| Summary: | <i>Rosa rugosa</i> was a famous aromatic plant while poor salt tolerance of commercial cultivars has hindered its culture in saline-alkali soil. In many plants, the roles of <i>GT</i> (or <i>trihelix</i>) genes in salt stresses responses have been emerging. In the wild <i>R. rugosa,</i> a total of 37 <i>GTs</i> (<i>RrGTs</i>) were grouped into GT-1, GT-2, GTγ, SH4, and SIP1 lineages. SIP1 lineage expanded by transposition. The motifs involved in the binding of GT cis-elements were conserved. Four <i>RrGTs</i> (<i>RrGT11/14/16/18</i>) significantly differentially expressed in roots or leaves under salt stress. The responsive patterns within 8 h NaCl treatment indicated that <i>RrGTγ-4</i> (<i>RrGT18</i>) and <i>RrGT-1</i> (<i>RrGT16</i>) were significantly induced by salt in roots of <i>R. rugosa</i>. Subcellular localizations of <i>RrSIP1</i> (<i>RrGT11</i>) and <i>RrGTγ-4</i> were on chloroplasts while <i>RrGT-1</i> and <i>RrSIP2</i> (<i>RrGT14</i>) located on cell nucleus. Regulation of ion transport could be the most important role of <i>RrSIPs</i> and <i>RrGTγ-4</i>. And <i>RrGT-1</i> could be a halophytic gene with higher transcription abundance than glycophytic <i>GT-1</i>. These results provide key clue for further investigations of roles of <i>RrGTs</i> in salt stress response and would be helpful in the understanding the salt tolerance regulation mechanism of <i>R. rugosa</i>.
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