Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBs
The environment contains a multitude of man-made chemicals, some of which can act as endocrine disruptors (EDCs), while others can be immunotoxic. We evaluated thyroid disruption and immunotoxic effects in wild female perch (Perca fluviatilis) collected from two contaminated areas in Sweden; one sit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321006072 |
| _version_ | 1818911770431979520 |
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| author | Lina Birgersson Justin Jouve Elisabeth Jönsson Noomi Asker Fredrik Andreasson Oksana Golovko Lutz Ahrens Joachim Sturve |
| author_facet | Lina Birgersson Justin Jouve Elisabeth Jönsson Noomi Asker Fredrik Andreasson Oksana Golovko Lutz Ahrens Joachim Sturve |
| author_sort | Lina Birgersson |
| collection | DOAJ |
| description | The environment contains a multitude of man-made chemicals, some of which can act as endocrine disruptors (EDCs), while others can be immunotoxic. We evaluated thyroid disruption and immunotoxic effects in wild female perch (Perca fluviatilis) collected from two contaminated areas in Sweden; one site contaminated with per- and polyfluoroalkyl substances (PFASs) and two sites contaminated with polychlorinated biphenyls (PCBs), with one reference site included for each area. The hepatic mRNA expression of thyroid receptors α and β, and the thyroid hormone metabolising iodothyronine deiodinases (dio1, dio2 and dio3) were measured using real-time PCR, while the levels of thyroid hormone T3 in plasma was analysed using a radioimmunoassay. In addition, lymphocytes, granulocytes, and thrombocytes were counted microscopically. Our results showed lower levels of T3 as well as lower amounts of lymphocytes and granulocytes in perch collected from the PFAS-contaminated site compared to reference sites. In addition, expressions of mRNA coding for thyroid hormone metabolising enzymes (dio2 and dio3) and thyroid receptor α (thra) were significantly different in these fish compared to their reference site. For perch collected at the two PCB-contaminated sites, there were no significant differences in T3 levels or in expression levels of the thyroid-related genes, compared to the reference fish. Fish from one of the PCB-contaminated sites had higher levels of thrombocytes compared with both the second PCB lake and their reference lake; hence PCBs are unlikely to be the cause of this effect. The current study suggests that lifelong exposure to PFASs could affect both the thyroid hormone status and immune defence of perch in the wild. |
| first_indexed | 2024-12-19T23:03:59Z |
| format | Article |
| id | doaj.art-41f769dad5f1411785647caff8958364 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-19T23:03:59Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-41f769dad5f1411785647caff89583642022-12-21T20:02:26ZengElsevierEcotoxicology and Environmental Safety0147-65132021-10-01222112495Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBsLina Birgersson0Justin Jouve1Elisabeth Jönsson2Noomi Asker3Fredrik Andreasson4Oksana Golovko5Lutz Ahrens6Joachim Sturve7Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Göteborg, SwedenDepartment of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Göteborg, SwedenDepartment of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Göteborg, SwedenDepartment of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Göteborg, SwedenDepartment for Nature and Climate, County Administrative Board of Blekinge, SE-371 86 Karlskrona, SwedenDepartment of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences (SLU), SE-75 007 Uppsala, SwedenDepartment of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences (SLU), SE-75 007 Uppsala, SwedenDepartment of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Göteborg, Sweden; Corresponding author.The environment contains a multitude of man-made chemicals, some of which can act as endocrine disruptors (EDCs), while others can be immunotoxic. We evaluated thyroid disruption and immunotoxic effects in wild female perch (Perca fluviatilis) collected from two contaminated areas in Sweden; one site contaminated with per- and polyfluoroalkyl substances (PFASs) and two sites contaminated with polychlorinated biphenyls (PCBs), with one reference site included for each area. The hepatic mRNA expression of thyroid receptors α and β, and the thyroid hormone metabolising iodothyronine deiodinases (dio1, dio2 and dio3) were measured using real-time PCR, while the levels of thyroid hormone T3 in plasma was analysed using a radioimmunoassay. In addition, lymphocytes, granulocytes, and thrombocytes were counted microscopically. Our results showed lower levels of T3 as well as lower amounts of lymphocytes and granulocytes in perch collected from the PFAS-contaminated site compared to reference sites. In addition, expressions of mRNA coding for thyroid hormone metabolising enzymes (dio2 and dio3) and thyroid receptor α (thra) were significantly different in these fish compared to their reference site. For perch collected at the two PCB-contaminated sites, there were no significant differences in T3 levels or in expression levels of the thyroid-related genes, compared to the reference fish. Fish from one of the PCB-contaminated sites had higher levels of thrombocytes compared with both the second PCB lake and their reference lake; hence PCBs are unlikely to be the cause of this effect. The current study suggests that lifelong exposure to PFASs could affect both the thyroid hormone status and immune defence of perch in the wild.http://www.sciencedirect.com/science/article/pii/S0147651321006072European perchThyroid disruptionImmunotoxicityPFASsPCBs |
| spellingShingle | Lina Birgersson Justin Jouve Elisabeth Jönsson Noomi Asker Fredrik Andreasson Oksana Golovko Lutz Ahrens Joachim Sturve Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBs Ecotoxicology and Environmental Safety European perch Thyroid disruption Immunotoxicity PFASs PCBs |
| title | Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBs |
| title_full | Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBs |
| title_fullStr | Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBs |
| title_full_unstemmed | Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBs |
| title_short | Thyroid function and immune status in perch (Perca fluviatilis) from lakes contaminated with PFASs or PCBs |
| title_sort | thyroid function and immune status in perch perca fluviatilis from lakes contaminated with pfass or pcbs |
| topic | European perch Thyroid disruption Immunotoxicity PFASs PCBs |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321006072 |
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