigs (200-250 g) actively sensitized to OVA, maintained in oxygenated Locke solution at 37oC. Synaptic potentiation was measured through alterations of the integral of the post-ganglionic compound action potential (CAP). All agents tested caused long-term (LTP; duration <FONT FACE="Symbol">³</font>30 min) or short-term (STP; <30 min) potentiation of synaptic efficacy, as measured by the increase in the integral of the post-ganglionic CAP. The magnitude of mediator-induced potentiation was never the same as the antigen-induced long-term potentiation (A-LTP). The agent that best mimicked the antigen was PGD2, which induced a 75% increase in CAP integral for LTP (antigen: 94%) and a 34% increase for STP (antigen: 91%). PAF-, U44619-, and ET-1-induced increases in CAP integral ranged for LTP from 34 to 47%, and for STP from 0 to 26%. These results suggest that the agents investigated may participate in the induction of A-LTPhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700014prostaglandin Dplatelet aggregating factorendothelin-1thromboxanemast cellsynaptic plasticity |
| spellingShingle | A.A.C. Albuquerque J.H. Leal-Cardoso D. Weinreich Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity Brazilian Journal of Medical and Biological Research prostaglandin D platelet aggregating factor endothelin-1 thromboxane mast cell synaptic plasticity |
| title | Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity |
| title_full | Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity |
| title_fullStr | Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity |
| title_full_unstemmed | Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity |
| title_short | Role of mast cell- and non-mast cell-derived inflammatory mediators in immunologic induction of synaptic plasticity |
| title_sort | role of mast cell and non mast cell derived inflammatory mediators in immunologic induction of synaptic plasticity |
| topic | prostaglandin D platelet aggregating factor endothelin-1 thromboxane mast cell synaptic plasticity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700014 |
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